Incidence and Antibiotic Susceptibility Profile of Staphylococcus aureus Isolates from Wounds of Patients at Specialist Hospital, Sokoto, Nigeria
AbstractBackground: Staphylococcus aureus is an important human pathogen causing varieties of mild to life threatening community and hospital on-set infections. This study was carried out to determine the antibiotic susceptibility profile of Staphylococcus aureus isolated from wounds of patients at a tertiary healthcare facility in Sokoto, Nigeria.Methods: All wound swabs obtained from patients with wound infections during the study period were cultured on mannitol salt agar media. The isolates were identified using standard microbiological methods. Antibiotic susceptibility test was carried out on the identified isolates using the modified Kirby-Bauer disc diffusion method and methicillin resistant Staphylococcus aureus (MRSA) test was carried out using Oxacillin agar screen test as described by Clinical and Laboratory Standard Institute (CLSI, 2016).Results: A total of twenty (20) Staphylococcus aureus were isolated from thirty-eight (38) wound specimens investigated. Out of which, five (25.0%) were found to be MRSA. The isolates were resistant to most of the antibiotics tested and susceptible only to Gentamicin (85%), Norfloxacin (80%) and Amoxiclav (50%).Conclusion: The high incidence of Staphylococcus aureus isolates resistant to the commonly used antibiotics in the hospital calls for urgent need to put in place measures to curtail the spread of MRSA infections in the hospital.
Taddesse Z, Tiruneh M, Gizachew M. Staphylococcus aureus and its antimicrobial susceptibility pattern in patients, Nasal carriage of health personnel, and objects at Dessie referral hospital, Northern Ethiopia. Glob J Med Res. 2014; 14(2): 5–13.
Azeez-Akande O. Global trend of methicillin-resistant Staphylococcus aureus and emerging challenges for control. African J Clin Exp Microbiol. 2010; 11(3): 150–8.
Lowy FD. Antimicrobial resistance: the example of Staphylococcus aureus. J Clin Invest. 2003; 111(9): 1265–73.
Livermore DM. Beta-lactamase-mediated resistance and opportunities for its control. J Antimicrob Chemother. 1998; 41(suppl. D): 25–41.
CLSI. M100-S25: Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Fifth Informational Supplement.
CDC. Antibiotic Resistance Threats in the United States, 2013.
Mottola C, Matias CS, Mendes JJ, et al. Susceptibility Patterns of Staphylococcus aureus biofilms in diabetic foot infections. BMC Microbiol. 2016; 16(119): 1–9.
WHO. Antimicrobial Resistance: Global Report on Surveillance. 2014.
Jo A, Ahn J. Phenotypic and genotypic characterisation of multiple antibiotic-resistant Staphylococcus aureus exposed to sub-inhibitory levels of oxacillin and levofloxacin. BMC Microbiol. 2016; 16(170): 1–10.
Olowo-Okere A, Ibrahim YKE, Sani AS, et al. Prevalence of Surgical Site Infection in a Nigerian University Teaching Hospital. J Pharm Allied Sci. 2017; 14(1): 2430–8.
Alabi OS, Obisesan AO, Ola AA. Prevalence of Methicillin-Resistant Staphylococcus aureus and Extended Spectrum betalactamase Producers Among Bacteria Isolated from Infected Wounds in a Tertiary Hospital in Ibadan City. African J Clin Exp Microbiol. 2016; 17(4): 235–42.
Mohammed A, Adeshina GO, Ibrahim YKE. Incidence and Antibiotic Susceptibility Pattern of Bacterial Nigeria. Trop J Pharm Res. 2013; 12(8): 617–21.
Cheesebrough M. District Laboratory Practice in Tropical Countries. Second edition. New York.: Cambridge University Press; 2007.
Tatah AK, Ngunder PJ, Evelyn MS, et al. Risk factors of wound infections in healthcare facilities in Cameroon: aerobic bacterial pathogens and antibiogram of isolates. The Pan African Med J. 2014; 18:6.
Agbagwa OE, Jirigwa CE. Antibiotics Resistance and Plasmid Profile of Staphylococcus aureus from Wound Swabs in Port Harcourt Nigeria. Curr Res Bacteriol. 2015; 8(3): 70–6.
Onaolapo J, Olayinka B, Adeshina G, et al. Susceptibility Pattern of Staphylococcus aureus Isolates from Orthopaedic Patients in ABUTH, Zaria. J Food Ind Microbiol. 2016; 2(1): 1–6.
Dilnessa T, Bitew A. Prevalence and antimicrobial susceptibility pattern of methicillin resistant Staphylococcus aureus isolated from clinical samples at Yekatit Hospital Medical. BMC Infect Dis. 2016; 16(398): 1–9.
Onwubiko NE, Sadiq NM. Antibiotic sensitivity pattern of Staphylococcus aureus from clinical isolates in a tertiary health institution in kano, Northwestern Nigeria. Pan Afr Med J. 2011; 8(4): 1–7.
Shittu A, Oyedara O, Abegunrin F. et al. Characterization of methicillin-susceptible and resistant staphylococci in the clinical setting: a multicentre study in Nigeria. BMC Infect Dis. 2012; 12(286).
Ike B, Ugwu MC, Ikegbunam MN, et al. Prevalence, Antibiogram and Molecular Characterization of Community-Acquired Methicillin-Resistant Staphylococcus aureus in Awka, Anambra Nigeria. Open Microbiol J. 2016; 10: 211–21.
Onanuga A, Awhowho GO. Antimicrobial resistance of Staphylococcus aureus strains from patients with urinary tract infections in Yenagoa, Nigeria. J Pharm Bioallied Sci. 2012; 4(3): 226–31.
Ekrami A, Montazeri EA, Kaydani GA, et al. Methicillin Resistant Staphylococci: Prevalence and susceptibility patterns in a burn centre in Ahvaz from 2013-2014. Iran J Microbiol. 2015; 7(4): 208–13.
Nalwoga J, Tirwomwe M, Onchweri AN, et al. Drug resistant Staphylococcus aureus in Clinical Samples at Kampala International University-teaching Hospital, Bushenyi District, Uganda. Am J Biomed Res. 2016; 4(4): 94–8.
Malley SMO, Emele FE, Nwaokorie FO, et al. Molecular typing of antibiotic-resistant Staphylococcus aureus in Nigeria. J Infect Public Health. 2015; 8(2): 187–93.
Siegel JD, Rhinehart E, Jackson M, Brennan PJ. Guideline for Isolation Precautions: Preventing Transmission of Infectious Agents in Healthcare Settings. American J of Inf Control. 2007; 35(10):S65-S164.
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