Detection of Intracellular Adhesion (ica) Gene and Biofilm Formation Staphylococcus aureus Isolates from Clinical Blood Cultures
AbstractBackground: In fact the biofilms are composed of bacterial cells living inmulticellular structures such as tissues and organs embedded within a self-produced matrix of extracellular polymeric substance (EPS). Ability to attach and biofilm formation are the most important virulence factors Staphylococcus aureus isolates. The aims of this study were to detect intracellular adhesion (ica) locus and its relation to the biofilm formation phenotype in clinical isolates of S. aureus isolated from bloodcultures.Methods: A total of 31 clinical S. aureus isolates were collected from Loghman Hospital of Tehran, Iran. In vitro biofilm formation ability was determined by microliter tissue culture plates. All clinical isolates were examined for determination the ica locus by using PCR method.Results: Twelve (38.7%) of the isolates were strong biofilm producers. The results showed that 18(80.6%) of the isolates carried icaD gene, whereas the prevalence of icaA, icaB and icaC were 51.6%, 45.1% and 77.4% respectively.Conclusions: S. aureus clinical isolates have different ability to form biofilm. This may be caused by the differences in the expression of biofilm related genes, genetic make-up and physiological conditions.
Ghasemian A, Peerayeh SN, Bakhshi B, et al. Accessory Gene Regulator Specificity Groups Among Staphylococcus aureus Isolated From Hospitalized Children. Arch Pediatr 2014; 2 (2): e16096.
GötzF. Staphylococcus and biofilms.Molecular Microbiol 2002; 43 (6): 1367-78.
Jain A, Agarwal A. Biofilm production, a marker of pathogenic potential of colonizing and commensal staphylococci.J Microbiol Methods 2009; 76 (1): 88-92.
Cramton SE, Gerke C, Schnell NF, et al.The intercellular adhesion (ica) locus is present in Staphylococcus aureus and is required for biofilm formation. Infec Immunity 1999; 67 (10): 5427-33.
CueD, Lei MG, Lee CY. Genetic regulation of the intercellular adhesion locus in staphylococci. Front Cell Infect Microbiol 2012; 2 (38).
Stepanović S, Vuković D, Hola V, et al.Quantification of biofilm in microtiter plates: overview of testing conditions and practical recommendations for assessment of biofilm production by staphylococci. APMIS 2007; 115 (8): 891-9.
Oliveira A, Lourdes R. Comparison of methods for the detection of biofilm production in coagulase-negative staphylococci. BMC Research Notes 2010; 3 (1): 260.
Møretrø T, Hermansen L, Holck AL, et al.Biofilm formation and the presence of the intercellular adhesion locus ica among staphylococci from food and food processing environments. Applied and Environmental Microbiol 2003; 69 (9):5648-55.
Donlan RM. Biofilms and device- associated infections. Emerg Infect Dis 2001; 7 (2): 277.
Performance standards for antimicrobial susceptibility testing: Twenty-first info supplement. CLSI, document M100-S21 (ISBN 1-56238-742-1). Clinical and Laboratory StandInstitute, 940 West Valley Road, Suite 1400, Wayne,Pennsylvania 1987 USA 2011.
Atshan SS, Nor Shamsudin M, Sekawi Z,et al. Prevalence of adhesion and regulation of biofilm-related genes in different clones of Staphylococcus aureus.Bio Med Research International 2012;2012: 1-10
Costerton J, Stewart PS, Greenberg E.Bacterial biofilms: a common cause of persistent infections. Science 1999; 284 (5418): 1318-22.
Davey ME, O'toole GA. Microbial biofilms: from ecology to molecular genetics. Microbiol and Mol Bio Rev.2000; 64 (4): 847-67.
Stewart PS, William Costerton J. Antibiotic resistance of bacteria in biofilms. The Lancet. 2001; 358 (9276): 135-8.
Cramton SE, Gerke C, Götz F. In vitro methods to study staphylococcal biofilm formation. Methods Enzymol 2001; 336:239-55.
Mathur T, Singhal S, Khan S, Upadhyay D, Fatma T, Rattan A. Detection of biofilm formation among the clinical isolates of staphylococci: an evaluation of three different screening methods. Indian J Med Microbiol 2006; 24 (1): 25.
Gad GFM, El-Feky MA, El-Rehewy MS, et al. Detection of icaA, icaD genes and biofilm production by Staphylococcus aureus and Staphylococcus epidermidis isolated from urinary tract catheterized patients. J Infect in Developing Countries 2009; 3 (05): 342-51.
Fowler Jr VG, Fey PD, Reller LB, et al.The intercellular adhesin locus ica is present in clinical isolates of Staphylococcus aureus from bacteremic patients with infected and uninfected prosthetic joints. Medical Microbiol Immun 2001; 189 (3): 127-31.
Yazdani R, Oshaghi M, Havayi A, Pishva E, Salehi R, Sadeghizadeh M, et al. Detection of icaAD gene and biofilm formation in Staphylococcus aureus isolates from wound infections. Iranian J Public Health 2006; 35 (2): 25-8.
Kiem S, Oh WS, Peck KR, et al. Phase variation of biofilm formation in Staphylococcus aureus by IS256 insertion and its impact on the capacity adhering to polyurethane surface. J Korean Med Science 2004; 19 (6): 779-82.
El-Shekh NA, Ayoub AM, El-Hendawy HH, et al. In vitro Activity of some Antimicrobial Agents against Intact and Disrupted Biofilms of Staphylococci in the Indwelling Vascular Catheter Patients. World Applied Sci J 2010; 10 (1): 108-20.
Kiedrowski MR, Horswill AR. New approaches for treating staphylococcal biofilm infections. Annals New York Acad Sci 2011; 1241 (1): 104-21.