Tehran University of Medical Sciences Journal of Medical Bacteriology 2251-8649 1 1-2 2015 10 12 3 8 EN Hossein Goudarzi Department of Medical Microbiology, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran. Hanieh Rezaee Department of Medical Microbiology, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran. Mitra Rafizadeh Department of Pathology, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran. Afsoon Taghavi Department of Medical Microbiology, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran AND Department of Pathology, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran. 2015 10 12 2015 10 12 Background: Many  Helicobacter pylori strains express adhesin proteins that bind to specific  host-cell  macromolecule  receptors,  like sialic acid binding adhesion (sabA).   SabA-expressing  strains have been associated with gastric cancer  and  negatively  associated   with  duodenal   ulcers. The  aim  of  this study  was  to  determine   the  status  of  sabA  gene  of  H.  pylori   and  its association  with the clinical diseases in Iranian dyspeptic pateints. Methods:  Eighty six biopsy block samples  that were positive  for H. pylori according Geimsa staining were included in  this study. Genomic DNA was extracted  from paraffin-embedded  gastric biopsies  obtained  from dyspeptic patients.     The     identity     of     Helicobacter      genus     was     determined through amplification  of 16S rRNA which followed  by sabA PCR using the gene-specific primers.  The prevalence  of sabA gene in three clinical groups including gastritis, gastric ulcer, and gastric atrophy was determined. The association  of  sabA  gene  and  clinical  outcomes  was  assessed  statistically using Chi-square  test. A p-value less than <0.05 was considered  statistically significant. Results:  Total  of 86 patients  was  included  in this study.  Seventeen  cases out  of  86  (23.6%)   were  yielded   a  positive   result  for  sabA  gene.  The prevalence  of sabA   gene   was   28.6%   in   both   dyspeptic   and   Gastric atrophy  patients  as compared with peptic ulcers (19.2%). Conclusion:   For  a  first  time  the  frequency   of  sabA   gene  using  PCR methods  was reported.  The current  study demonstrated  that the sabA  gene status was not associated with clinical diseases. In limited number of studied samples,  higher  frequency  of  sabA  gene  among  dyspeptic   and  atrophic patients was found.   https://jmb.tums.ac.ir/index.php/jmb/article/view/4 https://jmb.tums.ac.ir/index.php/jmb/article/download/4/2