Tehran University of Medical Sciences Journal of Medical Bacteriology 2251-8649 4 5-6 2015 12 15 13 18 EN Mohammad Mehdi Soltan Dallal Department of Pathobiology, Food Microbiology Division, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. AND Food Microbiology Research Center, Tehran University of Medical Sciences, Tehran, Iran. Enayatollah Kalantar Food Microbiology Research Center, Tehran University of Medical Sciences, Tehran, Iran. AND Dietary Supplements and Probiotic Research Center, Alborz University of Medical Sciences, Karaj, Iran. Laya Kafami Khorasani Department of Microbiology and Immunology, Alborz University of Medical Sciences, Karaj, Iran. Mohammad Hassan Hoshdar Tehrani Medicinal Chemistry Dept. Shahid Beheshti University of Medical Sciences, Tehran, Iran. Monireh Zeinolabedini Zamani Department of Pathobiology, Food Microbiology Division, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. 2017 01 11 2017 01 11 Background: Staphylococcus aureus strains exhibiting multiple antibiotic resistances are isolated from most communities and hospital infections. Treatment of patients with these infections has been difficult. The aim of this study was to detect and extract, the egg yolk immunoglobulin Y as a potential source of anti- S. aureus antibody.  Methods: Specific IgY was produced by immunizing hens with formalin-killed S. aureus. The specificity of serum`s antibody was confirmed by ELISA method. The antibodies were extracted from egg yolk by polyethylene glycol (PEG) precipitation. Proteins were analysed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).  Results: Chicken egg yolk antibodies (IgY) were raised against S. aureus in the serum after injections. Up to 104 dilution specific antibodies were determined in serum.  Conclusion: The results of the ELISA indicates the specificity of the immunoglobulin Y to the target antigen. In order to find a viable alternative to antibiotic treatments, more research must be done on the ability of these antibodies to inhibit the growth of S. aureus. https://jmb.tums.ac.ir/index.php/jmb/article/view/243 https://jmb.tums.ac.ir/index.php/jmb/article/download/243/177