Detection of OXA-Type Carbapenemase Genes in Acinetobacter baumannii Isolates from Nosocomial Infections in Isfahan Hospitals, Iran
,
Abstract
Background: Acinetobacter baumannii as one of the causes of nosocomial infections has become resistant to almost all antimicrobial agents. The emergence of resistance to carbapenems, one of the last drugs on the shelf, is the major concern about A. baumannii antimicrobial resistance. Resistance to carbapenems is mediated by production of class B and D carbapenemases. The aim of this study was to detect the resistance genes including blaOXA-23, 24, 51, and 58 in A. baumannii isolates from nosocomial infections in Isfahan hospitals.
Methods: A total number of 456 clinical specimens were collected from nosocomial infections and evaluated in order to isolate A. baumannii strains. After identification of the isolates, the antibiotic sensitivity to carbapenems was assessed using disk diffusion method. The resistance genes of bla OXA-23, 24, 51, and 58 were detected by multiplex PCR method.
Results: Fifty A. baumannii isolates were isolated from clinical specimens. Fifty two percent of the isolates showed phenotypic resistance to the carbapenems (imipenem and meropenem).
According to PCR results, 88% of resistant isolates had ≥1 blaOXA gene. The frequency of resistant isolates bearing blaOXA-23, blaOXA-24 and blaOXA-58 were 77%, 38% and 15% respectively.
Conclusion: This study showed the high frequency of carbapenem resistance genes among A. baumannii isolates. Therefore, adopting an appropriate strategy to confine the spreading of these strains and also implementing new treatment regimens are necessary.Opazo A, Domínguez M, Bello H, et al. OXA-type carbapenemases in Acinetobacter baumannii in South America. J Infect Dev Ctries 2011; 6(04): 311-6.
Giannouli M, Cuccurullo S, Crivaro V, et al. Molecular Epidemiology of Multidrug-Resistant Acinetobacter baumannii in a Tertiary Care Hospital in Naples, Italy, Shows the Emergence of a Novel Epidemic Clone. J Clin Microbiol 2010; 48(4): 1223-30.
Rodríguez M, Núñez LE, Braña AF, et al. Mutational analysis of the thienamycin biosynthetic gene cluster from Streptomyces cattleya. Antimicrob Agents Chemo 2011; 55
(4): 1638-49.
Bonomo RA, Szabo D. Mechanisms of multidrug resistance in Acinetobacter species and Pseudomonas aeruginosa. Clin Infect Dis 2006; 43 (Supplement 2): S49-S56.
Peleg AY, Seifert H, Paterson DL. Acinetobacter baumannii: Emergence of a Successful Pathogen. Clin Mcrobiol Rev 2008; 21(3): 538-82.
Farahani R, Moniri R, Dastehgoli Farahani K. Multi-Drug Resistant Acinetobacter-Derived Cephalosporinase and OXAsetC Genes in Clinical Specimens of Acinetobacter spp. Isolated From Teaching Hospital. Jundishapur J Microbiol 2013; 6(2): 181-5.
Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing: Twenty-third Informational Supplement, [... provides updated tables for... M02-A11, M07-A9, and M11-A8]. National Committee for Clinical Laboratory Standards; Wayne, PA 2013.
Feizabadi M, Fathollahzadeh B, Taherikalani M, et al. Antimicrobial susceptibility patterns and distribution of blaOXA genes among Acinetobacter spp. isolated from patients at Tehran hospitals. Jpn J Infect Dis 2008; 61(4): 274-8.
Durante-Mangoni E, Zarrilli R. Global spread of drug-resistant Acinetobacter baumannii: molecular epidemiology and management of antimicrobial resistance. Future microbiol 2011; 6(4): 407-22.
Khosroshahi N, Sharifi M. Carbapenem resistant Acinetobacter strains isolated from patients in intensive care units and equipment of health centers in Qazvin in 2006-2007. Iran J Med Microb 2007; 3(1): 33-8.
Farahani Kheltabadi R, Moniri R, Shajari GR, et al. Antimicrobial Susceptibility patterns and the distribution of resistance genes among Acinetobacter species isolated from patients in shahid Beheshti hospital, Kashan. KAUMS Journal (FEYZ) 2009; 12(4): 61-7.
Hashemizadeh Z, Bazargani A, Emami A, et al. Acinetobacter antibiotic resistance and frequency of ESBL-producing strains in ICU patients of Namazi Hospital (2008-2009). J Qazvin Univ Med Sci 2010; 14: 47-53.
Jafari S NS, Kargar M, Abdollahi A, et al. Phenotypical Evaluation of Multi-Drug Resistant Acinetobacter baumannii. Journal of Fasa University of Medical Sciences 2013; 2(4): 254-8.
Hujer KM, Hujer AM, Hulten EA, et al. Analysis of antibiotic resistance genes in multidrug-resistant Acinetobacter sp. isolates from military and civilian patients treated at the Walter Reed Army Medical Center. Antimicrob Agents Chemo 2006; 50(12): 4114-23.
Valencia R, Arroyo LA, Conde M, et al. Nosocomial outbreak of infection with pan– drug-resistant Acinetobacter baumannii in a tertiary care university hospital. Infect Control Hosp Epidemiol 2009; 30(03): 257-63.
Amudhan SM, Sekar U, Arunagiri K, et al. OXA beta-lactamase-mediated carbapenem resistance in Acinetobacter baumannii. Indian J Med Microbiol 2011; 29(3): 269-74.
Zarrilli R, Giannouli M, Tomasone F, et al. Carbapenem resistance in Acinetobacter baumannii: the molecular epidemic features of an emerging problem in health care facilities. J Infect Dev Ctries 2009; 3(05): 335-41.
Morovat T, Bahram F, Mohammad E, et al. Distribution of different carbapenem resistant clones of Acinetobacter baumannii in Tehran hospitals. The new microbiol 2009; 32(3): 265.
Karunasagar A, Maiti B, Shekar M, et al. Prevalence of OXA‐type carbapenemase genes and genetic heterogeneity in clinical isolates of Acinetobacter spp. from Mangalore, India. Microbiol Immunol 2011; 55(4): 239-46.
Roy S, Basu S, Dasgupta S, et al. Carbapenem resistance in Acinetobacter baumannii isolated from blood of neonates with sepsis. Indian J Med Microbiol 2010; 28(4): 416.
Farahani N, Mirnejad R, Ahmadi Z, et al. Molecular Typing of Acinetobacter baumannii Clinical Strains in Tehran by Pulsed-Field Gel Electrophoresis. Journal of Fasa University of Medical Sciences
; 2(4): 259-65.
Jafari R, Karbasizade V. Frequency and Antimicrobial Susceptibility of Infection. Helicobacter 2013; 18(Suppl.1): 5-11.
| Files | ||
| Issue | Vol 4 No 5-6 (2015) | |
| Section | Original Articles | |
| Keywords | ||
| Acinetobacter baumannii Nosocomial Infection OXA-type Carbapenemase Imipenem Meropenem. | ||
| Rights and permissions | |
|
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
