Tehran University of Medical Sciences Journal of Medical Bacteriology 2251-8649 4 3-4 2015 09 04 30 34 EN Bahareh Lashtoo Aghaee Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. Mohammad Hassan Shirazi Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. Mohammad Reza Pourmand Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. Mostafa Hosseini Department of Statistics School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. Davood Afshar Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. Sara Hajikhani Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. Saeedeh Shobeiri Department of Immunology, Mazandaran University of Medical Sciences, Sari, Iran. 2016 09 13 2016 09 13 Background:  Vibrio species including V. cholera, V. mimicus, V. parahaemolyticus, and V. vulnificus may cause gastrointestinal diseases after seafood consumption, and wound infections in swimmers and fishermen after exposing to seawater. This study determined the prevalence of the four Vibrio species in Tonkabon and Ramsar recreational beach water (approximately 200 meter far from the place where the river reaches the sea) and estuaria (place where the river reaches the sea) in northern part of Iran from autumn 1391 through autumn 1392.   Methods:  Three hundreds water samples were collected for the detection of Vibrio species, using biochemical identification.   Results:   Genomic DNA extracted from isolates and 16Sr DNA PCR confirmed the successful isolation of 9 Vibrio species in recreational beach water region.   Conclusion:  Out of 300 samples, nine positive samples include one V. cholerae and eight V. parahaemolyticus were found at recreational beach (approximately 200 meter far from the place where the river reaches the sea). https://jmb.tums.ac.ir/index.php/jmb/article/view/207 https://jmb.tums.ac.ir/index.php/jmb/article/download/207/152