Tehran University of Medical Sciences Journal of Medical Bacteriology 2251-8649 13 2 2025 05 06 79 84 Oves Siddiqui Department of Microbiology, Maulana Azad Medical College & Lok Nayak Hospital, New Delhi, India. Ashish William Department of Microbiology, Maulana Azad Medical College & Lok Nayak Hospital, New Delhi, India. Nilakshi Gupta Department of Microbiology, Maulana Azad Medical College & Lok Nayak Hospital, New Delhi, India. Swati Nirmal Department of Microbiology, Maulana Azad Medical College & Lok Nayak Hospital, New Delhi, India. Sonal Saxena Department of Microbiology, Maulana Azad Medical College & Lok Nayak Hospital, New Delhi, India. Prabhav Aggarwal Department of Microbiology, Maulana Azad Medical College & Lok Nayak Hospital, New Delhi, India. 2025 01 09 2025 02 06 Background:   Bacteroides fragilis is a clinically significant anaerobic bacterium known to cause abdominal infections, abscesses, sepsis, and skin and soft tissue infections. We report a case of an intra-abdominal wound infection following a penetrating injury, attributed to B. fragilis. Methods:   Foul-smelling pus from the abdominal wound was collected and sent for anaerobic culture using Robertson’s cooked meat medium. Gram staining and culture were performed on blood agar supplemented with haemin and vitamin K1. The sample's gram stain revealed gram-negative bacilli. Results:   Colony growth was observed on blood agar after 48 hours and was identified as B. fragilis through phenotypic biochemical tests, MALDI-TOF MS, and real-time PCR. The patient was successfully managed with metronidazole. Conclusion:  Prompt diagnosis and early treatment were crucial in reducing the morbidity and mortality associated with this infection. https://jmb.tums.ac.ir/index.php/jmb/article/view/557