Contamination of Tap Water with Pseudomonas aeruginosa, Legionella pneumophila, and Escherichia coli in Guilan, Iran
AbstractBackground: River and underground waters are main sources of tap water in Guilan, Iran. Overland wastes move into rivers during periods of heavy or extended rain that is very common in the area.Pseudomonas aeruginosa, Legionella pneumophila, and Escherichia coli are main human pathogens with water source. This study is designed to determine the load of these bacteria in main water suppliesof the area.Methods: Samples were collected directly into sterile containers, concentrated by centrifuge,inoculated in enrichment medium and incubated for 3-4 days. DNA was extracted by using commercialkit. Several rounds of PCR was performed to search P. saeroginosa, integron I, Metallo-β-lactamases gene, L. pneumophila, mip gene, and E. coli.Results: About 92.0% of the samples showed bacterial contamination as revealed by PCR with primers of 16S rRNAgene, 9.5% of the samples had L. pneumophila, and 11,1% had Pseudomonas aeruginosa,but Escherichia coli was not detected. We found the mip gene in 66.6% of the samples with L.pneumophila. Metallo-β-lactamasesgene was found in 11.1% of all samples. We also found Integrin 1 in 28.5% of the samples with P. aeruginosa.Conclusion: This study indicates that in spite of chlorination, total bacterial contamination of pot waters in the area is high and contamination with L. pneumophila and P. aeroginosa is considerable. Itmight be related to the biofilm formation and the growth of water microflora. It seems that free residual chlorine is ineffective. We suggest a more effective decontamination procedure based on modern technology.
Pinfold JV. Fecal contamination of water and fingertiprinses as a method for evaluating the effect of low-cost water supply and sanitation activities on fecal-oral disease transmission. II. A hygiene intervention study in rural north-east Thailand. Epidemiol Infect 1990; 105(2): 377–389.
Karikari AY, Ampofo JA. Chlorine treatment effectiveness and physico-chemical and bacteriological characteristics of treated water supplies in distribution networks of Accra-Tema Metropolis, Ghana. Appl Water Sci 2013; 3: 535-543.
Clasen T, Haller L, Walker D, Bartram J, Cairncross S. Cost-effectiveness of water quality interventions for preventing diarrheal disease in developing countries. J Water Health 2007; 5(4): 599-608.
Durmishi BH, Ismaili M, Shabani A, Abduli Sh. Drinking Water Quality Assessment in Tetova Region. Am J Environ Sci 2012; 8(2): 162-169.
Leclerc H, Schwartzbrod L, Dei-Cas E. Microbial agents associated with waterborne diseases. Crit Rev Microbiol 2002; 28(4): 371-409.
Palleroni NJ. Human- and animal-pathogenic pseudomonads. In The Prokaryotes, 2nd ed.; Balows A, Truper HG, Dworkin M, Harder W, Schleifer KH. Eds.; Springer-Verlag: New York, USA; 1992; pp 3086-3102.
Morais PV, Mesquita C, Andrade J, DaCosta M. Investigation of persistent colonization by Pseudomonas aeruginosa -like strains in a spring water bottling plant. Appl Environ Microbiol 1997; 63: 851-856.
Al-Qadiri HM, Al-Holy MA, Lin M, Alami NI, Cavinato AG, Rasco BA. Rapid detection and identification of Pseudomonas aeruginosa and Escherichia coli as pure and mixed cultures in bottled drinking water using fourier transform infrared spectroscopy and multivariate analysis. J Agric Food Chem 2006; 54(16): 5749-5754.
Villari P, Motti E, Farullo C, Torre I. Comparison of conventional culture and PCR methods for detection of Legionella pneumophila in water. Lett Appl Microbiol 1998; 27(2): 106-110.
Pourcel C, Vidgop Y, Ramisse F, Vergnaud G, Tram C. Characterization of a tandem repeat polymorphism in Legionella pneumophila and its use for genotyping . J Clin Microbiol 2003; 41(5): 1819-1826.
Lindsay DSJ, Abraham WH, RonalJ. Fallon RJ, Detection of mip Gene by PCR for diagnosis of Legionnaires' disease. J Clin Microbiol 1994; 32(12): 3068-3069.
Lavenir R, Jocktane D, Laurent F, Nazaret S, Cournoyer B. Improved reliability of Pseudomonas aeruginosa PCR detection by the use of the species-specific ecfX gene target. J Microbiol Methods 2007; 70(1):20-9.
Yan JJ, Hsueh PR, Ko WC, Luh KT, Tsai SH, Wu HM, Wu JJ. Metallo-b-lactamases in clinical Pseudomonas isolates in Taiwan and identification of VIM-3, a novel variant of the VIM-2 enzyme. Antimicrob Agents Chemother 2001; 45: 2224-8.
Yan JJ, Hsueh PR, Lu JJ, Chang FY, Ko WC, Wu JJ. Characterization of acquired b-lactamases and their genetic support in multidrug-resistant pseudomonas aeruginosa isolates in Taiwan: the prevalence of unusual integrons. Journal of Antimicrobial Chemotherapy 2006; 58: 530-536.
Oswald E, Schmidt H, Morabito S, Karch H, Marches O, Caprioli A. Typing of intimin genes in human and animal enterohemorrhagic and enteropathogenic Escherichia coli: characterization of a new intimin variant. Infect Immun 2000; 68: 64-71.
Kariyama R, Mitsuhata R, Chow JW, Clewell DB, Kumon H. Simple and reliable multiplex PCR assay for surveillance isolates of vancomycine-resistant Enterococci. J Clin Micriobiol 2000; 38(8): 3092-3095.
Lau HY, Ashbolt NJ. The role of biofilms and protozoa in Legionella pathogenesis: implications for drinking water. J Appl Microbiol 2009; 107: 368-378.
Gião MS, Wilks SA, Azevedo NF, Vieira MJ, Keevil CW. Comparison between standard cultures and peptide nucleic acid 16S rRNA hybridization quantification to study the influence of physico-chemical parameters on Legionella pneumophila survival in drinking water biofilms. Biofouling 2009: 25: 335-343.
Lehtola MJ, Torvinen E, Kusnetzov J, Pitkanen T, Maunula L, von Bonsdorff CH, et al. Survival of Mycobacterium avium, Legionella pneumophila, Escherichia coli and caliciviruses in drinking water-associated biofilms grown under high-shear turbulent flow. Appl Environ Microbiol 2007; 73: 2854-2859.
Wingender J, Flemming HC. Contamination potential of drinking water distribution network biofilms. Water Sci Technol. 2004; 49 (11–12): 277-286.
Odonkor ST, Ampofo JK. Escherichia coli as an indicator of bacteriological quality of water: an overview. Microbiol Res 2013; 4:e2: 5-11.
Oliver JD. The viable but nonculturable state in bacteria. J Microbiol. 2005; 43: 93-100.
Feldsine PT, Falbo-Nelson MT, Hustead DL. Colicomplete substrate-supporting disc method for confirmed detection of total coliforms and Escherichia coli in all foods: comparative study. J AOAC Int 1993; 76: 988-1005.
Gevaert K, Van Damme J, Goethals M, Thomas GR, Hoorelbeke B, Demol H, et al. Chromatographic isolation of methionine- containing peptides for gel-free proteome analysis: identification of more than 800 Escherichia coli proteins. Mol Cell Proteomics 2002; 1: 896-903.
Liguori G, Cavallotti I, Arnese A, Amiranda C, Anastasi d, Angelillo IF. Microbiological quality of drinking water from dispensers in Italy. BMC Microbiol 2010, 10:19.
Baumgartner A, Grand M. Bacteriological quality of drinking water from dispenser (coolers) and possible control measures. J Food Prot 2006; 69: 3043-3046.
Zanetti F, De Luca G, Sacchetti R. Control of bacterial contamination in microfiltered water dispensers (MWDs) by disinfection.Int J Food Microbiol 2009; 128: 446-452.
Shamabadi N, Ebrahimi M. Use of bacterial indicators for contamination in drinking water of Qom, Iran. Journal of Applied Sciences 2007; 7(17): 2456-2461.
Yassin MM, Amr SS, Al-Najar HM. Assessment of microbiological water quality and its relation to human health in Gaza Governorate, Gaza Strip. Public Health 2006; 120(12): 1177-87.
AlOtaibi EL. Bacteriological assessment of urban water sources in Khamis Mushait Governorate, southwestern Saudi Arabia. Int J Health Geogr 2009 21; 8:16.
Abu-Zeid HA, Aziz MA, Abolfotouh M, Moneim MA. Bacteriologic pot ability of the drinking water in a diarrhea hyper endemic area in southwestern Saudi Arabia. J Egypt Public Health Assoc 1995; 70(3–4):279-91.
Ahmad M, Bajahlan AS. Quality comparison of tap water vs. bottled water in the industrial city of Yanbu (Saudi Arabia). Environ Monit Assess 2009; 159(1–4):1–14.
Delgado-Viscogliosi P, Simonart T, Parent V, Marchand G, Dobbelaere M, Pierot E, et al. Rapid method for enumeration of viable Legionella pneumophila and other Legionella spp in water. Appl Environ Microbiol 2005; 71(7): 408-4096.
Borella P, Montagna MT, Stampi S, et al. Legionella contamination in hot water of Italian hotels. Appl Environ Microbiol 2005; 71(10): 5805-5813.
Holmberg RE. Pavia AT, Montgomery D, Clark JM, Eggert LD. Nosocomial Legionella pneumophila in the neonate. Pediatrics 1993; 92(3): 450-453.
Meenhorst PL, Reingold AL, Groothuis DG, et al. Water-related nosocomial pneumonia caused by Legionella pneumophila serogroup 1 and 10. J Infect Dis 1985; 152(2):356-364.
Tison DL, Seidler RJ. Legionella incidence and density in potable drinking water supplies. Appl Environ Microbiol 1983; 45(1): 337-339.
Ngwenya N, Ncube EJ, Parsons J. Recent advances in drinking water disinfection: successes and challenges. Rev Environ Contam Toxicol 2013; 222:111-70.
|Issue||Vol 5 No 1-2 (2016)|
|Tap water Pseudomonas aeruginosa Legionella pneumophila Escherichia coli PCR|
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