Tehran University of Medical Sciences Journal of Medical Bacteriology 2251-8649 8 5-6 2019 09 08 38 43 Mohammad Mehdi Soltan Dallal Division of Food Microbiology, Department of pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. AND Food Microbiology Research Center, Tehran University of Medical Sciences, Tehran, Iran. Monireh Zeinolabedini Zamani Food Microbiology Research Center, Tehran University of Medical Sciences, Tehran, Iran. Laya Kafami Khorasani Dietary Supplements and Probiotic Research Center, Alborz University of Medical Sciences, Karaj, Iran. Mohammad Hassan Houshdar Tehrani Medicinal Chemistry Department, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Mohammad Kazem Sharifi Yazdi Zoonosis Research Center, Department of Medical Laboratory Sciences, Tehran University of Medical Sciences, Tehran, Iran. Saeid Vahedi Instructor of Department of anesthesia, School of Allied Medicine, Tehran University of Medical Sciences, Tehran, Iran. Farnaz Soltani Jonaghani Food Microbiology Research Center, Tehran University of Medical Sciences, Tehran, Iran. Fatemehsadat Mirsane Food Microbiology Research Center, Tehran University of Medical Sciences, Tehran, Iran. 2019 02 26 2019 03 09 Background: The aim of this study was to provide a simple and efficient way of producing IgY antibodies against Escherichia coli and extract that by polyethylene glycol (PEG) precipitation method. Methods: An ti- Escherichia coli antibody was produced in hens by using formalin-killed E.coli and confirmed by ELISA method, applying serum`antibody. The specific IgY was extracted from egg yolk by Polyethylene Glycol (PEG) Precipitation and analysed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Results: The result of ELISA shows that the IgY titre increased from initial immunization and the high titre (≥0.071) persisted after the first immunization. Heavy chain of IgY with ~68 KDa was observed in the Gel electrophoresis pattern. Conclusion: The results of the ELISA indicates the specificity of the immunoglobulin Y to the target antigen and the result of SDS-PAGE represented the appropriate extraction method. More research must be done on the ability of these antibodies to inhibit the growth of E.coli. https://jmb.tums.ac.ir/index.php/jmb/article/view/411 https://jmb.tums.ac.ir/index.php/jmb/article/download/411/299