Tehran University of Medical Sciences Journal of Medical Bacteriology 2251-8649 12 3 2024 08 22 62 67 Rachida Issa Moussa Faculty of Medicine and Pharmacy Mohamed V University Rabat Bacteriology, Mohamed V Military Training Hospital, Rabat, Morocco Ikram Habboubat Faculty of Medicine and Pharmacy, Mohamed V University, Rabat Bacteriology, Mohamed V Military Training Hospital, Rabat, Morocco Yassine Ben Lahlou Faculty of Medicine and Pharmacy Mohamed V University Rabat Bacteriology, Mohamed V Military Training Hospital, Rabat, Morocco Mariama Chadli Faculty of Medicine and Pharmacy, Mohamed V University, Rabat Bacteriology, Mohamed V Military Training Hospital, Rabat, Morocco 2024 03 10 2024 04 07 Background:   The various species of the Listeria genus are frequently isolated from food and drink products. They are classified into two categories according to their pathogenicity. Pathogenic species are frequently incriminated in infections of the elderly, immunocompromised and pregnant women. The Listeria grayi species is rarely isolated as a pathogen in human pathologies. We report a case of L. grayi meningitis in a polypathological patient. Antimicrobial susceptibility testing revealed resistance to ampicillin and susceptibility to Meropenem. Therapeutic failure was recorded despite in vitro sensitivity. Methods:   Conventional biochemical test and Api Listeria gallery was used for identification of the isolate. Results:   We report a case of L. grayi meningitis in a polypathological patient.  Antimicrobial susceptibility testing revealed resistance to ampicillin and susceptibility to Meropenem. Therapeutic failure was recorded despite in vitro sensitivity. Conclusion:   Given its infrequent reporting, Listeria grayi meningitis warrants consideration, especially in elderly patients with multiple pathologies.  https://jmb.tums.ac.ir/index.php/jmb/article/view/517