Evaluation of Coxiella burnetii Excretion in Parturition Discharge of Goats with Full Term Delivery Using PCR Method
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Abstract
Background: Coxiella burnetii the causative agent of Q fever and one of the most important abortifacient agents in goats, is a gram negative bacteria. During an outbreak of coxiellosis, the infected animals shed Coxiella burnetii through vaginal discharges, feces, milk and the urine. The present study focused on the presence of Coxiella in the parturition discharges of goats with full-term delivery.
Methods: A total of 25 goats in their first parturition and 25 other goats in their second parturition with no clinical signs of infection caused by Coxiella burnetti, were randomly selected and examined. The vaginal swab samples which had been collected from the animals were extracted and tested by PCR.
Results: In the 25 goats that had parturated for the first time, 19 positive samples were detected (76%). Eighteen samples that had been collected from other goats in their second parturition ,had C.burrenti DNA in their vaginally swabs (72%). The infection rate in goats that had partuated for the first time ,was higher than the goats in their second parturition, had though the difference was not significant.
Conclusion: According to the high prevalence of Coxiella burrentii,the risk of abortion in goats and also as coxiellosis is a zoonotic disease, it is necessary to pay more attention to the bacterium and to conduct appropriate strategies for prevention of disease.
2. Ammerdorffer A, Roest HJ, Dinkla A, et al. The effect of C.burnetii infection on the cytokine response of PBMCs from pregnant goats. PLoS One 2014; 9(10):1-9.
3. Agerholm JS. Coxiella burnetii associated reproductive disorders in domestic animals- a critical review. Acta Vet Scand 2013; 55(1):1-11.
4. Alvarez-Alonso R, Basterretxea M, Barandika JF, et al. A Q fever outbreak with high rate of abortions at a dairy goat farm: Coxiella burnetii shedding, environmental contamination, and viability. Appl Environ Microbiol 2018; 84(20):1-11.
5. Canevari JT, Firestone SM, Vincent G, et al. The prevalence of Coxiella burnetii shedding in dairy goats at the time of parturition in an endemically infected enterprise and associated milk yield losses. BMC Vet Res 2018; 14(1):1-9.
6. Alvarez-Alonso R, Zendoia II, Barandika JF, et al. Monitoring Coxiella burnetii infection in naturally infected dairy sheep flocks throughout four lambing seasons and investigation of viable bacteria. Front Vet Sci 2020; 7(10:352):1-15.
7. Bauer B, Prufer L, Walter M, et al. Comparison of Coxiella burnetii excretion between sheep and goats naturally infected with one cattle-associated genotype. Pathogens 2020; 9(8):1-15.
8. Astobiza I, Barandika JF, Ruiz-Fons F, et al. Coxiella burnetii shedding and environmental contamination at lambing in two highly naturally-infected dairy sheep flocks after vaccination. Res Vet Sci 2011; 91(3):58-63.
9. Berri M, Rousset E, Champion JL, et al. Goats may experience reproductive failures and shed Coxiella burnetii at two successive parturitions after a Q fever infection. Res Vet Sci 2007; 83(1):47–52.
10. Roest HJ, van Gelderen B, Dinkla A, et al. Q fever in pregnant goats: pathogenesis and excretion of Coxiella burnetii. PLoS One 2012; 7(11):1-14.
11. Arricau-Bouvery N, Souriau A, Lechopier P, et al. Experimental Coxiella burnetii infection in pregnant goats: excretion routes. Vet Res 2003; 34(4):423-33.
12. Asadi J, Kafi M, Khalili M. Seroprevalence of Q fever in sheep and goat flocks with a history of abortion in Iran between 2011 and 2012. Vet Ital 2013; 49(2):163-8.
13. Esmaeili S, Mohabbati-Mobarez A, Khalili M, et al. High prevalence and risk factors of Coxiella burnetii in milk of dairy animals with a history of abortion in Iran. Comp Immunol Microbiol Infect Dis 2019; 63:127-30.
14. Nokhodian Z, Feizi A, Ataei B, et al. Epidemiology of Q fever in Iran: A systemic review and meta-analysis for estimating serological and molecular prevalence. J Res Med Sci 2017; 22(121):1-9.
15. Khademi P, Ownagh A, Ataei B, et al. Prevalence of C. burnetii DNA in sheep and goats milk in northwest of Iran. Int J Food Microbiol 2020; 331:1-6.
16. Mostafavi E, Molaeipoor L, Esmaeili S, et al. Seroprevalence of Q fever among high-risk occupations in the Ilam province, the west of Iran. PLoS One 2019; 14(2):1-10.
17. Hoover TA, Vodkin MH, Williams JC. A Coxiella burnetii repeated DNA element resembling a bacterial insertion sequence. J Bacteriol 1992; 174(17):5540-8.
18. Ezatkhah M, Alimolaei M, Khalili M, et al. Seroepidemiological study of Q fever in small ruminants from Southeast Iran. J Infect Public Health 2015; 8(2):170-6.
19. de Cremoux R, Rousset E, Touratier A, et al. Coxiella burnetii vaginal shedding and antibody responses in dairy goat herds in a context of clinical Q fever outbreaks. FEMS Immunol Med Microbiol 2012; 64(1):120-2.
20. de Oliveira J, Rozental T, de Lemos E, et al. Coxiella burnetii in dairy goats with a history of reproductive disorders in Brazil. Acta Trop 2018; 183:19-22.
21. Roest HJ, Dinkla A, Koest AP, et al. Experimental Coxiella burnetii infection in non-pregnant goats and the effect of breeding. Vet Res 2020; 51(1):1-9.
22. Rousset E, Berri M, Durand B, et al. Coxiella burnetii shedding routes and antibody response after outbreaks of Q fever-induced abortion in dairy goat herds. Appl Environ Microbiol 2009; 75(2):428-33.
| Files | ||
| Issue | Vol 10 No 3-4 (2021) | |
| Section | Original Articles | |
| Keywords | ||
| Abortion Coxiella burnetii Goat PCR Vaginal discharge | ||
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