Assessment of the Imipenem Antibiotic Effects on the Expression of Staphylococcus saprophyticus Serine-Aspartate Repeat Protein-Encoding Genes Isolated from Clinical Cases
Abstract
Background: Staphylococcus saprophyticus is one of the most common causes of urinary tract infections (UTIs). Antibiotic resistance against imipenem is increasing worldwide. Our study aimed to investigate the effect of imipenem on expression of the S. saprophyticus serine-aspartate repeat protein-encoding genes by isolating multidrug-resistant strains.
Methods: In this descriptive study, 500 specimens were randomly collected from clinical specimens. Firstly, isolates were identified using standard tests. Then, s. saprophyticus species harboring sdrC, D and E genes were detected using multiplex PCR. Antibiotic susceptibility testing and MIC values of imipenem performed and target genes expression detected by Real time PCR.
Results: Out of 500 samples, 387 Staphylococcus species were isolated, among which 155 strains were S. saprophyticus species. PCR data indicated that 36.77% of S. saprophyticus isolates harbored one of sdr family genes. The MIC and subMIC values obtained for S. saprophyticus species treated with imipenem were 125 μg/ml and 225 µg/ml, respectively. Treatment with imipenem induced significant decrease in the expression of sdrC and sdrD genes as fold changes were -1.241 and -1.322, respectively. There was no statistically significant in sdrE gene expression.
Conclusion: The results of our study showed that resistance to imipenem was significantly higher in strains harboring sdrE gene. Also, treatment with imipenem did not cause any significant change in the expression of sdrE gene, which could be a factor in the application of antibiotic resistance by bacteria to this antibiotic. In addition, the present study raises another alarm about the increased risk of antibiotic resistance
2. Rajivgandhi GN, Maruthupandy M, Li JL, et al. Photocatalytic reduction and anti-bacterial activity of biosynthesized silver nanoparticles against multi drug resistant Staphylococcus saprophyticus BDUMS 5 (MN310601). Mater. Sci. Eng. CC. 2020; 114:111024.
3. Ehlers S, Merrill SA. Staphylococcus saprophyticus. 2018.
4. Deutch C. Limited effectiveness of over‐the‐counter plant preparations used for the treatment of urinary tract infections as inhibitors of the urease activity from Staphylococcus saprophyticus. J Appl Microbiol. 2017; 122(5):1380-8.
5. Martínez‐García S, Rodríguez‐Martínez S, Cancino‐Diaz. proteases of Staphylococcus epidermidis: roles as virulence factors and their participation in biofilm. APMIS. 2018; 126(3):177-85.
6. Argemi X, Hansmann Y, Prola K, et al. Coagulase-negative staphylococci pathogenomics. Int J Mol Sci. 2019; 20(5):1215.
7. Broome-Smith J, Edelman A, Spratt BG. Sequence of penicillin-binding protein 5 of Escherichia coli. The target of penicillin: De Gruyter; 2019; p.403-8.
8. El-Gamal MI, Brahim I, Hisham N, et al. Recent updates of carbapenem antibiotics. Eur J Med Chem. 2017; 131:185-95.
9. Hutchings MI, Truman AW, Wilkinson B. Antibiotics: past, present and future. Curr Opin Microbiol. 2019; 51:72-80.
10. Yelin I, Kishony R. Antibiotic resistance. Cell. 2018; 172(5):1136-1.
11. Martins KB, Faccioli PY, Bonesso MF, et al. Characteristics of resistance and virulence factors in different species of coagulase-negative staphylococci isolated from milk of healthy sheep and animals with subclinical mastitis. J Dairy Sci. 2017; 100(3):2184-95.
12. Hashemzadeh M, Dezfuli A, Nashibi R. Study of biofilm formation, structure and antibiotic resistance in Staphylococcus saprophyticus strains causing urinary tract infection in women in Ahvaz, Iran. New New Microbes New Infect. 2021; 39:100831.
13. Lo DS, Shieh HH, Ragazzi SLB, et al. Infecção urinária comunitária: etiologia segundo idade e sexo. Braz. J. Nephrol. 2013; 35(2):93-8.
14. Nwokocha A, Ujunwa F, Onukwuli V, et al. pattern of bacteriuria among asymptomatic secondary school adolescents within Enugu South East Nigeria. Ann Mil Health Sci Res. 2014; 4(5):728-32.
15. Pizauro LJL, Almeida CC, Silva SR, et al. Genome Comparison and Phylogenetic Analysis of Mastitis-Related Staphylococci with a Focus on Adhesion, Bio lm, and Regulatory-Related Genes. 2021.
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| Issue | Vol 10 No 3-4 (2021) | |
| Section | Original Articles | |
| Keywords | ||
| antibiotic resistance imipenem Staphylococcus saprophyticus UTI | ||
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