Original Articles

Differentiation of Penicillin Susceptible and Nonsusceptible Streptococcus pneumoniae


Introduction: Streptococcus pneumoniae cause morbidity and mortality in infants and youngerchildren. Because of high prevalence of penicillin resistance, rapid and reliable diagnostictechniques for penicillin non-susceptible S. pneumoniae (PNSSP) are important for preventionand treatment. We investigated the association of the restriction length polymorphism (RFLP)patterns for pbp2b to distinguish between penicillin susceptible and resistant S. pneumoniaeisolates.Methods: In this study, a total of 70 pneumococcal isolates were collected from different clinicalsources. MIC of these isolates was determined and pbp2b gene was amplified by PCR and theywere digested by HaeІІІ enzyme.Results: Of the 70 isolates, 86% (60) and 14% (10) pneumococcal isolates were found to bePNSSP (penicillin intermediate S. pneumoniae (PISP) and penicillin resistant S. pneumoniae(PRSP)) and penicillin susceptible S. pneumoniae (PSSP). In addition, 10 RFLP patterns (A-J)which were based on the HaeІІІ digestion of pbp2b gene were observed. All PSSP isolatesshowed that they belonged to pattern D, whereas, all PNSSP showed 10 different patterns.Conclusion: In general, the present study suggests that RFLP can be a powerful tool indifferentiation between the penicillin resistant and susceptible strains.

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IssueVol 4 No 1-2 (2015) QRcode
SectionOriginal Articles
HaeІІІ Pneumococcal PCR-RFLP pbp2b

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How to Cite
Ahmadi A, khodaei F, Esghaei M, Irajian G, Talebi M. Differentiation of Penicillin Susceptible and Nonsusceptible Streptococcus pneumoniae. J Med Bacteriol. 2015;4(1-2):15-20.