Antimicrobial Resistance Patterns of Probiotic Strains Isolated from Probiotic Yogurts of Iran

  • Narges Aghajanian Department of Microbiology and Virology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.
  • Mina Gholami Department of Microbiology and Virology, School of medicine, Zanjan University of Medical Sciences, Iran.
  • Mozhgan Kheirandish Department of Microbiology and Virology, School of medicine, Zanjan University of Medical Sciences, Iran.
  • Farzaneh Rafiee Department of Microbiology and Virology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.
  • Davoud Afshar Department of Microbiology and Virology, School of medicine, Zanjan University of Medical Sciences, Iran.
Keywords: Antibiotic resistance, Lactobacillus, Probiotics, Yogurt

Abstract

Background:     Probiotics mainly belong to Lactobacillus spp. and they are useful for humans in appropriate amounts. The present study aimed to determine antibiotic resistance patterns of such bacteria isolated from probiotic yogurts of Iran. Methods:     Probiotic bacteria were isolated from 7 yogurt samples and the isolates were identified by conventional methods and then confirmed using polymerase chain reaction (PCR) technique. Antibiotic susceptibility test was performed by disc diffusion test in order to determine isolates antibiotic resistance patterns. Results:      From 7 yogurt samples, 8 isolates were recovered and PCR assay also showed that the isolates belong to Lactobacillus delbrueckii subsp. bulgaricus. Antibiotic susceptibility testing showed that three isolates from three brands were resistant to vancomycin and gentamicin. Other strains were susceptible to all tested antibiotics. Conclusion:  Low resistance rate to antibiotics among Iranian probiotic bacteria indicate that the consumption of their products is safe but their clearance following antibiotic therapy can be unfavorable. Hence, applying probiotic strains with non-transferrable resistance elements in probiotic yogurts can be a useful strategy to make stable probiotic products.

References

1. Joint F 2002. WHO working group report on drafting guidelines for the evaluation of probiotics in food. London. pp. 30.
2. Klein G, Pack A, Bonaparte C, et al. Taxonomy and physiology of probiotic lactic acid bacteria. Int J Food Microbiol 1998; 41(2):103-25.
3. De Vrese M, Schrezenmeir J, 2008. Probiotics, prebiotics, and synbiotics. Adv Biochem Eng Biotechnol 111:1-66.
4. Gálvez A, Abriouel H, López RL, et al. Bacteriocin-based strategies for food biopreservation. Int J Food Microbiol 2007; 120(1-2):51-70.
5. Dunne C, O'Mahony L, Murphy L, et al. In vitro selection criteria for probiotic bacteria of human origin: correlation with in vivo findings. Am J Clin Nutr 2001; 73(2):386s-92s.
6. Tuomola E, Crittenden R, Playne M, et al. Quality assurance criteria for probiotic bacteria. Am J Clin Nutr 2001; 73(2):393s-8s.
7. Iglesias M, Abadias M, Anguera M, et al. Antagonistic effect of probiotic bacteria against foodborne pathogens on fresh-cut pear. LWT-Food Sci Technol 2017; 81:243-9.
8. Lin PP, Hsieh YM, Tsai CC. Antagonistic activity of Lactobacillus acidophilus RY2 isolated from healthy infancy feces on the growth and adhesion characteristics of entero-aggregative Escherichia coli. Anaerobe 2009; 15(4):122-6.
9. Sharma P, Tomar SK, Goswami P, et al. Antibiotic resistance among commercially available probiotics. Food Res Int 2014; 57:176-95.
10. Zheng M, Zhang R, Tian X, et al. Assessing the risk of probiotic dietary supplements in the context of antibiotic resistance. Front Microbiol 2017; 8:908.
11. Erkkilä S, Petäjä E. Screening of commercial meat starter cultures at low pH and in the presence of bile salts for potential probiotic use. Meat Sci 2000; 55(3):297-300.
12. Koressaar T, Remm M. Enhancements and modifications of primer design program Primer3. Bioinformatics. 2007; 23(10):1289-91.
13. Sanders M, Klaenhammer T. Invited review: the scientific basis of Lactobacillus acidophilus NCFM functionality as a probiotic. J Dairy Sci 2001; 84(2):319-31.
14. Vinderola C, Prosello W, Ghiberto D, et al. Viability of probiotic (Bifidobacterium, Lactobacillus acidophilus and Lactobacillus casei) and nonprobiotic microflora in Argentinian Fresco cheese. J Dairy Sci 2000; 83(9):1905-11.
15. Bernardeau M, Vernoux JP, Henri-Dubernet S, et al. Safety assessment of dairy microorganisms: the Lactobacillus genus. Int J Food Microbiol 2008; 126(3):278-85.
16. D'Aimmo MR, Modesto M, Biavati B. Antibiotic resistance of lactic acid bacteria and Bifidobacterium spp. isolated from dairy and pharmaceutical products. Int J Food Microbiol 2007; 115(1):35-42.
17. Egervärn M, Roos S, Lindmark H. Identification and characterization of antibiotic resistance genes in Lactobacillus reuteri and Lactobacillus plantarum. J Appl Microbiol 2009; 107(5):1658-68.
18. Bonham KS, Wolfe BE, Dutton RJ. Extensive horizontal gene transfer in cheese-associated bacteria. Elife. 2017; 6:e22144.
19. Mathur S, Singh R. Antibiotic resistance in food lactic acid bacteria—a review. Int J Food Microbiol 2005; 105(3):281-95.
20. Salminen S, von Wright A, Morelli L, et al. Demonstration of safety of probiotics—a review. Int J Food Microbiol 1998; 44(1-2):93-106.
21. Gueimonde M, Sánchez B, G de Los Reyes-Gavilán C, et al. Antibiotic resistance in probiotic bacteria. Front Microbiol 2013; 4:202.
Published
2018-10-07
How to Cite
1.
Aghajanian N, Gholami M, Kheirandish M, Rafiee F, Afshar D. Antimicrobial Resistance Patterns of Probiotic Strains Isolated from Probiotic Yogurts of Iran. jmb. 7(3-4):42-8.
Section
Original Articles