Encapsulation of Platelet in Kefiran Polymer and Detection of Bioavailability of Immobilized Platelet in Probiotic Kefiran as a New Drug for Surface Bleeding
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Abstract
Background: Kefir contains lactic acid bacteria (Lactobacillus, Lactococcus, Leuconostoc, Acetobacter and Streptococcus) and yeasts (Kluyveromyces, Torula, Candida, and Saccharomyces). Kefiran is the polysaccharide produced by lactic acid bacteria in kefir.
Methods: Kefiran was prepared from milk containing 0.5% fat and 10 grams kefir grains and was separated from kefir by ethanol (0.02 gram) following entrapping the platelets to this polymer. Ligand of the platelet-polysaccharide was studied by FTIR.
Results: FTIR results showed that the bands of C-O and C-O-C connections were formed and the polysaccharides had been attached to the receptors of the platelet glycoproteins (GP Ib, GPIIb / IIIa). Stability and encapsulation of the platelet and kefiran were assessed by Coulter Counter. Encapsulation of the platelets by polysaccharide at the beginning caused to reduce the number of platelets following by releasing of 50% of the platelets after 3 hours.
Conclusion: The platelets were encapsulated in kefiran polymer and detected for bioavailability as new drug for surface bleeding. Also, kefiran has antimicrobial and antifungal properties. On the other hand, the existence of nisin in kefiran could be useful as an antibacterial lantibiotic.
Gallego D, Ferrell N, Sun Y, et al. Multilayer micromolding of degradable polymer tissue engineering scaffold. Mater Sci Eng 2008; 28: 353–358.
de Oliveira Leite AM, Miguel MA, Peixoto RS, et al. Microbiological, technological and therapeutic properties of kefir: a natural probiotic beverage. Braz J Microbiol 2013; 44(2): 341–9.
Lanza RP, Langer RS, Vacanti J. Principles of tissue engineering, 2nd Edition. San Diego: CA, Academic Press 2000.
Enikeev R, Development of a new method for determination of exopolysaccharide quantity in fermented milk products and its application in technology of kefir production. Food Chem 2012; 134: 2437-2441.
Wang Y, Ahmed Z, Feng W, et al. Physicochemical properties of exopolysaccharide produced by Lactobacillus kefiranofaciens ZW3 isolated from Tibet kefir. Int J Biol Macromol 2008; 43: 283-288.
Ghasemlou M, Khodaiyan F, Jahanbin K, et al. Structural investigation and response surface optimization for improvement of kefiran production yield from a low-cost culture medium. Food Chem 2012; 133: 383-389.
Tong Z, Ni L, Ling J. Antibacterial peptide nisin: A potential role in the inhibition of oral pathogenic bacteria. Peptides 2014; 60: 32-40.
Handin, R. I. Inherited platelet disorders. ASH Education Program Book 2005; 2005 (1): 396-402.
Lefkovits J, Plow E. F, Topol E. J. Platelet glycoprotein IIb/IIIa receptors in cardiovascular medicine. N Engl J Med 1995; 332(23): 1553-1559.
Chia J. S, Lin Y.L, Lien, H.T, et al. Platelet aggregation induced by serotype polysaccharides from Streptococcus mutans. Infect Immun 2004; 72(5): 2605-2617.
Fischer T. H, Nichols T. C, Scull C. M, et al. Poly-N- Acetyl glucosamine fibers amplify the effectiveness of recombinant factor VIIA on clot formation in heaphilia B canine blood. J Trauma 2011; 71: 71-75.
Piermaria J. A, de la Canal M. L, Abraham A. G. Gelling properties of kefiran, a food-grade polysaccharide obtained from kefir grain. Food Hydrocoll 2008; 22: 1520-1527.
Piermariaa JA, Pionttia A, Garciaa MA, et al. films based on kefiran, an exopolysaccharide obtained from kefir grain: development and characterization. Food Hydrocoll 2009; 236: 84-90.
Rimada PS, Abraham AG. Comparative study of different methodologies to determine the exopolysaccharide produced by kefir grains in milk and whey. Le Lait 2003; 83:79-87.
Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976; 722: 48-54.
Ghasemlou M, Khodaiyan F, Oromiehie A. Rheological and structural characterisation of film-forming solutions and biodegradable edible film made from kefiran as affected by various plasticizer types. Int J Biol Macromol 2011; 49: 814-821.
Piermaria J, Bosch A, Pinotti A, et al. Kefiran films plasticized with sugars and polyols: water vapor barrier and mechanical properties in relation to their microstructure analyzed by ATR/FT-IR spectroscopy. Food Hydrocoll 2011; 25: 1261-1269.
Jenab A, Roghanian R, Golbang N, et al. Comparison of Three Methods of DNA Extraction in Endocervical Specimens for Chlamydia trachomatis Infection by Spectrophotometry, Agarose Gel, and PCR. Arch Immunol Ther Exp 2010; 58: 227–234.
Rodrigues K. L, Caputo L. R. G, Carvalho J. C. T, et al. Antimicrobial and healing activity of kefir and kefiran extract. Int J Antimicrob 2005; 25: 404-408.
Mirhosseini M, Nahvi I, Emtiazi G, et al. Culture-dependent and culture-independent qualitative analysis of dairy products for bacteriocin production by lactic acid bacteria. World Appl Sci 2008; 5(1), 20-24.
Meusnier I, Singer G.A, Landry J. F, et al. A universal DNA mini-barcode for biodiversity analysis. BMC Genomics 2008; 9(1), 214.
Zeale M.R, Butlin R. K, Barker GLA, et al. Taxon-specific PCR for DNA barcoding arthropod prey in bat feces. Mol Ecol Resour 2011; 11: 236–244.
Infrared Spectroscopy IR Absorptions for Representative Available from: jpkc.huanghuai.edu.cn/include/htmleditor /uploadfile/20130309153033372009. pdf. Accessed August 31, 2014.
Davis R, Mauer LJ. Fourier transform infrared (FT-IR) spectroscopy: a rapid tool for detection and analysis of food borne pathogenic bacteria. Cur. Res. Tech and Edu 2010; 2: 1582–1594.
Rowther E.b, Kardooni H, Warr T. Touch-up gradient amplification method. J Biomol Tech 2012; 23(1): 1-3.
Reiner A. P, Kumar P. N, Schwartz S. M, et al. Genetic variants of platelet glycoprotein receptors and risk of stroke in young women. Stroke 2000; 31(7): 1628-1633.
Medrano, M. Fernando Pérez, P. Graciela Abraham, A. Kefiran antagonizes cytopathic effects of Bacillus cereus extracellular factors. Int J Food Microbiol 2008; 122: 1–7.
Chandrapati S, O'Sullivan, D. J. Nisin independent induction of the nisA promoter in Lactococcus lactis during growth in lactose or galactose1. FEMS Microbiol Lett 1999; 170(1): 191-198.
Pablo M. A, Gaforio J. J, Gallego A. M, et al. Evaluation of immunomodulatory effects of nisin containing diets on mice. FEMS Immunol Med Microbiol 1999; 24(1): 35-42.
Van Niel E. W. J, Hahn-Hägerdal, B. Nutrient requirements of lactococci in defined growth media. Appl Microbiol Biotechnol 1999; 52(5): 617-627.
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| Issue | Vol 4 No 3-4 (2015) | |
| Section | Original Articles | |
| Keywords | ||
| Antimicrobial activity Encapsulation Kefiran Platelet Polysaccharides | ||
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