Tehran University of Medical Sciences Journal of Medical Bacteriology 2251-8649 7 1-2 2018 05 20 22 30 EN Reza Akbari Department of Medical Microbiology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Mehdi Fattahi Bafghi Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. Hossain Fazeli Department of Medical Microbiology, Faculty of Medicine, Isfahan University of Medical Sciences, Iran. 2018 05 20 2018 05 20 Background:    Nosocomial infections has always been a major problem for health care and lead to increases in morbidity and mortality in hospitalized patients. The aim of this study was to determine the contamination of personnel and hospital environment and device with nosocomial infections pathogens. Methods:  Wet swab was used to sampling from the hospital personnel and equipment in different wards of a hospital in Isfahan, Iran. Culture and isolation of strains were done according to the standard microbial methods. Identification was based on gram staining, microbial and biochemical tests.   Results:  Overall 236 samples; 123 and 113 samples were collected from 91 hospital personnel and hospital environment and devices respectively. S. aureus was isolated from 14.2%, 15.3% and 5.4% of personnel hands, uniform and throat respectively. The cases of Enterobacteriaceae were isolated from personnel hands, uniform and throat as 13.9%, 4.3% and 3.27% respectively. P. aeruginosa was isolated from personnel hands (4.3%), uniform (6.5%) and throat (6.5%). Conclusion:   S. aureus, Enterobacteriaceae and P. aeruginosa were the most prevalence bacteria that isolated from the personnel hands, uniforms, throats and hospital environment and devices. Personnel hands were the most contaminated part considering both the numbers and diversity in isolated organisms. Contamination of the hospital personnel and hospital environment and devices have a main role in dispersion of the nosocomial pathogens in hospital environment. With a good hygienic administration and an excellent healthy management planning in hospitals can be inhibit the dispersion of nosocomial pathogens in hospitals environment.  Hospital environment and devices, Hospital personnel, Microbial Pathogens, Nosocomial infections. https://jmb.tums.ac.ir/index.php/jmb/article/view/372 https://jmb.tums.ac.ir/index.php/jmb/article/download/372/255