Tehran University of Medical Sciences Journal of Medical Bacteriology 2251-8649 13 3 2025 08 24 115 117 Hamid Sadeghi 1 Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran. 2 Student Research Committee, Qazvin University of Medical Sciences, Qazvin, Iran. Farhad Nikkhahi Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran. Susan Khanjani Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran. Saeideh Gholamzadeh Khoei Cellular and Molecular Research Center, Research Institute for Prevention of Non-Communicable Diseases, Qazvin University of Medical Sciences, Qazvin, Iran. Mohammad Maleki Clinical Research Development Unit, Kowsar Hospital, Qazvin University of Medical Sciences, Qazvin, Iran. Masoumeh Aslanimehr Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran. 2025 03 28 2025 04 28 Vancomycin is an essential glycopeptide antibiotic facing challenges from resistant bacterial strains such as vancomycin-intermediate Staphylococcus aureus (VISA) and heterogeneous VISA (hVISA). Timely and precise identification of these strains is vital for efficient infection control. This report explores using aptamer-based biosensors as a novel solution for the rapid detection of hVISA. Aptamers, short, single-stranded nucleic acids, exhibit remarkable specificity and strong affinity for their targets, making them outstanding candidates for biosensor creation. These biosensors offer affordability, ease of customization, and swift performance, making them highly appropriate for medical settings. By utilizing electrochemical detection techniques, aptamer-based biosensors facilitate real-time tracking of vancomycin concentrations and may also contribute to the efficient detection of hVISA, addressing urgent demands in infectious disease control. https://jmb.tums.ac.ir/index.php/jmb/article/view/569