Journal of Medical Bacteriology 2017. 6(3, 4):23-30.

Antagonistic Activity of Probiotic Bacteria Isolated from Traditional Dairy Products against E. coli O157:H7
Maryam Rahimpour Hesari, Reza Kazemi Darsanaki, Ali Salehzadeh


Background:    Probiotics are living microorganisms that have useful effects on health of digestive system when acquired in a defined dosage. E. coli O157:H7 is known as one of the most important agents of diarrhea in developing countries. Therefore, attention to the treatment of such gastrointestinal disease is essential. The aim of this study was to determine antagonistic activity of food born lactobacilli against E. coli O157:H7.

Methods:     Lactobacilli were isolated from traditional dairy products (yogurt and buttermilk samples). Then, they were characterized using biochemical and molecular tests. Bifidobacterium bifidum PTCC 1644 was obtained from the microbial collection of Iranian Research Organization for Science and Technology in Lyophilized form. Similarly, E. coli O157:H7 PTCC12900 was obtained from faculty of veterinary medicine university of Tehran. The antagonistic activity of probiotics supernatants against E. coli O157:H7 was investigated using the disk diffusion agar, well diffusion agar and pour plate methods.

Results:   The isolates were characterized as Lactobacillus plantarum and Lactobacillus fermentum. All isolates showed antagonistic activities against E. coli O157:H7 in all of the three methods, where the activity of L. plantarum and B. bidifum PTCC 1644 was greater than that of L. fermentum.

Conclusion:   Metabolites produced by the probiotic bacteria are able to inhibit the growth of E. coli O157:H7. This can be an important solution for the prevention and treatment of E. coli O157:H7 infection and ultimately improve human health.


Antagonistic activity, E. coli O157:H7, Probiotic bacteria, PCR, Traditional dairy products.

Full Text:



Rufter J. lactic acid bacteria and cancer. J Nutrition 2002; 88: 589-594.

Çaglar E, Kargul B, Tanboga I. Bacteriotherapy and probiotics’ role on oral health. Oral Diseases 2005; 11(3): 131-137.

Kazemi Darsanaki R, Issazadeh K, et al. Antimutagenic activity of Lactobacillus spp. isolated from fresh vegetables against sodium azide and 2-Nitrofluorene. J Pure Appl Microbio 2012; 6: 1677-1682.

Dos Reis SA, da Conceição LL, Siqueira NP, et al. Review of the mechanisms of probiotic actions in the prevention of colorectal cancer. Nutr Res 2017; 37: 1-19.

Day AS, Keenan JI. Probiotic-mediated modulation of host inflammation. Expert Rev. Gastroenterol. Hepatol 2011; 5(3): 319-321.

Ng SC, Hart AL, Kamm MA, et al. Mechanism action of probiotics: recent advances. Inflamm Bowel Dis 2008; 15(2): 300-305.

Dimidi E, Christodoulides S, Scott SM, et al. Mechanisms of action of probiotics and the gastrointestinal microbiota on gut motility and constipation. Adv Nutr 2017; 8(3): 484-94.

De Roos NM, Katan MB. Effects of probiotic bacteria on human. J Clin Nutr 2000; 171: 405-411.

Boris S, Jimenez-Diaz R, Caso JL, et al. Partial characterization of a bacteriocin produced by Lactobacillus delbrueckii subsp. lactis U0004, An intestinal isolate with probiotic potential. J Appl Microbiol 2001; 91(2): 32-33.

Coconnier MH, Lievin V, Hemery E, et al. Antagonistic activity against Helicobacter infection in vitro and in vivo by human Lactobacillus acidophilus strain LB. Appl Environ Microbiol 1998; 64: 4573-4580.

Ogunbanwo ST, Sanni AI, Onilude AA. Characterization of bacteriocin produced by Lactobacillus plantarum F1 and Lactobacillus brevis OG1. Afr J Biotechnol 2003; 2(8): 219-227.

Ouwehand AC, Kirjavainen PV, Shortt C, et al. Probiotic: mechanisms and established effects. Int Dairy J 1999; 9: 43-52.

Savadogo A, Cheik A. Antimicrobial activities of lactic acid bacteria strains isolated from Burkina Faso fermented milk. Pak. J. Nutr 2004; 3(3): 174-179.

Kazemi Darsanaki R, Rokhi ML, Azizollahi-Aliabadi M, et al. Antimicrobial activities of Lactobacillus strains isolated from fresh vegetables. Middle East J Sci Res 2012; 11(9): 1216-1219.

Strus M, Pakosz K, Gosciniak H. Antagonistic activity of Lactobacillus bacteria strains against anaerobic gastrointestinal tract pathogens (Helicobacter pylori, Campilobacter coli, Campilobacter jejuni and Clostridium difficile). Med Dosw Mikrobiol 2001; 53(2): 133-142.

Sahshorpour M, Amani J, Jafari M, et al. Expression of recombinant chimeric EspA-intimin protein in Nicotiana tobaccum for oral vaccine development. Journal of Genetic Plant Research 2014; 1(1): 77-94.

Ota A. Protection against an infectious disease by Enterohemorrhagic Escherichia coli. Med Hypotheses 1999; 53(1): 87-88.

Zhao T, Doyle MP, Harmon BG, et al. Reduction of carriage of enterohemorrhagic Escherichia coli O157: H7 in cattle by inoculation with probiotic bacteria. J Clin Microbiol 1998; 36(3): 641-647.

Gagnon M, Kheadr EE, Le Blay G, et al. In vitro inhibition of Escherichia coli O157: H7 by bifidobacterial strains of human origin. Int J Food Microbiol 2004; 92(1): 69-78.

Brashears M, Jaroni D, Trimble J. Isolation, selection, and characterization of lactic acid bacteria for a competitive exclusion product to reduce shedding of Escherichia coli O157: H7 in cattle. J Food Prot 2003; 66(3): 355-363.

Matsusaki H, Endo N, Sonomoto K, et al. Lantibiotic nisin Z fermentative production by Lactococcus lactis IO-1: relationship between production of the lantibiotic and lactate and cell growth. Appl Microbiol Biotechnol 1996; 45(1-2): 36-40.

Chakoosari MM, Faezi Ghasemi M, Masiha A. Antimicrobial effect of lactic acid bacteria against common pathogenic bacteria. Bull. Env. Pharmacol. Life Sci 2015; 9(5): 1-4.

Sánchez B, Delgado S, Blanco-Míguez A, et al. Probiotics, gut microbiota, and their influence on host health and disease. Mol Nutr Food Res 2017; 61(1): 1-15.

Iglesias MB, Abadias M, Anguera M, et al. Antagonistic effect of probiotic bacteria against foodborne pathogens on fresh-cut pear. LWT- Food sci. technol 2017; 81: 243-249.

Jørgensen MR, Kragelund C, Jensen PØ, et al. Probiotic Lactobacillus reuteri has antifungal effects on oral Candida species in vitro. J Oral Microbiol 2017; 9(1): 1-8.

Kang MS, Lim HS, et al. Antimicrobial activity of Lactobacillus salivarius and Lactobacillus fermentum against Staphylococcus aureus. Pathog Dis 2017; 75(2): 1-10.

Shu Q, Gill HS. A dietary probiotic (Bifidobacterium lactis HN019) reduces the severity of Escherichia coli O157: H7 infection in mice. Med Microbiol Immun 2001; 189(3): 147-152.


  • There are currently no refbacks.

Creative Commons Attribution-NonCommercial 3.0

This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly.