Antimicrobial Activities of some Plant Extracts against Phytopathogenic Fungi and Clinical Isolates in Iran

  • Mohammad Reza Majid Khoshkholgh Pahlavia Department of Microbiology, Faculty of Basic Sciences, Lahijan Branch, Islamic Azad University, Lahijan, Iran.
  • Reza Kazemi Darsanaki Young Researchers and Elites Club, Lahijan Branch, Islamic Azad University, Lahijan, Iran.
  • Sirus Bidarigh Department of Agriculture, Lahijan branch, Islamic Azad University, Lahijan, Iran.
Keywords: Antimicrobial activity, Oxalis corniculata, Plant extracts, Peganum harmala, Salvia officinalis

Abstract

Background:       Natural products from plants as environmentally safe options have received attention for controlling various phytopathogenic diseases. In this study, the antimicrobial activities of three plant aqueous and alcoholic extracts (Salvia officinalis, Peganum harmala and Oxalis corniculata) against phytopathogenic fungi (10 fungal isolates associated with diseased tomato fruits) and clinical isolates (10 bacterial isolates) and Candida albicans, as a pathogenic yeast model, were investigated in the Gilan province of northern Iran. Methods:     After phytochemical screening of plant extracts, antimicrobial activity of the extracts evaluated by standard methods for determination of MIC and MBC. Results of the phytochemical screening of aqueous and alcoholic leaf extracts of the selected three plants revealed the presence of saponins, steroids, tannins, glycosides, alkaloids and flavonoids. Results:     All three extracts of the plants tested showed varying degrees of antimicrobial activities against both phytopathogenic fungi and bacteria. Generally, the methanol extracts were more active than other extracts for S. officinalis and P. harmala, whereas for O. corniculata water extract showed more antimicrobial activity. Conclusion:   The study has been able to establish and document the important medicinal plants which can be used in the management of phytopathogens and infectious diseases in Iran.

References

1. Littleton J, Rogers T, Falcone D. Novel approaches to plant drug discovery based on high throughput pharmacological screening and genetic manipulation. Life Sci 2005; 78: 467-75.
2. Duffy CF, Power RF. Antioxidant and antimicrobial properties of some Chinese plant extracts. Int J Antimicrob Agents 2001; 17(6):527-9.
3. Eldeen I, Elgorashi E, Van Staden J. Antibacterial, anti-inflammatory, anti-cholinesterase and mutagenic effects of extracts obtained from some trees used in South African traditional medicine. J Ethnopharmacol 2005; 102(3):457-64.
4. Dellavalle PD, Cabrera A, Alem D, et al. Antifungal activity of medicinal plant extracts against phytopathogenic fungus Alternaria spp. Chil. J. Agr. Res 2011; 71(2):231.
5. Barnett HL, Hunter BB. Illustrated General of Imperfect fungi, t3rd edition. Burgess Publishing Co. Minnesota. 1972, pp.241.
6. Mathur SB. Kongsdal SO. Common laboratory seed health testing methods for detecting fungi. International Seed Testing Association, Copenhagen, Denmark, 2003, pp.357-64.
7. Adejumo TO, Ikotun T, FIorin DA. In-vitro anti-fungal properties of some botanicals on Protomycopsis phaseoli p. 13 in: Book of Abstract, 28 Annual Conference of Nigerian Society for Plant Protection at Cocoa Research Institute of Nigeria (CRIN), Idi-Ayunre Ibadan, 24th-28th 2000; pp.33.
8. Trease GE, Evans WC. Pharmacognosy, 13th edition, Balliere Tindall, London, 1989; pp.176-80.
9. Harborne JB. Phytochemical methods, 2nd edition, Chapman and Hall publications, London, Newyork. 1984; pp.288.
10. Muttalib LY, Naqishbandi AM. Antibacterial and phytochemical study of Iraqi Salvia officinalis leave extracts. Iraqi J Pharm Sci 2012; 21(1):93-7.
11. Miladinović D, Miladinović L. Antimicrobial activity of essential oil of sage from Serbia. FU Phys Chem Technol 2000; 2(2):97-100.
12. Shiri Y, Solouki M, Saeidi S. Activity of some Iranian plant extracts against multi-drug resistant human pathogens isolated from urinary tract infections. Zahedan J Res Med Sci 2014; 16(10):50-4.
13. Moghadam MS, Maleki S, Darabpour E, et al. Antibacterial activity of eight Iranian plant extracts against methicillin and cefixime restistant Staphylococcous aureus strains. Asian Pac J Trop Dis 2010; 3(4):262-5.
14. Lopez A, Hudson J, Towers G. Antiviral and antimicrobial activities of Colombian medicinal plants. J Ethnopharmacol 2001; 77(2):189-96.
15. Rahman MS, Khan MMH, Jamal M. Anti-bacterial evaluation and minimum inhibitory concentration analysis of Oxalis corniculata and Ocimum santum against bacterial pathogens. Biotechnology 2010; 9(4):533-6.
16. Satish S, Raveesha K, Janardhana G. Antibacterial activity of plant extracts on phytopathogenic Xanthomonas campestris pathovars. Lett Appl Microbiol 1999; 28(2):145-7.
17. S Maji S, Dandapat P, Ojha D, et al. In vitro antimicrobial potentialities of different solvent extracts of ethnomedicinal plants against clinically isolated human pathogens. J. phytol 2010; 2(4):57-64.
18. Obi VI, Onuoha C. Extraction and characterization methods of plants and plants products. In: Biological and Agricultural Techniques. Ogbulie JN., Ojaiko OA (eds). Websmedia publishers, Owerri. 2000; pp.271-86.
Published
2018-10-07
How to Cite
1.
Majid Khoshkholgh Pahlavia MR, Kazemi Darsanaki R, Bidarigh S. Antimicrobial Activities of some Plant Extracts against Phytopathogenic Fungi and Clinical Isolates in Iran. jmb. 7(3-4):5-6.
Section
Original Articles