Journal of Medical Bacteriology 2015. 4(5, 6):24-30.

Anti-CagA IgG Antibody Is Independent from Helicobacter pylori VacA and CagA Genotypes
Hashem Fakhre Yaseri, Mehdi Shakaraby, Hamid Reza Baradaran, Seyed Kamran Soltani Arabshhi

Abstract


Background: Helicobacter pylori strains have two classical virulence genes, the cytotoxin-associated A (cagA) gene and the vacuolating cytotoxin A (vacA) gene, which are located in the cag pathogenicity island (cagPAI). Serum immunoglobulin G (IgG) antibodies to H. pylori, especially, the CagA antigen may be a reliable marker for selection of dyspeptic patients for upper endoscopy.

 Methods: Serum sample of 129 dyspeptic patients with positive H. pylori, were tested for serum IgG Anti-CagA antibody by ELISA. The presence of the cagA and vacA genotypes were determined using polymerase chain reaction (PCR) on biopsy samples taken via endoscopy.

 Results: Positive serum IgG anti-CagA antibodies in patients with cagA+/vacA+ and cagA+/vacA-genotypes were 22/23 (95.6%) and 18/19 (94.7%), respectively. In addition, serum IgG anti-CagA antibodies in patients with cagA-/vacA+ and cagA-/vacA- genotypes were 22/47 (46.8%) and 33/40 (82.5%), respectively.

 Conclusion: It can be concluded that the serum IgG anti-CagA antibody alone could select patients with dyspepsia following upper endoscopy. The assessment of vacuolating cytotoxin activity of H. Pylori is, therefore, not required, even when vacA gene is positive. This hypothesis needs to be studied in a large number of patients with dyspepsia.


Keywords


Dyspepsi, CagA, VacA.

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References


Fock KM and Ang TL. Epidemiology of Helicobacter pylori infection and gastric cancer in Asia. Gastroentrol and Hepatol 2010; 25: 479-86.

Suzuki T, Matsuo K, Ito H, et.al. A past history of gastric ulcers and Helicobacter pylori infection increase the risk of gastric malignant lymphoma. Carcinogenesis 2006; 27(7): 1391-7.

Atherton JC, Blaser MJ. Coadaptation of

Helicobacter pylori and humans: ancient history modern implications. J Clin Investig 2009; 119: 2475-87.

Costa DM, Pereira ES and Rabenhorst SHB. What exists beyond cagA and vacA? Helicobacter pylori genes in gastric diseases. World J Gastroenterol 2015; 21 (37): 10563-72.

Blaser MJ, Atherton JC. Helicobacter pylori persistence: biology and disease. J Clinical Investig 2004; 113: 321-33

Sgouras DN, Trang TTH and Yamaoka Y. Pathogenesis of Helicobacter pylori Infection. Helicobacter 2015; 20 (Suppl. 1): 8-16.

Kumar S, Kumar A and Kumar Dixit V. Direct detection and analysis of vacA genotypes and cagA gene of Helicobacter pylori from gastric biopsies by novel multiplex polymerase chain reaction assay.

Dia Microbiol and Infect Dis 2008; 62: 366-73.

Basso D, Zambon CF, Letley DP, et.al. Clinical Relevance of Helicobacter pylori cagA and vacA genes Polymorphisms.

Gastroentrol 2008; 135: 91-9.

Amsterdam KV, Van Vliet AHM, Kusters JG, et.al. Of microbe and man: determinants of Helicobacter pylori-related diseases.

FEMS Microbiol Rev 2006; 30 (1): 131-56.

Ernst PB, Peura DA, and Crowe SE. The translation of Helicobacter pylori Basic Research to Patient Care. Gastroentrol 2006; 130: 188-206.

Bridge DR and Merrell DS. Polymorphism in the Helicobacter pylori CagA and VacA toxins and disease. Gut Microb 2013; 4(2): 101-17.

Lepper PM, Moricke A, Vogt K, et.al. Comprison of different criteria for interpretation of immunoglobulin G immunobloatting results for diagnosis of Helicobacter pylori infection. Clin Dia Lab Immun 2004; 11: 569-76.

Moayyedi P, Duffett S, Braunhottz D, et.al. The Leed´s Dyspepsia Questioner (LDQ); a valid tool for measuring the presence and severity of dyspepsia. Alimen Pharmacol Ther 1998; 12: 1257-62.

Mukhopadhyay AK, Kersulyte D, Yong Jeong J, et.al. Distinctiveness of Genotypes of Helicobacter pylori in Calcutta, India. J Bacteriol 2000; 182(11): 3219-27.

Wroblewski LE, Peek RM and Wilson KT. Helicobacter pylori and Gastric Cancer: Factors That Modulate Disease Risk. Clin Microbiol Rev 2010; 23(4): 713-39

Módena JLP, Sales AIL, Acrani GO, et.al. Association between Helicobacter pylori genotypes and gastric disordersin relation to the cag pathogenicity island. Dia Microbiol and Infect Dis 2007; 59: 7-16

Calvet X, Lazaro MJR, Lehours PH, et.al. Diagnosis and Epidemiology of Helicobacter pylori Infection. Helicobacter 2013; 18(Suppl.1): 5-11.


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