Evaluation of the Antibacterial Effects of Nickel Nanoparticles on Biofilm Production by Streptococcus mutans
AbstractBackground: Dental caries is a biofilm-dependent disease mainly causes by cariogenic bacteria that colonize dental surfaces, especially Streptococcus mutans. Nickel is a safe metal element which routinely used in dental compounds. The antibacterial and anti-biofilm effects of nickel nanoparticles (Ni-NPs) have been determined in limited studies, but the anti-biofilm effects of Ni-Nps on S. mutans have not been investigated before, so this study was aimed to investigate the anti-biofilm effects of Ni-NPs on S. mutans ATCC 35668.Methods: Biofilm formation by S. mutans ATCC 35668 was assayed by Microtiter Dish Biofilm Formation Assay and absorbance was measured by ELISA reader at 550 nm. The amounts of biofilm formation were also measured in the presence of 1, 01 and 0.01mg/mL concentrations of Ni- NPs by the same protocol and the mean amounts were compared between groups. Eight replicates were considered for each experiment. Data was statistically analyzed by SPSS16 software.Results: According to the statistical analysis, the amounts of biofilm formation were significantly reduced in the presence of all the tested concentrations of Ni-NPs.Conclusion: The current findings showed the potent anti-biofilm effects of Ni-NPs even in a concentration as low as 0.01 mg/ mL, so it is proposed for different applications in dentistry, considering its anti-biofilm effects. However, further studies
Legenova KBujdakova H. The role of Streptococcus mutans in the oral biofilm. Epidemiol Mikrobiol Imunol 2015; 64(4): 179-87.
Marcenes W, Kassebaum NJ, Bernabe E, et al. Global burden of oral conditions in 1990-2010: a systematic analysis. J Dent Res 2013; 92(7): 592-7.
Branda SS, Vik S, Friedman L, et al. Biofilms: the matrix revisited. Trends Microbiol 2005; 13(1): 20-6
Flemming HC and Wingender J. The biofilm matrix. Nat Rev Microbiol 2010; 8(9): 623-33.
Venkatesan N, Perumal G, Doble M. Bacterial resistance in biofilm-associated bacteria. Future Microbiol 2015; 10(11): 1743-50.
Arthur RA, Cury AA, Graner RO, et al. Genotypic and phenotypic analysis of S. mutans isolated from dental biofilms formed in vivo under high cariogenic conditions. Braz Dent J 2011; 22(4):267-74.
Liu R, Memarzadeh K, Chang B, et al. Antibacterial effect of copper-bearing titanium alloy (Ti-Cu) against Streptococcus mutans and Porphyromonas gingivalis. Sci Rep 2016; 6: 29985.
Szunerits S, Barras A, Boukherroub R Antibacterial applications of nanodiamonds. Int J Environ Res Public Health 2016; 13(4): 413.
Nagaich U and Gulati NChauhan S. Antioxidant and antibacterial potential of silver nanoparticles: biogenic synthesis utilizing apple extract. J Pharm (Cairo) 2016: 7141523.
Dhanyalayam D, Scrivano L, Parisi OI, et al. Biopolymeric self-assembled nanoparticles for enhanced antibacterial activity of Ag-based compounds. Int J Pharm 2016; 517(1-2): 395-402.
Patil MP, Kim GD. Eco-friendly approach for nanoparticles synthesis and mechanism behind antibacterial activity of silver and anticancer activity of gold nanoparticles. Appl Microbiol Biotechnol 2016; 101(1): 79-92.
Gopinath K, Kumaraguru S, Bhakyaraj K, et al. Green synthesis of silver, gold and silver/gold bimetallic nanoparticles using the Gloriosa superba leaf extract and their antibacterial and antibiofilm activities. Microb Pathog 2016; 101: 1-11.
Essa A and MKhallaf MK. Antimicrobial potential of consolidation polymers loaded with biological copper nanoparticles. BMC Microbiol 2016; 16(1): 144.
Choi HJ, Choi JS, Park BJ, et al. Enhanced transparency, mechanical durability, and antibacterial activity of zinc nanoparticles on glass substrate. Sci Rep 2014; 4: 6271.
Mu H, Tang J, Liu Q, et al. Potent antibacterial nanoparticles against biofilm and intracellular bacteria. Sci Rep 2016; 6: 18877.
Argueta-Figueroa L, Morales-Luckie RA, Scougall-Vilchis RJ, et al. Synthesis, characterization and antibacterial activity of copper, nickel and bimetallic Cu–Ni nanoparticles for potential use in dental materials. Prog Nat Sci 2014; 24(4): 321-8.
Coates A, Hu Y, Bax R, et al. The future challenges facing the development of new antimicrobial drugs. Nat Rev Drug Discov 2002; 1(11): 895-910.
Goransson H, Molander A, Karlsson J, et al. The adoption of nickel-titanium rotary instrumentation increases root-filling quality amongst a group of Swedish general dental practitioners. Swed Dent J 2014; 38(1): 15-22.
Raap U, Stiesch M, Reh H, et al. Investigation of contact allergy to dental metals in 206 patients. Contact Dermatitis 2009; 60(6): 339-43.
O'Toole GA. Microtiter dish biofilm formation assay. J Vis Exp 2011; 47. pii: 2437.
Stepanovic S, Vukovic D, Hola V, et al. Quantification of biofilm in microtiter plates: overview of testing conditions and practical recommendations for assessment of biofilm production by staphylococci. APMIS 2007; 115(8): 891-9.
Kalishwaralal K, BarathManiKanth S, Pandian SR, et al. Silver nanoparticles impede the biofilm formation by Pseudomonas aeruginosa and Staphylococcus epidermidis. Colloids Surf B Biointerfaces 2010; 79(2):340-4.
Bass JK, Fine H, Cisneros GJ. Nickel hypersensitivity in the orthodontic patient. Am J Orthod Dentofacial Orthop 1993; 103(3): 280-5.
Kulkarni P, Agrawal S, Bansal A, et al. Assessment of nickel release from various dental appliances used routinely in pediatric dentistry. Indian J Dent 2016; 7(2): 81-5
Peltonen L. Nickel sensitivity in the general population. Contact Dermatitis 1979; 5(1): 27-32.
Bhaskar V and Subba Reddy VV. Biodegradation of nickel and chromium from space maintainers: an in vitro study. J Indian Soc Pedod Prev Dent 2010; 28(1): 6-12.
Kuramitsu HK. Characterization of extracellular glucosyltransferase activity of Steptococcus mutans. Infect Immun 1975; 12(4): 738-49.
Baker JL, Faustoferri R, CQuivey RG, Jr. Acid-adaptive mechanisms of Streptococcus mutans-the more we know, the more we don't. Mol Oral Microbiol 2016; 32(2): 107-17.
Choi HJ, Choi JS, Park BJ, et al. Enhanced transparency, mechanical durability, and antibacterial activity of zinc nanoparticles on glass substrate. Sci Rep (2014); 4: 6271.
Aldujaili NH, Abdullah NY, Khaqani RL. Biosynthesis and antibacterial activity of titanium nanoparticles using Lactobacillus. Int J Recent Sci Res 2015; 6(12): 7741-51.
Mamonova I. Study of the antibacterial action of metal nanoparticles on clinical strains of gram negative bacteria. World J Med Sci 2013; 8(4): 314.
Ashtari K, Fasihi J, Mollania N, et al. A biotemplated nickel nanostructure: Synthesis, characterization and antibacterial activity. Mater Res Bull 2014; 50: 348-353.
Shi SF, Jia JF, Guo XK, et al. Reduced Staphylococcus aureus biofilm formation in the presence of chitosan-coated iron oxide nanoparticles. Int J Nanomedicine 2016; 11: 6499-506.
Lara HH, Romero-Urbina DG, Pierce C, et al. Effect of silver nanoparticles on Candida albicans biofilms: an ultrastructural study. J Nanobiotechnology 2015; 13: 91.
Loo CY, Rohanizadeh R, Young PM, et al. Combination of Silver Nanoparticles and Curcumin Nanoparticles for Enhanced Anti -biofilm Activities. J Agric Food Chem 2016; 64(12):2513-22.
Fabrega J, Zhang R, Renshaw JC, et al. Impact of silver nanoparticles on natural marine biofilm bacteria. Chemosphere 2011; 85(6): 961-6.
Argueta-Figueroa L, Morales-Luckie RA, Scougall-Vilchis RJ, et al. Synthesis, characterization and antibacterial activity of copper, nickel and bimetallic Cu-Ni nanoparticles for potential use in dental materials. Prog Nat Sci 2014; 24(4): 321-328.