Tehran University of Medical Sciences Journal of Medical Bacteriology 2251-8649 1 3-4 2015 10 12 1 9 EN Hajar Mohammadi-Barzelighi Antimicrobial Resistance Research Center, Tehran University of Medical Sciences, Tehran, IR Iran. Mahshid Talebi-Taher Antimicrobial Resistance Research Center, Tehran University of Medical Sciences, Tehran, IR Iran AND Department of Infectious Diseases, Rasoul-e-Akram Hospital, Iran University of Medical Sciences, Tehran, IR Iran. Maryam Adabi Antimicrobial Resistance Research Center, Tehran University of Medical Sciences, Tehran, IR Iran. Seied Ali Javad-Moosavai Department of Internal Medicine, Iran University of Medical Sciences, Tehran, IR Iran. Mosadegh Jabbari Department of Nephrology, Rasoul Akram Hospital, Iran University of Medical Sciences, Tehran, IR Iran. Abdolaziz Rastegar-Lari Antimicrobial Resistance Research Center, Tehran University of Medical Sciences, Tehran, IR Iran AND Department of Microbiology, Iran University of Medical sciences, Tehran, IR Iran. 2015 10 12 2015 10 12 Background: Multi-drug resistant strains of Acinetobacter spp. have created therapeutic problems worldwide. The objective of this study was to detect integrons  in Acinetobacter  spp. isolates  from Ventilator-Associated  Pneu- monia patients using PCR method. Methods: A total 51 Bronchoalveolar lavage samples were obtained from patients in ICU and examined for Acinetobacter spp. infection by biochemical and PCR methods using blaOXA51-like primers. Antimicrobial susceptibility testing was performed using disk diffusion and MIC methods. Results: Among 51 patients with VAP (62.7% males, 35.2% females, mean age 53 year), 50 (98%) were positive, with a high prevalence of gram-nega- tive bacteria, mainly Acinetobacter spp. (70%), from which A. baumani was detected in 34 (68%) and A. lwoffii in 1 (2%) of isolates. More than 90% of isolates were resistant to imipenem,  piperacillin+tazobactam,  third genera- tion cephalosporins and gentamicin, while the most effective antibiotic was colistin (100%). The correlation coefficient between disk diffusion and MIC was 0.808 (p = 0.001). Three Acinetobacter isolates (8%) harbored integrase I gene but none of isolates contained Class II or III integrons. Conclusion: The results showed that colistin was an effective antibiotic and can be used for treatment  of patients in ICU. Due to the high number of MDR isolates lacking Integrons it can be concluded that although class I in- tegrons are important among clinical isolates of A. baumannii, they have no significant  role  in  dissemination  of  antibiotic  resistance  genes  in  Rasoul Akram  Hospital in Tehran, Iran. The presence of IntI in A. lwoffii may be related to transfer of integron to A. baumannii which can be considered as an important threat for hospitalized patients. https://jmb.tums.ac.ir/index.php/jmb/article/view/15 https://jmb.tums.ac.ir/index.php/jmb/article/download/15/12