Tehran University of Medical Sciences Journal of Medical Bacteriology 2251-8649 12 3 2024 08 21 9 17 Behnaz Montazeri MSc, Islamic Azad University, Chalus Branch, Department of medical science, Chalous, Iran Haniyeh Bashi Zadeh Fakhar Department of Human Geneticse, Science And Research Branch, Branch, Islamic Azad University, Tehran, Iran Babak Shaghaghi Department of Laboratory Science, Chalous Branch, Islamic Azad University, Chalous, Iran Forouzan Rostami Assisstant Professor,Department of Nursing, Faculty of Nursing and Midwifery, Islamic Azad University, Chalous Branch, Chalous,Iran 2024 02 15 2024 06 22 Background:   Herpes simplex type 2 is a common infection worldwide. This disease is common in both developed and developing countries. Early detection of infection is very important to reduce the risk of infection. Real-time reliable PCR is a very sensitive and specific method that can be used as the best marker in determining the therapeutic effect by identifying a viral genome in an individual. The prevalence of herpes simplex virus type 2 in women with genital herpes was evaluated by Real time PCR method. Methods:   From January 1999 to March 2010, 45 samples of vaginal swab s and cervix of women with genital herpes were examined for HSV virus DNA detection using Real Time PCR. Results:   The mean age of the patients was 35.9 + 5.9. The percentage of positive cases of herpes simplex virus type 2 in the studied women was 22.2% and the history of infection with hpv was 33.3% vs. 12.5%. = 0.094 which was significant. https://jmb.tums.ac.ir/index.php/jmb/article/view/515