Identification of the Excretion of Brucella Melitensis Vaccine Strain Rev.1 in Lactating Ewes and the Assessment of Antibody Response in their Lambs
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Abstract
Background: Brucellosis is a zoonotic disease and humans get the infection mostly through consumption of raw milk. Vaccination is the best way to control brucellosis and Iran uses Rev.1 vaccine in sheep and goat flocks. It is evident that the vaccine may shed through milk so it can infect humans. The objective of the present study is to assess the shedding of the vaccine in lactating ewes and its possible immunity in their lambs through milk feeding.
Methods: In a two-month period post-parturition, reduced dose Rev.1 vaccine was injected to 50 parturited ewes. From the first day of vaccination, mixed milk samples were collected and continued for 2 months. Then the samples were tested by PCR method and the sera from 70 lambs of the examined ewes were tested by modified Rose Bengal test while they were feeding milk.
Results: From the 6th day until the 27th day post-vaccination, PCR represented the DNA of Rev.1 in the milk samples. All the lamb’s sera were negative in the serological test.
Conclusion: As the presence of Rev.1 in milk was confirmed in this study, it is important to consider the role of the vaccine strain as a risk of infection in humans. Moreover, as the serological response in the lambs was negative, it seems that the vaccine strain didn’t immunize the lambs through milk feeding so the vaccination of lambs is necessary in small ruminant’s flocks.
2. Alton G. Brucella melitensis. In: Nielsen, Duncan (Eds.), Animal Brucellosis. CRC Press, Florida 1990; pp. 383-409.
3. Esmaeili H, Ekhtiyar Zadeh H, Ebrahim, et al. Evaluation of the national sheep and goat brucellosis control program in Iran. J Arak Uni Med Sci 2012; 14(7):9-20.
4. Corbel MJ. Brucellosis: an overview. Emerg Infect Dis 1997; 3:213-21.
5. Hensel ME, Garcia-Gonzalez DG, Chaki SP, et al. Vaccine candidate Brucella melitensis 16MΔvjbR is safe in a pregnant sheep model and confers protection. mBio 2020; 5(3):1-14.
6. Esmaeili H, Esmaeili H, Amiri k. The effects of brucellosis vaccination in domestic animal on human brucellosis in Iran. Razi J Med Sci 2013; 20(109):80-6.
7. Jiménez de Bagués MP, Marin CM, Barberán M, et al. Responses of ewes to B. melitensis Rev 1 vaccine administrated by subcutaneous or conjunctival routes at different stages of pregnancy. Ann Rech Vet 1989; 20:205-13.
8. Blasco JM. Control and eradication strategies for Brucella melitensis infection in sheep and goats. Prilozi 2010 ; 31:145-65.
9. World Organization for Animal Health, 2018. Manual of diagnostic tests and vaccines for terrestrial animals, 8th ed. Paris.
10. Esmaeili H, Dehghan M. Small ruminant brucellosis vaccination in Iran. Iran J of Immunol 2012; 9:235.
11. Pappas G, Papadimitriou P, Akritidis N, et al. The new global map of human brucellosis Lancet Infect Dis 2006; 6(2):91-9.
12. Phillips RW, Elzer PH, Robertson GT, et al. A Brucella melitensis high-temperature-requirement A (htrA) deletion mutant is attenuated in goats and protects against abortion. Res Vet Sci 1997; 63(2):165-7.
13. Higgins JL, Gonzalez-Juarrero M, Bowen RA. Evaluation of shedding, tissue burdens, and humoral immune response in goats after experimental challenge with the virulent Brucella melitensis strain 16 M and the reduced virulence vaccine strain Rev. 1. PLoS One 2017; 12:e0185823.
14. Saeedzadeh A, Sharifiyazdi H, Firouzi R, et al. Molecular characterization of Brucella melitensis Rev.1 strain in aborted sheep and goats in Iran. Comp Clin Pathol 2013; 22: 409-12.
15. Ponsart C, Riou M, Locatelli Y, et al. Brucella melitensis Rev.1 vaccination generates a higher shedding risk of the vaccine strain in Alpine ibex (Capra ibex) compared to the domestic goat (Capra hircus). Vet Res 2019; 50(100):1-13.
16. Bricker B, Halling, S, Differentiation of Brucella abortus bv. 1, 2, and 4, Brucella melitensis, Brucella ovis, and Brucella suis bv. 1 by PCR. J Clin Microbiol 1994; 32(11):2660-6.
17. Coelho AM, Pinto M, Garcia Diez J, et al. Impact of B.melitensis Rev-1 vaccination on brucellosis prevalence. Turk J Vet Anim Sci 2015; 39:261-70.
18. Elberg SS, Meyer KF. Immunization of goats against brucellosis. (Summary of the experiments concerning the isolation, properties and behavior of vaccine strain). An Fac Med Lima 1959; 42:83-99
19. Alamian S, Bagheri Nejad R, Jalali HR, et al. Innocuousness of conjunctival vaccination with Brucella melitensis strain Rev.1 in pregnant Iranian fat- tailed ewes. Vet Sci Dev 2015; 5(5997):112-14.
20. Pishva E, Salehi M, First report of isolation of Brucella melitensis, vaccine strain Rev.1 as a source of cattle infection in Iran. J Sci I R Iran 2008; 19(1):19-23.
21. El Idrissi AH, Benkirane A, El Maadoudi M, et al. Comparison of the efficacy of Brucella abortus strain RB51 and Brucella melitensis Rev 1. live vaccines against experimental infection with Brucella melitensis in pregnant ewes. Rev Sci Tech Off int Epiz 2001; 20(3):741-7.
22. Squarcione S, Maggi P, Caputo S, et al. A case of human brucellosis caused by accidental injection of animal vaccine. G Ital Med Lav 1990; 12:25-6.
23. Elberg SS, Faunce Jr K. Immunization against Brucella infection. VI. Immunity conferred on goats by a nondependent mutant from a streptomycin-dependent mutant strain of Brucella melitensis. J Bacteriol 1957; 73:211-17.
24. Farahat LF, Abdelwahab MG, Ebied MH, et al. Evaluation of oral immunization of sheep with Brucella melitensis vaccine Rev.1 in combination with flagellin against a virulent Brucella melitensis 16 M strain. BVMJ 2015; 29(2):200-7.
| Files | ||
| Issue | Vol 10 No 1-2 (2021) | |
| Section | Original Articles | |
| Keywords | ||
| Abortion Brucellosis Malta fever Rev.1 vaccine Small ruminant | ||
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