Journal of Medical Bacteriology 2017. 6(3, 4):31-43.

Evaluation of Antimicrobial Activity of Probiotic Lactobacillus Strains against Growth and Urease Activity of Proteus spp.
Leila Goudarzi, Rouha Kasra Kermanshahi, Zahra Moosavi-Nejad, Mohammad Mehdi Soltan Dalla


Background:    Nowadays, the use of probiotic bacteria for the prevention and treatment of urinary tract infections is growing. Lactobacillus, as probiotic bacterial genus, is well known for its benefits for the human health.

Methods:      The effects of partially purified antimicrobial compounds (bacteriocins and biosurfactants) of Lactobacillus strains was assessed and their capacity to in vitro inhibit growth and urease production of various strains of Proteus spp, was studied. Inhibition of the urease production of Proteus spp. at sub-MIC levels was screened using spectrophotometry method.  

Results:   Results revealed that semi-purified bacteriocins of L. acidophilus and L. plantarum showed a greater inhibitory activity on the bacterial urease, compared to biosurfactants of L. rhamnosus, L. casei and L. fermentum (P < 0.05).

Conclusion:    It can be concluded that bacteriocins may affect Proteus pathogenesis by inhibition of the bacterial urease activity and therefore eliminate the stone formation by these bacteria.


Antimicrobial activity, Lactobacillus spp., Probiotic bacteria, Proteus spp., Ureas.

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Mobley HL, Warren JW. Urease-positive bacteriuria and obstruction of long-term urinary catheters. J Clin Microbiol 1987; 25(11): 2216–17.

Mobley HL, Island MD, Massad G. Virulence determinants of uropathogenic Escherichia coli and Proteus mirabilis. Kidney Int Suppl 1994; 47: S129–36.

Rozalski A, Sidorczyk Z, Koteleko K. Potential virulence factor of Proteus bacilli. Microbial Mol Biol Rev 1997; 61(1): 65–89.

Seneca H, Peer P, Nally R. Microbial urease. Nature (London) 1962; 193, 1106-107.

Todd MJ, Hausinger RP. Competitive inhibitors of Klebsiella aerogenes urease. J Biol Chem 1989; 264(27): 15835–42.

Li X, Zhao, H, Lockatell, CV, Drachenberg, CB, et al. Visualization of Proteus mirabilis within the matrix of urease-induced bladder stones during experimental urinary tract infection. Infect Immun 2002; 70(1): 389–94.

Rosenstein IJM, Hamilton-Miler JMT. Inhibitors of urease as chemotherapeutic agents. Crit Rev Microbiol 1984; 11(1): 1–12.

Reid G, Cook RL, Bruce AW. Examination of strains of Lactobacilli for properties that may influence bacterial interference in the urinary tract. J Urol 1987; 138(2): 330–5.

Psomas E. Some probiotic properties of yeast isolates from infant feces and feta cheese. Int J Food Microbiol 2001; 69(1-2): 125–33.

Klaenhammer TR. Bacteriocins of lactic acid Bacteria. Biochimie 1988; 70(3): 337–49.

Goudarzi L, Kermanshahi RK, Mousavinezhad Z, et al, Antimicrobial and anti-swarming effects of bacteriocins and biosurfactants from probiotic bacterial strains against Proteus spp. J Med Bacteriol. 2016; 5(5, 6): 1–12

Hanlin MB, Kalchayanand N, Ray P, Ray B. Bacteriocins of lactic acid bacteria in combination have greater antibacterial activity. J Food Prot. 1993; 56(3): 252–5.

Schillinger U, Lucke, FK. Antibacterial activity of Lactobacillus sake isolated from meat. Appl Environ Microbiol 1989; 55(8): 1901–6.

Rodrigues LR, Banat IM, Teixeira JA, et al. Biosurfactants: potential applications in medicine. J Antimicrob Chemother 2006; 57(4): 609–18.

Saravanakumari P, Mani K. Structural characterization of a novel xylolipid biosurfactant from Lactococcus lactis and analysis of antibacterial activity against multi-drug resistant pathogens. Bioresource Technology 2010; 101(22): 8851–4.

Busscher H, Neu T, van der Mei HC. Biosurfactant production by thermophilic dairy streptococci. Appl Microbiol Biotechnol 1994; 41(1): 4–7.

Rodrigues L, van der Mei H, Teixeira J, et al. Biosurfactant from Lactococcus lactis 53 inhibits microbial adhesion on silicone rubber. Appl Microbiol Biotechnol 2004; 66(3): 306–11.

Velraeds MC, Mei HC, Reid G, Busscher HJ. Inhibition of initial adhesion of uropathogenic Enterococcus faecalis by biosurfactants from Lactobacillus isolates. Appl Environ Microbiol 1996; 62(6): 1958–63.

Tomas MS, Ocana VS, Wiese B, et al. Growth and lactic acid production by vaginal Lactobacillus acidophilus CRL 1259 and inhibition of uropathogenic Escherichia coli. J Med Microbiol 2003; 52(12): 1117–24.

Fimland G, Johnsen L, Axelsson L, et al. A C-terminal disulfide bridge in pediocin-like bacteriocins renders bacteriocin activity less temperature dependent and is a major determinant of the antimicrobial spectrum. J Bacteriol 2000; 182(9): 2643–8.

Lash BW, Mysliwiec TH, Gourama H. Detection and partial characterization of broad-range bacteriocin produced by Lactobacillus plantarum (ATCC 8014). Food Microbiol 2005; 22(2-3): 199–204.

Han KS, Imm JY, Oh S, Jeon WM, Kim SH. Bacteriocin produced by Lactobacillus acidophilus ATCC 4356: characterization and purification. J Food Sci Biotechnol 2002; 11(5): 531–6.

Ogunbanwo ST, Sanni AI, Onilude AA. Characterization of bacteriocin produced by Lactobacillus plantarum F1 and Lactobacillus brevis OG1. Afr J Biotechnol 2003; 2(8): 219–27.

Nowroozi J, Mirzaii M, Norouzi M. Study of Lactobacillus as probiotic bacteria. Iranian J Publ Health 2004; 33(2): 1–7.

Barefoot SF, Klaenhammer TR. Detection and activity of lactacin B. A bacteriocin produced by Lactobacillus acidophilus. Appl Environ Microbiol 1983; 45(6): 1808–15.

Velraeds MM, Belt-Gritter BV, Mei VD, et al. Interference in initial adhesion of uropathogenic bacteria and yeasts to silicone rubber by a Lactobacillus acidophilus biosurfactant. J Med Microbiol 1998; 47(12): 1081–5.

Gudina EJ, Teixeira JA, Rodrigues LR. Biosurfactant-producing Lactobacilli: screening, production profiles and effect of medium composition. Appl Environ Soil Sci 2011; 201254: 1–9.

Saravanakumari P, Mani K. Structural characterization of a novel xylolipid biosurfactant from Lactococcus lactis and analysis of antibacterial activity against multi-drug resistant pathogens. Bioresource Technology 2010; 101(22): 8851–4.

Mobley HLT, Cortesia MJ, Rosenthal LE, et al. Characterization of urease from Campylobacter pylori. J Clin Microbiol 1988; 26(5): 831–6.

Braude AI, Siemienski J, Siemiensky I. Role of bacterial urease in experimental pyelonephritis. J Bacteriol 1960; 80: 171.

Yasmeen R, Hashmi AS, Anjum AA, et al. Antibacterial activity of indigenous herbal extracts against urease producing bacteria. J Anim Plant Sci 2012; 22(2): 416–9.

Amtul Z, Follmer C, Mahboob S, et al. Germa-γ-lactones as novel inhibitors of bacterial urease activity. Biochem Biophys Res Comm 2007; 356(2):457–63.

Chang PS, Byungse S, Strockbine NA, et al. In vitro inhibitory activities of urea analogues on bacterial urease. Arch Pharm Res 1986; 9: 163–7.

Benini S, Wojciech R, Rypniewski K, et al. Molecular details of urease inhibition by boric acid: insights into the catalytic mechanism. J Am Chem Soc 2004; 126(12): 3714–5.

Wills ED, Wormall A. Studies on suramin 9, the action of the drug on some enzymes. Biochemistry 1950; 47(2): 158–70.

Kuin CM. Effect of organic mercurials and sulfhydryl compounds on the urease activity of Proteus: inhibition by urine and ascorbic acid. Antimicrob Agents Chemother 1976; 10(3): 503–6.

Lim IS, Lee HS, Kim WY. The effect of lactic acid bacteria isolates on the urinary tract pathogens to infants in vitro. J Korean Med Sci 2009; 24(Suppl 1): S57–S62.

Mohankumar A, Murugalatha N. Characterization and antibacterial activity of bacteriocin producing Lactobacillus isolated from raw cattle milk sample. Int J Biol 2011; 3(3): 128–43.

Sahl HG, Brandis H. Efflux of low-Mr substances from the cytoplasm of sensitive cells caused by staphylococcin like agent pep 5. Microbiol Let 1983; 16(1): 75–9.

Prashant S, Tomar K, Singh R, et al. Phenotypic and genotypic characterization of lactobacilli from Churpi cheese. Dairy Sci Technol 2009; 89(6): 531–40.

Hammes WP, Vogel RF. The lactic acid bacteria, the genera of lactic acid bacteria. Glasgow BAaP, editor. Chapman and Hall: 2; 1995.

Fiechter A. Biosurfactants: moving towards industrial application. Trends Biotechnol 1992; 10(6): 208–17.

Jenny K, Deltrieu V, Kappelli O. Lipopeptide production by Bacillus licheniformis in: "Biosurfactant: Production, Properties, Applications",ed Kosaric N; Marcel Dekker. 1993:135–56.

Tahmourespour A, Salehi R, Kermanshahi RK, et al. The anti-biofouling effect of Lactobacillus fermentum-derived biosurfactant against Streptococcus mutans. Biofouling 2011; 27(4): 385–92.

Swift S, Throup JP, Williams P, et al. Quorum sensing a population-density component in the determination of bacterial phenotype. Trends Biochem Sci 1996; 21(6): 214–9.

Ebrel L, Winson MK, Sternberg C. et al. Involvement of N-acyl-l-homoserine lactone autoinducers in controlling the multicellular behavior of Serratia liquefaciens. Mol Microbiol 1996; 20(1): 127–36.


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