Distribution of Class I Integron among Isolates of Acinetobacter baumannii Recoverd from Burn Patients
-
Abdolaziz Rastegar-Lari
,
Hajar Mohammadi-Barzelighi
,
Mohammad Arjomandzadegan
,
Rahim Nosrati
,
Parviz Owlia
Abstract
Background: Acinetobacter baumannii, is an important opportunistic pathogens responsible for nosocomial infections. The aim of this experiment was to determine prevalence of Class I Integron in A. baumannii strains isolated from burn patients in Mottahari Hospital and the drug susceptibility pattern.
Methods: There were 69 Acinetobacter isolates, 68 (98.5%) were identified as A. baumannii. Antimicrobial susceptibility of these isolates were determined by a disk diffusion method. PCR assay for detection of blaOXA-51 like gene (for identity confirmation) and intI was performed.
Results: The most effective antibiotic for treating A. baumannii was colistin, followed by tetracyclin and tobramycin. The presence of Integron class I was detected in 14.49% of isolates. ESBL and carbapenemase production were observed in 10% and 24.6% of isolates, respectively.
Conclusion: Due to the high resistance of strains lacking Integron I, the findings are although class I integrons are disseminated among clinical isolates of A. baumannii, at present research, they they do not play important role in dissemination of antibiotic resistance genes in Mottahari Hospital in Tehran, Iran.
Pruitt BA, McManus AT. The changing epidemiology of infection in burn patients. World J Surg 1992;16: 57-67.
Peleg AY, Seifert H, Paterson DL.Acinetobacter baumannii:emergence of a successful pathogen Clin Microbiol Rev 2008; 21: 538-82.
Taherikalani M, Maleki A, Sadegifard N, et al. Dissemination of class 1, 2 and 3 Integrons among different multidrug resistant isolates of Acinetobacter baumannii in Tehran hospitals, Iran. PJM 2011;60: 169-74.
Azimi L, Motevallian A, Ebrahimzadeh A, et al. Nosocomial Infections in burned patients in Motahari hospital,Tehran, Iran. Dermato Research Practice 2011; 10: 1- 4.
Asadollahi KH, Taherikalani M, Maleki A, et al. Diversity of aminoglycoside modifying enzyme genes among multidrug resistant Acinetobacter baumannii genotypes isolated from nosocomial infections in Tehran hospitals and their association with class 1 integrons. Acta Microbiologica et Immunologica Hungarica 2011; 58:359-70.
Asadollahi P, Akbari M, Soroush S, et al. Antimicrobial resistance patterns and their encoding genes among Acinetobacter baumannii strains isolated from burned patients Burns; 2012; 38 (8): 1198-203
Stokes HW, Hall RM. A novel family of potentially mobile DNA elements encoding site-specific gene-integration functions: integrons. Mol Microbiol 1989; 3:1669-83.
Navia MM, Ruiz J, Vila J.Characterization of an integron carrying a new class D beta-lactamase (OXA-37) in Acinetobacter baumannii. MicrobDrug Resist 2002; 8: 261-5.
Nemec A, DolzaniL, Brisse SP, et al. Diversity of aminoglycoside- resistance genes and their association with class 1 integrons among strains of pan-European Acinetobacter baumannii clones. J Med Microbiol 2004; 53: 1233-40.
Fournier PE, Vallenet D, Barbe V, et al. Comparative genomics of multidrug resistance in Acinetobacter baumannii. PLoS Genet 2006; 2: 1-7.
Gallego L, Towner KJ. Carriage of class 1 integrons andantibiotic resistance in clinical isolates of Acinetobacter baumannii from northern Spain. J Med Microbiol 2001; 50: 71-7.
Fluit AC, Schmitz FJ. Class 1 integrons, gene cassettes, mobility, and epidemiology. Eur J Clin Microbiol Infect Dis 1999; 18: 761-70.
Hall RM, Brown HJ, Brookes DE, et al. Integrons found in different locations have identical 59 ends but variable 39 ends. J Bacteriol 1994;176: 6286-94.
Bergogne-Berezin E, Towner KJ.Acinetobacter spp. as nosocomial pathogens: microbiological, clinical, and epidemiological features. Clin Microbiol Rev 1996; 9: 148-65.
Turton JF, Woodford N, Glover J, et al. Identification of Acinetobacter baumannii by detection of the blaOXA-51-like carbapenemase geneintrinsic to this species. J Clin Microbiol 2006; 44: 2974-6.
Clinical and Laboratory Standards Institute (CLSI). Performance Standards for Antimicrobial Susceptibility Testing Twenty-first In formational supplement. M100- S21.2011.
Xiaofei J, Zhe Z. Min L, et al.Detection of Extended- Spectrum β- Lactamases in Clinical Isolates of Pseudomonas aeruginosa. Antimicrob Agents Chemother 2006;50: 2990-5.
Saderi H, Lotfalipour H, Owlia P, et al. Detection of metallo-β- lactamase producing Pseudomonas aeruginosa isolated from burn patients in Tehran, Iran. Labmedicine 2010; 4: 609-12.
Koeleman JGM, Stoof J, Van Der Bijl MW, et al. Identification of epidemic strains of Acinetobacter baumannii by integrase gene PCR. J Clin Microbiol 2001; 39: 8-13.
Taghavi M, Rasouli MR, Boddouhi N, et al. Epidemiology of outpatientburns in Tehran: An analysis of 4813 cases. Burns 2010;36: 109-13.
Rajabian MH, Aghaei S, Fouladi V.Analysis of survival and hospitalization time for 2057 burn patients in Shiraz, south-western Iran. Med Sci Monit 2007; 13: 353-5.
Hosseini RS, Askarian M, Assadian O. Epidemiology of hospitalized female burns patients in a burn centre in Shiraz. East Mediterr Health J 2007; 13: 113-18.
Ansari-Lari M, Askarian M.Epidemiology of burns presenting to an emergency department in Shiraz, South Iran. Burns 2003; 29: 579-81.
Groohi B, Alaghehbandan R, Lari AR. Analysis of 1089 burn patients in province of Kurdistan, Iran. Burns 2002; 28: 69-74.
Wurtz R, Karajovic M, Dacumos E, et al. Nosocomial infections in a burn intensive care unit. Burns 1995; 21:181-4.
Antimicrobial susceptibility of bacterial pathogens isolated from patients with bloodstream infections in the USA, Canada and Latin America. Int J Antimicrob Ag 2000; 13: 257-71.
Jarvis WR, Martone WJ. Predominant pathogens in hospital infections. J Antimicrob Chemother 1992; 29: 19-24.
Vincent JL, Bihari DJ, Suter PM, et al. The prevalence of nosocomial infection in intensive care units in Europe. Results of the European Prevalence of Infection in Intensive Care (EPIC) study. EPIC International Advisory Committee. JAMA 1995; 274: 639-44.
Paul M, Weinberger M, Siegman-Igra Y, et al. Acinetobacter baumannii: emergence and spread in Israeli hospitals1997-2002. J Hosp Infect 2005; 60: 256-60.
Minnangati VR, Cunha BA.Acinetobacter baumannii associated arterial line infection. Am J Infect Control 2000; 28: 376-77.
Hanberger J. and the French and Portuguese ICU Study Group.Antibiotic susceptibility among aerobic gram negative bacilli in ICU in 5 European countries. JAMA 1999; 28:67-70.
Montefour K, Frieden J, Hurst S, et al.Acinetobacter baumannii: an emerging multidrug-resistant pathogen in Critical Care. Crit Care Nurs 2008; 28: 15-25.
Hanberger J, and the French and Portuguese ICU Study Group. Antibiotic susceptibility among aerobic gram negative bacilli in ICU in 5 European countries. JAMA 1999; 28: 67-70.
Jimenez-Mejias ME, Becerril B, Marquez-Rivas FJ, et al. Successful treatment of multidrug-resistant Acinetobacter baumannii meningitis with intravenous colistin sulfomethate sodium. Eur JClin Microbiol Infect Dis 2000; 19: 970-1.
Fernandez-Viladrich P, Corbella X, Corral L, et al. Successful treatment of ventriculitis due to carbapenem- resistant Acinetobacter baumannii with intraventricular colistin sulfomethate sodium. Clin Infect Dis 1999; 28: 916-7.
Li J, Nation RL, Milne RW, et al.Evaluation of colistin as an agent against multi-resistant gram- negative bacteria. Int J Antimicrob Agents 2005; 25: 11-25.
Lewis JR, Lewis SA. Colistin interactions with mammalian urothelium. Am J physiol Cellphysiol. 2004; 286: 913-22.
Johannes GM, Koelman JG, Stoof J,et al. Identification of Epidemic Strains of Acinetobacter baumannii by Integrase Gene PCR. J Clinic Microbiol 2001; 10: 8-13.
Japoni S, Japoni A, Farshad SH, et al.Association between Existence of Integrons and Multi-Drug Resistance in Acinetobacter Isolated from Patients in Southern Iran. PJ Microbiol 2011; 60 (2): 163-8.
Perez F, Hujer AM, Hujer KM, et al.Global challenge of multidrug- resistant Acinetobacter baumannii. Antimicrob Agents Chemother 2007;51: 3471-84.
Bratu S, Landman D, Martin DA, et al. Correlation of antimicrobial resistance with beta-lactamases, the OmpA-like porin, and efflux pumps in clinical isolates of Acinetobacter baumannii endemic to New York City. Antimicrob Agents Chemother 2008; 52: 2999-3005.
Yan ZQ, Shen DX, Cao JR, et al.Susceptibility patterns and molecular epidemiology of multidrug-resistant Acinetobacter baumannii strains from three military hospitals in China. Int J Antimicrob Agents 2010; 35: 269-73.
Martinez-Freijo PAC, Fluit FJ. Schmitz VSC, et al. Class I integrons in Gram- negative isolates from different European hospitals and association with decreased susceptibility to multiple antibiotic compounds. J Antimicrob Chemother 1998; 42: 689-96.
Salimi H, Yakhchali B, Owlia P, et al.Molecular Epidemiology and Drug Susceptibility of Pseudomonas aeruginosa Strains Isolated From Burn Patients. Labmedicine 2010; 41: 6-10.
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| Issue | Vol 2 No 1-2 (2013) | |
| Section | Original Articles | |
| Keywords | ||
| Acinetobacter baumannii Burns Carbapenemase Drug Resistance | ||
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