Tehran University of Medical Sciences Journal of Medical Bacteriology 2251-8649 2 1-2 2015 10 12 20 26 EN Enayatollah Kalantar Kurdistan Environmental Health Research Center, Kurdistan University of Medical Sciences, Sanandaj, IR Iran AND Food Supplements and Probiotic Research Center, Alborz University of Medical Sciences, Karaj, IR Iran. Kourush Kabir Food Supplements and Probiotic Research Center, Alborz University of Medical Sciences, Karaj, IR Iran. Fardin Gharibi Deputy of Research, Kurdistan University of Medical Sciences, Sanandaj, IR Iran. Shiva Hatami Food Supplements and Probiotic Research Center, Alborz University of Medical Sciences, Karaj, IR Iran. Afshin Maleki Kurdistan Environmental Health Research Center, Kurdistan University of Medical Sciences, Sanandaj, IR Iran. 2015 10 12 2015 10 12 Background: The current study aimed to determine the effect and properties of surface-modified copper doped Cu:ZnO NPs on killing curves of bacterial pathogens. Methods:  Preparation  of  in  situ  surface-modified  copper  doped  ZnO nanoparticles (Cu:ZnO NPs) was done according to standard procedure.Assay for antimicrobial activity of Cu:ZnO NPs against bacterial pathogens was carried out based on disc diffusion method. Determination of shelf life, thermal and pH stability of antibacterial activity of Cu:ZnO NPs was done and residual activity was determined against the target cultures. Results: FTIR spectra indicate that the nanomaterials synthesized have higher peak intensity compared with reagent grade ZnO. According to the SEM image the nanoparticles synthesized have different size and heterogeneous morphology.  400  ppm  of  Cu:ZnO  NPs  gave  zones  of  inhibition  with diameters of 9.0 – 16 mm against the target cultures. Amongst the target cultures, Escherchia coli was the most sensitive to the Cu:ZnO NPs inhibition zone diameter 16 mm; whereas, 9 mm wide inhibition zone was obtained against  Staphylococcus  aureus. The  Cu:ZnO  NPs  was  fairly stable  for a period of 60 days at room temperature (RT) showing lost of only 20% and 30%     antibacterial  activity  as  tested  against  E.  coli  and  S.  aureus, respectively. The Cu:ZnO NPs was quite stable at this pH and temperature range tested against both E. coli and S. aureus. Conclusion: Surface-modified copper doped Cu:ZnO NPs have significant potential for their usefulness as antibacterial agents. https://jmb.tums.ac.ir/index.php/jmb/article/view/36 https://jmb.tums.ac.ir/index.php/jmb/article/download/36/31