The Determination of the Antimicrobial Susceptibility and Antimicrobial Resistance Gene Patterns in L. monocytogenes
AbstractBackground: Listeriosis, a fatal disease for vulnerable groups, has become common in the last decade due to extensive consumption of dairy and meat products. Listeria monocytogenes is an important foodborne opportunistic human pathogen and causal agent of listeriosis. There is a little information about the antibiotic susceptibility and resistance genes pattern of L. monocytogenes. Methods: The present study aimed to evaluate the antibiotic susceptibility and resistance genes pattern of L. monocytogenes isolates from different clinical and environmental sources. Results: The results showed that 88% of the isolates are resistant to streptomycin and 83% to TMP-SMX. Polymerase chain reaction (PCR) amplification of resistance genes showed that the prevalence of ermA, ermB, strA, tetS, tetA, and ermC genes in L. monocytogenes isolates were 0% (0/55), 10.9% (6/55), 78.81% (43/55), 0% (0/55), 27.27% (15/55), and 0% (0/55), respectively. Conclusion: The resistance of the isolates to the antibiotics represents a potential public health risk and indicates the necessity of the bacteriological controls to reduce the contamination of the food samples.
Srinivasan, V., et al., Prevalence of antimicrobial resistance genes in Listeria monocytogenes isolated from dairy farms. Foodbourne Pathogens & Disease, 2005. 2(3): p. 201-211.
Behrooz, S.K., et al., Study of MazEF, sam, and phd-doc putative toxin–antitoxin systems in Staphylococcus epidermidis. Acta Microbiologica et Immunologica Hungarica, 2018(0): p. 1-11.
Lotfollahi, L., et al., Prevalence and antimicrobial resistance profiles of Listeria monocytogenes in spontaneous abortions in humans. African Journal of Microbiology Research, 2011. 5(14): p. 1990-1993.
Kalani, B.S., et al., Genotypic characterization, invasion index and antimicrobial resistance pattern in Listeria monocytogenes strains isolated from clinical samples. Journal of Acute Disease, 2015. 4(2): p. 141-146.
Kalani, B.S., et al., Putative type II toxin-antitoxin systems in Listeria monocytogenes isolated from clinical, food, and animal samples in Iran. Microbial pathogenesis, 2018.
Graves, L.M., et al., Listeria marthii sp. nov., isolated from the natural environment, Finger Lakes National Forest. International journal of systematic and evolutionary microbiology, 2010. 60(6): p. 1280-1288.
Eslami, G., et al., Identification of Listeria monocytogenes Virulence Factors in Women With Abortion by Polymerase Chain Reaction. Archives of Clinical Infectious Diseases, 2014. 9(3).
Lotfollahi, L., et al., Prevalence, antimicrobial susceptibility and multiplex PCR-serotyping of Listeria monocytogenes isolated from humans, foods and livestock in Iran. Microbial pathogenesis, 2017. 107: p. 425-429.
Farber, J. and P. Peterkin, Listeria monocytogenes, a food-borne pathogen. Microbiological reviews, 1991. 55(3): p. 476-511.
Ohadi, E., et al., IDENTIFICATION OF LISTERIA MONOCYTOGENES VIRULENCE FACTORS IN WOMEN WITH ABORTION BY PCR REFER TO MEDICAL CENTERS IN Iran. Iranian Journal of Public Health, 2014. 43(2): p. 213.
Mead, P.S., et al., Food-related illness and death in the United States. Emerging infectious diseases, 1999. 5(5): p. 607.
Warriner, K. and A. Namvar, What is the hysteria with Listeria? Trends in Food Science & Technology, 2009. 20(6): p. 245-254.
Bahador, A., et al., Phenotypic and Genotypic Characteristics of Listeria monocytogenes Isolated From Dairy and Meat Products. Avicenna Journal of Clinical Microbiology and Infection, 2015. 2(3).
Chen, B.Y., et al., Antibiotic resistance in Listeria species isolated from catfish fillets and processing environment. Letters in applied microbiology, 2010. 50(6): p. 626-632.
Conter, M., et al., Characterization of antimicrobial resistance of foodborne Listeria monocytogenes. International journal of food microbiology, 2009. 128(3): p. 497-500.
Morvan, A., et al., Antimicrobial resistance of Listeria monocytogenes strains isolated from humans in France. Antimicrobial agents and chemotherapy, 2010. 54(6): p. 2728-2731.
Chen, J., et al., Prevalence of Listeria in Chinese food products from 13 provinces between 2000 and 2007 and virulence characterization of Listeria monocytogenes isolates. Foodborne pathogens and disease, 2009. 6(1): p. 7-14.
Granier, S.A., et al., Antimicrobial resistance of Listeria monocytogenes isolates from food and the environment in France over a 10-year period. Applied and environmental microbiology, 2011. 77(8): p. 2788-2790.
Jamali, H., B. Radmehr, and D. Meloni, Prevalence of Listeria monocytogenes in Poultry Marketed in Iran: Characterization and Antimicrobial Resistance of the Isolates. Listeria monocytogenes: Incidence, growth behavior and control, 2015: p. 105-116.
Sakaridis, I., et al., Prevalence and antimicrobial resistance of Listeria monocytogenes isolated in chicken slaughterhouses in Northern Greece. Journal of food protection, 2011. 74(6): p. 1017-1021.
Yan, H., et al., Prevalence and characterization of antimicrobial resistance of foodborne Listeria monocytogenes isolates in Hebei province of Northern China, 2005–2007. International journal of food microbiology, 2010. 144(2): p. 310-316.
McEwen, S.A. and P.J. Fedorka-Cray, Antimicrobial use and resistance in animals. Clinical Infectious Diseases, 2002. 34(Supplement_3): p. S93-S106.
Charpentier, E. and P. Courvalin, Antibiotic Resistance in Listeriaspp. Antimicrobial agents and chemotherapy, 1999. 43(9): p. 2103-2108.
Lungu, B., et al., Listeria monocytogenes: antibiotic resistance in food production. Foodborne pathogens and disease, 2011. 8(5): p. 569-578.
Poyart-Salmeron, C., et al., Transferable plasmid-mediated antibiotic resistance in Listeria monocytogenes. The Lancet, 1990. 335(8703): p. 1422-1426.
Charpentier, E., et al., Incidence of antibiotic resistance in Listeria species. Journal of Infectious Diseases, 1995. 172(1): p. 277-281.
Facinelli, B., et al., Genetic basis of tetracycline resistance in food-borne isolates of Listeria innocua. Applied and environmental microbiology, 1993. 59(2): p. 614-616.
Abuín, C.F., et al., Susceptibilities of Listeria species isolated from food to nine antimicrobial agents. Antimicrobial agents and chemotherapy, 1994. 38(7): p. 1655-1657.
Hadorn, K., et al., Genetic characterization of plasmid-encoded multiple antibiotic resistance in a strain ofListeria monocytogenes causing endocarditis. European Journal of Clinical Microbiology & Infectious Diseases, 1993. 12(12): p. 928-937.
Yu, T. and X. Jiang, Prevalence and characterization of Listeria monocytogenes isolated from retail food in Henan, China. Food Control, 2014. 37: p. 228-231.
Zhang, Y., et al., Characterization of Listeria monocytogenes isolated from retail foods. International journal of food microbiology, 2007. 113(1): p. 47-53.
Fugett, E.B., et al., Pulsed-field gel electrophoresis (PFGE) analysis of temporally matched Listeria monocytogenes isolates from human clinical cases, foods, ruminant farms, and urban and natural environments reveals source-associated as well as widely distributed PFGE types. Journal of clinical microbiology, 2007. 45(3): p. 865-873.
Walsh, D., et al., Antibiotic resistance among Listeria, including Listeria monocytogenes, in retail foods. Journal of Applied Microbiology, 2001. 90(4): p. 517-522.