Tehran University of Medical Sciences Journal of Medical Bacteriology 2251-8649 2 3-4 2015 10 12 11 16 EN Neda Noori Department of Microbiology, College of Basic Sciences, Karaj Branch, Islamic Azad University, Alborz, IR Iran. Jalil Vandyosefi Department of Microbiology, College of Basic Sciences, Karaj Branch, Islamic Azad University, Alborz, IR Iran. Faeze Sabet Antimicrobial Resistance Research Center, Buali Research Institute, Department of Microbiology and Virology, Medical School, Mashhad University of Medical Sciences, Mashhad, IR Iran. Sara Ashrafi Antimicrobial Resistance Research Center, Buali Research Institute, Department of Microbiology and Virology, Medical School, Mashhad University of Medical Sciences, Mashhad, IR Iran. Kiarash Ghazvini Antimicrobial Resistance Research Center, Buali Research Institute, Department of Microbiology and Virology, Medical School, Mashhad University of Medical Sciences, Mashhad, IR Iran. 2015 10 12 2015 10 12 Background: The emergence and rapid spread of metallo-beta-lactamase (MBL) producing Acinetobacter spp. are of great concern worldwide due to limited treatment options. Epidemiologic studies of the causing genes are important for prevention. Methods:  In  this  study,  70  imipenem-resistant  Acinetobacter  strains  were isolated from health care associated infections. These isolates were screened for detection of metallo-beta-lactamase (MBL) using inhibitor potentiated disk diffusion tests with thylenediaminetetraacetic acid (EDTA). PCR was designed for detection of bla vim and bla imp-1 using specific primers. Results: Among these 70 strains, 50 strains appeared to produce metallo-beta-lactamase. Three isolates were detected by PCR to carry metallo-beta-lactamase gene bla vim, but bla imp-1 gene was not detected. Conclusion: These findings suggest that in our area other genetic elements are responsible for resistance against metallo-beta-lactams. https://jmb.tums.ac.ir/index.php/jmb/article/view/46 https://jmb.tums.ac.ir/index.php/jmb/article/download/46/41