Detection of icaA/icaD Genes and Biofilm Formation among Clinical Isolates of Staphylococcus aureus from Shiraz, Iran
,
Abstract
Introduction: Biofilm producing Staphylococcus aureus is known as one of the majorcausative agents of infections, failure of implanted devices and persistent infection
among hospitalized patients. The aim of the present study was to determine the
frequency of biofilm producing S. aureus isolates amongst the clinical specimens.
Methods: This cross-sectional study was conducted during 2012 to 2013 in two
teaching hospitals in Shiraz, southwest of Iran. Totally, 345 S. aureus isolates from
various clinical specimens were included. Biofilm producing isolates were
phenotypically detected using Congo Red Agar (CRA) and genotypically by PCR
assay for the icaA and icaD genes.
Results: Of the 345 S. aureus isolates, 42.3% were methicillin-resistant S. aureus
(MRSA) and subsequently 57.7% were methicillin susceptible isolates. The results of
CRA plates showed that 77 (52.7%) and 74 (37.2%) of MRSA and MSSA were
biofilm producing isolates. The frequency of icaA/D genes among MRSA and MSSA
isolates was 127 (87%) and 167 (83.9%), respectively.
Conclusion: Such a high rate of icaA/D harboring S. aureus among clinical isolates
suggest the risk for establishing persistent infections in the hospital settings.
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| Files | ||
| Issue | Vol 4 No 1-2 (2015) | |
| Section | Original Articles | |
| Keywords | ||
| Biofilm Staphylococcus aureus Congo red agar icaA icaD | ||
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