An In Vitro Assessment of Antimicrobial and Cytotoxic Effects of Nanosilver
AbstractBackground: The antimicrobial activity of silver nanoparticles has been investigated in medical fields in recent years, but there are few studies regarding its effect on oral microorganisms. The aim of the present study was to evaluate the in vitro antimicrobial and toxicity properties of nanosilver against two dental plaque microorganisms and Human Gingival Fibroblast (HGF) cell line.Methods: Antibacterial effects of nanosilver colloidal solution were determined by minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) using microdilution method. Standard strains of Streptococcus sanguis and Actinomyces viscosus were used. For toxicity assessment, MTT and LDH tests were performed under con- trolled conditions. Different concentrations of nanosilver were prepared and their toxic effects on HGF were determined after 24, 48 and 72 hours.Results: The MIC of nanosilver solution for S. sanguis and A. viscosus were 16 and 4 µ g/ml, respectively. The MBC of nanosilver was 64 µ g/ml for S. sanguis and 16 µ g/ml for A. viscosus. MTT results showed that after 24 hours the concentrations of ≥ 0.5 µ g/ml of nanosilver solution affected cell viability when compared with control group. After 48 and 72 hours only the concentration of ≥ 5 µ g/ml showed significant effect on cultured cell viability. LDH release test demonstrated toxic effect only after 48, 72 hours by 20 and 50 µ g/ml of nanosilver.Conclusion: The results demonstrated that beside its antibacterial activity against S. sanguis and A. viscosus, nanosilver mediated a concentration and time dependent cytotoxicity on HGF.
Newman MG, Takei HH, Klokkevold PR, Carranza FA, 2011. Clinical Pe- riodontology, 11th ed. Philadelphia.
Haffajee A, Yaskell T, Socransky S.Antimicrobial effectiveness of an her- bal mouthrinse compared with an es- sential oil and a chlorhexidine mouth- rinse. JADA 2008; 139 (5): 606-11.
Alt V, Bechert T, Steinrücke P, et al.An in vitro assessment of the antibac- terial properties and cytotoxicity of nanoparticulate silver bone cement. Biomaterials 2004; 25 (18): 4383-91.
Kim JS, Kuk E, Yu KN, et al. Antim- icrobial effects of silver nanoparticles. Nanomedicine J 2007; 3 (1): 95-101.
Kim H, Kang H, Chu G. Antifungal ef- fectiveness of nanosilver colloid against rose powdery mildew in greenhouses. Solid State Phenomena J 2008; 135 (1):15-18.
Lara H, Ayala-Nuñez L, Ixtepan-Tur- rent L, et al. Mode of antiviral action of silver nanoparticles against HIV-1. J Nanobiotech 2010; 8 (1): 1186-1477.
Lu L, Sun RW, Chen R, et al. Silver nanoparticles inhibit hepatitis B virus replication. AntivirTher J 2008; 13 (2):253-62.
Sun L, Singh AK, Vig K. Silver Na- noparticles Inhibit Replication of Respiratory Syncytial Virus. J Bio- med Biotechnol 2008; 4 (2): 149-58.
Gaiser BK, Fernandes TF, Jepson M, et al. Assessing exposure, uptake and toxicity of silver and cerium dioxide nanoparticles from contaminated en- vironments. Environ Health 2009;21: 8 Suppl 1:S2.
Lok CN, Ho CM, Chen R, et al. Pro- teomic analysis of the mode of anti- bacterial action of silver nanoparticles. J Proteome Res 2006; 5 (4): 916- 24.
Nel A, Xia T, Mädler L, et al. Toxic potential of materials at the nanole- vel. Science 2006; 311 (5761): 622.
Motskin M, Wright DM, Muller K, et al. Hydroxyapatitenano and micro- particles: Correlation of particle prop- erties with cytotoxicity and biostability. Biomaterials 2009; 30 (19): 3307-17.
Stratmeyer ME, Goering PL, Hitchins VM, et al. What we know and don't know about the bioeffects of nanoparti- cles: developing experimental ap- proaches for safety assessment. Biomed Microdevices 2010; 12 (4): 569-73.
Miura N, ShinoharaY. Cytotoxic effect and apoptosis induction by silver nano- particles in HeLa cells. BiochemBiophys Res Commun 2009; 390 (3): 733-7.
Hussain SM, Hess KL, Geahart JM, et al. In vitro toxicity of nano particles in BRL rat liver cells. Toxicol in vitro 2005; 19: 975- 983.
Chae YJ, Pham CH, Lee J, et al. Eval- uation of the toxic impact of silver na- noparticles on Japanese medaka (Oryzias latipes). Aquat Toxicol 2009;94 (4): 320-7.
Shavandi Z, Ghazanfari T, Moghad- dam KN. In vitro toxicity of silver na- noparticles on murine peritoneal mac- rophages. Immunopharmacol Immunotoxicol 2011; 33 (1): 135-40.
Hsin YH, Chen CF, Huang S, et al.The apoptotic effect of nanosilver is mediated by a ROS- and JNK-de- pendent mechanism involving the mi- tochondrial pathway in NIH3T3 cells. Toxicol Lett 2008; 179 (3): 130-9.
Liao J, Anchun M, Zhu Z, et al. Anti- bacterial titanium plate deposited by- silver nanoparticles exhibits cell com- patibility. Int J Nanomedicine 2010; 5:337-42.
Lindhe J, Lang NP, Karring T, 2006.Clinical Periodontology and Implant Dentistry, 5th ed. Munksgaard.
Ross MH, Pawlina W, 2006. Histol- ogy: a text and atlas, 5th ed. Lippincott Williams & Wilkins, Philadelphia.
Baron EJ, Finegold SM, 1990. Bailey and scott’s Diagnostic Microbiology,8th ed. mosby company, Missouri.
Wikler MA, Cockerill FR, Craig WA, et al. Methods for Dilution Antimicr obial Susceptibility Tests for Bacteria That Grow Aerobically. Approved St andard J 2009; 29 (2): 552-8.
Gazi MI, Davies TJ, al-Bagieh N, et al. The Immediate and Medium-Term Effects of Meswak on the composi- tion of mixed saliva. J Clin Periodontol 1992; 19 (2): 113.
Shahoon H, Hamedi R, Golgonia P, et al. Evaluation of nanosilver particles’ cytotoxicity on L929 fibroblast cells by MTA assay: an in vitro study. J Res in Dent Sci 2011; 8 (2): 53-9.
Cao XL, Cheng C, Ma YL, et al. Prep- aration of silver nanoparticles wi th an- timicrobial activities and the researches of their biocompatibilities. J Mater Sci Mater Med 2010; 21 (10): 2861-8.