Tehran University of Medical Sciences Journal of Medical Bacteriology 2251-8649 1 1-2 2015 10 12 31 37 EN Mohammad Mehdi Soltan-Dallal Division of Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, IRIran AND Antibiotic Resistant Research Center, Tehran University of Medical Sciences, Tehran, IR Iran. Farhad Nikkhahi Division of Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran. Saber Molaei Eye Research Center, Farabi Eye Hospital, Tehran, IR Iran. Kazem Hosseini Quality Control Manager of Iranian Tissue Bank Research & Preparation Center, Stem Cells Preparation Unit., Tehran, IR Iran. Zohreh Kalafi Division of Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran. Ahad khoshzaban Eye Research Center, Farabi Eye Hospital, Tehran, IR Iran. Abdolaziz Rastegar-Lari Antibiotic Resistant Research Center, Tehran University of Medical Sciences, Tehran, IR Iran. Ahmad Kheirkhah Eye Research Center, Farabi Eye Hospital, Tehran, IR Iran AND Quality Control Manager of Iranian Tissue Bank Research & Preparation Center, Stem Cells Preparation Unit., Tehran, IR Iran. Mohammad Kazem Sharifi-Yazdi Department of Medical Laboratory Sciences, School of Paramedicene, Tehran University of Medical Sciences, Tehran, IR Iran. 2015 10 12 2015 10 12 Background:  Keratitis caused by Pseudomonas  aeruginosa  is often resulted in  severe  corneal  ulcers  and  perforation,  which  leads  to  losses  of  vision. Human amniotic membrane (HAM) forms the inner wall of the membranous sac which surrounds and protects the embryo during gestation. The purpose of this  study  was  to  evaluate  the  effectiveness  of  the  amniotic  membrane's healing in rabbits with pseudomonas keratitis. Methods:  In total 14 rabbits divided in 2 groups of: 1 as Control  and 2 as experimental  amniotic  membrane  combined  with  ciprofloxacin.  A 0.05  ml suspension   of  Pseudomonas   aeruginosa   ATCC  27853  was  injected  into rabbit’s corneal stroma, with no interference in control group. In the second group, the amniotic membrane in pieces of 1.5 × 1.5 cm transplanted  to the entire corneal surface by eight interrupted 10.0 nylon sutures. In the first day ciprofloxacin  drop was injected  to the second  group  every 30 minutes  and through second to seventh days every 2 hours. The results of perforation  in cornea and the amount of infiltration were registered. Results:  The  results  showed  that  amniotic  membrane  transplantation (AMT)  +  ciprofloxacin  group  had  0%  perforation  and  the  control  group 85.6%. Average infiltrations were 5 mm in AMT + ciprofloxacin groups and 23.75 mm in control. Conclusion: The use of amniotic membrane with ciprofloxacin was effective in   prevention   of   cornea   perforation   and   controlling   the   process   of pseudomonal  keratitis remission.  The improvement  of inflammation  rapidly happened in ciprofloxacin + AMT group.   https://jmb.tums.ac.ir/index.php/jmb/article/view/9 https://jmb.tums.ac.ir/index.php/jmb/article/download/9/7