Journal of Medical Bacteriology 2018. 7(1-2):13-21.

Antibacterial Activity of Ethyl Acetate and Methanol Extracts of Securigera securidaca, Withania sominefra, Rosmarinus officinalis and Aloe vera Plants against Important Human Pathogens
Khadije Rezaie Keikhaie, Bahman Fazeli-Nasab, Hamid Reza Jahantigh, Mehdi Hassanshahian

Abstract


Background:      Considering the importance of new antibiotics, the potential antibacterial activity of ethyl acetate and Methanol extracts of Securigera securidaca, Withania sominefra, Rosmarinus officinalis and Aloe vera plants against important human pathogens was studied.  

Methods:   Streptococcus pneumoniae, Enterococcus faecalis, Proteus mirabilis, Staphylococcus aureus, E. coli, P. aeruginosa were the pathogenic bacteria used to determine the antibacterial effect of ethyl acetate and mathanol extracts of Securigera securidaca, Withania sominefra, Rosmarinus officinalis and Aloe vera in broth micro-dilution method.

Results:      The results of studying the methanol extract of S. securidaca with the lowest inhibitory concentration of 25, showed that all used bacterial pathogens were inhibited at this concentration, while the lowest inhibitory concentration of ethyl acetate extract of Securigera securidaca was 12.5, which were P. mirabilis and S. aureus is inhibited in this concentration. The highest bactericidal concentration (MBC) of S. securidaca ethyl acetate extract was 100, and S. aureus, P. aeruginosa were inhibitory at this concentration. The lowest inhibitory concentration of methanol extract of W. sominefra was 6.25, which S. pneumoniae, P. mirabilis, S. aureus and E. coli bacteria were inhibited at this concentration, while the highest inhibitory concentration was observed against P. aeruginosa (25 ppm), The lowest inhibitory concentration of the ethyl acetate W. sominefra extract was 3.1ppm in comparison with P. mirabilis and E. coli.

Conclusion:     In conclusion, it seems that S. securidaca, W. sominefra, R. officinalis and Aloe vera extracts could inhibit the growth of all of the mentioned bacteria.


Keywords


Antibacterial activity, Human pathogen, Inhibition, Plant extract.

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References


Renu S, Manvi M, Sapna B. Evaluation of antibacterial potential of stem and bark of Moringa oleifera lam. The Bioscan 2010; 1: 89-94.

Mahesh B, Satish S. Antimicrobial activity of some important medicinal plant against plant and human pathogens. World J Agric Sci 2008; 4:839-43.

Mehrotra V, Mehrotra S, Kirar V, et al. Antioxidant and antimicrobial activities of aqueous extract of Withania somnifera against methicillin resistance Staphylcoccus aureus. J Microbiol Biotechnol Res 2011; 1:40-5.

Rezaie Keikhaie K, Bagheri G, Hassanshahian M, et al. Antimicrobial effects of Zataria multiflora essential oils on Acinetobacter strains isolated from clinical specimens. J Herb Drug 2018; 8(4):251-6.

Mohsenipour Z, Hassanshahian M. Antibacterial activity of Euphorbia hebecarpa alcoholic extracts against six human pathogenic bacteria in planktonic and biofilm forms. Jundi J Microbiol 2016; 9(6):e34701.

Sadeghian I, Hassanshahian M, Sadeghian S, et al. Antimicrobial effects of Quercus brantii fruits on bacterial pathogens. Jundi J Microbiol 2012; 5(3):465-9.

Hassanshahian M, Bayat Z, Saeidi S, et al. Antimicrobial activity of Trachyspermum ammi essential oil against human bacterial. Inter J Adv Biol Biomed Res 2014; 2(1):18-24.

Saeidi S, Shiri Y, Bokaeian M, et al. Antibacterial Activity of essential oil of Saturejah ortensis against multi-drug resistant bacteria. Inter J Enteric Path 2013; 2(2):1-4.

Bokaeian M, Sheikh M, Hassanshahian M, et al. The antibacterial activity of silver nanoparticles produced in the plant Sesamum indicum seed extract: a green method against multi-drug resistant Escherichia coli. Inter J Enteric Path 2014; 2(2):e17928.

Rezaie Keikhaie, K, Ghorbani S, Hosseinzadeh Z, et al. Antimicrobial activity of methanol extract of Citrullus colocynthis against antibiotic-resistant Staphylococcus aureus. Adv Herb Med 2017; 3(3):1-6.

Saeidi S, Amini Boroujeni N, Ahmadi H, et al. Antibacterial activity of some plant extracts against extended- spectrum beta-lactamase producing Escherichia coli isolates. Jundi J Microbiol 2015; 8(2):e15434.

Mohsenipour Z, Hassanshahian M. The inhibitory effect of Thymus vulgaris extracts on the planktonic form and biofilm structures of six human pathogenic bacteria. Avicen J phytomed 2015; 5(4):309-17.

Hassanshahian M, Khosravi F. Study the antimicrobial effects of Artemisia santonica extract on some pathogenic bacteria. Adv Herb Med 2015; 1(4):43-6.

Mohsenipour, Z, Hassanshahian M. Antibacterial activity of Espand (Peganum harmala) alcoholic extracts against six pathogenic bacteria in planktonic and biofilm forms. Biol J Microorgan 2016; 4(16):47-57.

Javadian F, Sepehri Z, Saeedi S, et al. Antifungal effects of the extract of the Withania somnifera on Candida albicans. Adv Her Med 2016; 2(1):32-43.

Sepehri Z, Javadian F, Khammari D, et al. Antifungal effects of the aqueous and ethanolic leaf extracts of Echinophora platyloba and Rosmarinus officinalis. Curr Med Mycol 2016; 2(1):16-25.

Hamayeli H, Shoshtari A, Hassanshahian M, et al. Study the antimicrobial activity of six marine sponges and three parts of sea anemone on Candida albicans. J Coast Life Med 2016; 4(8):122-9.

Mashhadi M, Fakhri J, Saeedi S, et al. Antimicrobial Effects of Medicinal Plants Collected in Zabol, Iran, on Pathogenic Food Pathogenic. J Med Bacteriol 2016; 5(3):18-28

Masoumipour F, Hassanshahian M. Antimicrobial Activity of Five Medicinal Plants on Candida albicans. Iran J Toxicol 2016; 10(6):65-77.

Jahani Z, Hosseinzadeh F, Shahi Z, et al. In vitro study of antimicrobial effects of Rosmarinus officinalis and Glycyrrhiza glabra extracts against some pathogens. Adv Herb Med 2016; 2(4):32-9.

Heydari F, Saeedi S, Hassanshahian M. Antibacterial activity of Mentha longifolia against Salmonella typhimurium. Adv herb Med 2015; 1(3):42-7.

Mohsenipour Z, Hassanshahian M. Investigating the effectiveness of Centaurea cyanus extracts on planktonic growth and biofilm structures of six pathogenic bacteria. SSU J 2014; 22(4):1358-70.

Mohsenipour Z, Hassanshahian M. Inhibitory effects of Tamarix hispida extracts on planktonic form and biofilm formation of six pathogenic bacteria. Biol J Microorgan 2015; 4(13):25-36.

Mohsenipour Z, Hassanshahian M, Moradi M. Investigations of antimicrobial activity of Eucalyptus Camaldulensis extracts against six pathogenic bacteria in planktonic form and biofilm. J Kerman Uni Med Scien 2015; 22(2):172-84.

Sepehri Z, Hassanshahian, M, Shahi Z, et al. Antibacterial effect of ethanol extract of Camellia sinensis l against Escherichia coli. Asian Pac J Microbiol Res 2014; 2(1):6-8.

Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing (2002), 16th International supplement. CLSI document M100-S12. Davis HR. 1997.


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