The Frequency of Bacterial Pathogens of Mastitis and their Antibiotic Susceptibility in Saanen and Alpine Goats
,
Abstract
Background: The purpose of Saanen and Alpine raising farms is milk production, so control of mastitis is essential. Antibiotic resistance among mastitis pathogens has been increased by long-time usage of antibiotics. The aim of the present study was to isolate bacteria from mastitis cases in Saanen and Alpine goats and to determine their antibiotic resistance.
Methods: Milk sample of 26 Saanen and 29 Alpine was cultured on microbiological media. Polymerase chain reaction was performed for the detection of Mycoplasma. Antibiotic resistance of the bacteria was tested by 12 antibacterial disks. Significant difference in resistance levels between Saanen and Alpine was assessed.
Results: Mycoplasma spp. was detected in 12.73% of samples. The isolated bacteria were Escherichia coli (29.1%), Trueperella pyogenes (25.5%), Staphylococcus aureus (16.4%), Streptococcus agalactiae (9.1%), coagulase negative staphylococci (5.5%), and Corynebacterium pseudotuberculosis (1.8%). The significant difference was observed in antibiotic resistance to amoxicillin which was higher in Alpine.
Conclusion: Accordingly using antibiotics more accurate and using antibiogram by clinician are necessary.
1. Smith MC, Sherman, DM., 2009. Goat medicine, 2nd ed. Willly-Blackwell, Chichester.
2. Gökdai A, Sakarya E, Contiero B, et al. Milking characteristics, hygiene and management practices in saanen goat farms: a case of Canakkale province, Turkey. Ital J Anim Sci 2020; 19(1):213-21.
3. Arnal M, Larroque H, Leclerc H, et al. Genetic parameters for first lactation dairy traits in the Alpine and Saanen goat breeds using a random regression test-day model. BMC 2019; 51 (43): 1-15.
4. Keane OM. Symposium review: Intramammary infections-Major pathogens and strain-associated complexity. Int J Dairy Sci 2019; 102 (5):4713–26.
5. Croft A, Duffield T, Menzies P, et al. The effect of tilmicosin administered to ewes prior to lambing on incidence of clinical mastitis and subsequent lamb performance. Can Vet J 2000; 41:306–311.
6. Plummer PJ, Plummer C, 2011. Diseases of the mammary gland. In: Pugh, D.G.(ed), Sheep and goat medicine. Elsevier, pp. 442-450.
7. Cebra CK, Garry FB, Dinsmore RP. Naturally occurring acute coliform mastitis in Holstein cattle. J Vet Intern Med 1996; 10(4): 252–7.
8. Wenz JR, Barrington GM, Garry FB, et al. Bacteremia associated with naturally occurring acute coliform mastitis in dairy cows. J Am Vet Med Assoc 2001; 219(7):976–81.
9. Memon J, Kashif J, Yaqoob M, et al. Molecular characterization and antimicrobial sensitivity of pathogens from sub-clinical and clinical mastitis in Eastern China. Pak Vet J 2013; 33(2):170-4.
10. Abdi RD, Gillespie BE, Vaughn J, et al. Antimicrobial resistance of Staphylococcus aureus isolates from dairy cows and genetic diversity of resistant isolates. Foodborne Pathog Dis 2018; 15(7):449–58.
11. Massé J, Lardé H, Fairbrother JM, et al. Prevalence of antimicrobial resistance and characteristics of Escherichia coli isolates from fecal and manure pit samples on dairy farms in the province of Québec, Canada. Front vet Sci 2021; 8:1-14.
12. Botes A, Peyrot BM, Olivier AJ, et al. Bellstedt, Identification of three novel Mycoplasma species from ostriches in south Africa. Vet Microbiol 2005; 111(3-4):159-69.
13. Wayne, P., 2018. Performance standards for antimicrobial susceptibility testing, 28th ed. Clinical and Laboratory Standards Institute, Pennsylvania.
14. Bradley AJ, Green MJ. Aetiology of clinical mastitis in six Somerset dairy herds. Vet Rec 2001; 148(22): 683-6.
15. Esmaeili, H., Hamedi, M., 2019. Color atlas of sheep and goat diseases of Iran, 1st ed. University of Tehran Press, Tehran.
16. Zhao Y, Liu H, Zhao X, et al. Prevalence and pathogens of subclinical mastitis in dairy goats in China. Trop Anim Health Prod 2014; 47(2): 429-35.
17. Goncagul G, Gunaydin E, Cokal Y. Antimicrobial susceptibility of bacteria isolated from goats with subclinical mastitis in the Southern Marmara region of Turkey. Med Weter 2021; 77(5):258-63.
18. Sarker H, Samad M A. Udder-halve-wise comparative prevalence of clinical and subclinical mastitis in lactating goats with their bacterial pathogens and antibiotics sensitivity patterns in Bangladesh, Bangl. J Med 2011; 9: 137-43.
19. Balemi A, Gumi B, Amenu K, et al. Prevalence of mastitis and antibiotic resistance of bacterial isolates from CMT positive milk samples obtained from dairy cows, camels and goats in two pastoral districts in southern Ethiopia. Animals 2021; 11(6):1-17.
20. McDougall S, Pankey W, Delaney C, et al. Prevalence and incidence of subclinical mastitis in goats and dairy ewes in Vermont, USA. Small Rumin Res 2002; 46(2-3): 65115–121.
21. Constable, P., Hinchcliff, K.W., Done, S., Gruenberg, W., 2017. Veterinary medicine, 11th ed. ELSEVIER, Amesterdam.
22. Jabbar A, Saleem MH, Iqbal MZ, et al. Epidemiology and antibiogram of common mastitis-causing bacteria in beetal goats. Vet World 2020; 13(12): 2596-607.
23. Ali Z, Muhammad G, Ahmad T, et al. Prevalence of caprine sub-clinical mastitis, its etio-logical agents and their sensitivity to antibiotics in indige¬nous breeds of Kohat, Pakistan. Pak J Life Soc Sci 2010; 8(1): 63-7.
24. Begum M, Hossain M, Ershaduzzaman M, et al. Epidemiological studies on subclinical mastitis in dairy goats in Northern Regions of Bangladesh. Bangladesh j Livest Res 2016; 19(1-2): 112-22.
25. Ramprabhu R. Subclinical mastitis in crossbred goats-diagnostic and therapeutic profile. Indian J Vet Med 2019; 39(2):38-39.
26. Rainard P, Foucras G, Fitzgerald JR, et al. Knowledge gaps and research priorities in Staphylococcus aureus mastitis control. Transbound Emerg Dis 2018; 65:149-65.
2. Gökdai A, Sakarya E, Contiero B, et al. Milking characteristics, hygiene and management practices in saanen goat farms: a case of Canakkale province, Turkey. Ital J Anim Sci 2020; 19(1):213-21.
3. Arnal M, Larroque H, Leclerc H, et al. Genetic parameters for first lactation dairy traits in the Alpine and Saanen goat breeds using a random regression test-day model. BMC 2019; 51 (43): 1-15.
4. Keane OM. Symposium review: Intramammary infections-Major pathogens and strain-associated complexity. Int J Dairy Sci 2019; 102 (5):4713–26.
5. Croft A, Duffield T, Menzies P, et al. The effect of tilmicosin administered to ewes prior to lambing on incidence of clinical mastitis and subsequent lamb performance. Can Vet J 2000; 41:306–311.
6. Plummer PJ, Plummer C, 2011. Diseases of the mammary gland. In: Pugh, D.G.(ed), Sheep and goat medicine. Elsevier, pp. 442-450.
7. Cebra CK, Garry FB, Dinsmore RP. Naturally occurring acute coliform mastitis in Holstein cattle. J Vet Intern Med 1996; 10(4): 252–7.
8. Wenz JR, Barrington GM, Garry FB, et al. Bacteremia associated with naturally occurring acute coliform mastitis in dairy cows. J Am Vet Med Assoc 2001; 219(7):976–81.
9. Memon J, Kashif J, Yaqoob M, et al. Molecular characterization and antimicrobial sensitivity of pathogens from sub-clinical and clinical mastitis in Eastern China. Pak Vet J 2013; 33(2):170-4.
10. Abdi RD, Gillespie BE, Vaughn J, et al. Antimicrobial resistance of Staphylococcus aureus isolates from dairy cows and genetic diversity of resistant isolates. Foodborne Pathog Dis 2018; 15(7):449–58.
11. Massé J, Lardé H, Fairbrother JM, et al. Prevalence of antimicrobial resistance and characteristics of Escherichia coli isolates from fecal and manure pit samples on dairy farms in the province of Québec, Canada. Front vet Sci 2021; 8:1-14.
12. Botes A, Peyrot BM, Olivier AJ, et al. Bellstedt, Identification of three novel Mycoplasma species from ostriches in south Africa. Vet Microbiol 2005; 111(3-4):159-69.
13. Wayne, P., 2018. Performance standards for antimicrobial susceptibility testing, 28th ed. Clinical and Laboratory Standards Institute, Pennsylvania.
14. Bradley AJ, Green MJ. Aetiology of clinical mastitis in six Somerset dairy herds. Vet Rec 2001; 148(22): 683-6.
15. Esmaeili, H., Hamedi, M., 2019. Color atlas of sheep and goat diseases of Iran, 1st ed. University of Tehran Press, Tehran.
16. Zhao Y, Liu H, Zhao X, et al. Prevalence and pathogens of subclinical mastitis in dairy goats in China. Trop Anim Health Prod 2014; 47(2): 429-35.
17. Goncagul G, Gunaydin E, Cokal Y. Antimicrobial susceptibility of bacteria isolated from goats with subclinical mastitis in the Southern Marmara region of Turkey. Med Weter 2021; 77(5):258-63.
18. Sarker H, Samad M A. Udder-halve-wise comparative prevalence of clinical and subclinical mastitis in lactating goats with their bacterial pathogens and antibiotics sensitivity patterns in Bangladesh, Bangl. J Med 2011; 9: 137-43.
19. Balemi A, Gumi B, Amenu K, et al. Prevalence of mastitis and antibiotic resistance of bacterial isolates from CMT positive milk samples obtained from dairy cows, camels and goats in two pastoral districts in southern Ethiopia. Animals 2021; 11(6):1-17.
20. McDougall S, Pankey W, Delaney C, et al. Prevalence and incidence of subclinical mastitis in goats and dairy ewes in Vermont, USA. Small Rumin Res 2002; 46(2-3): 65115–121.
21. Constable, P., Hinchcliff, K.W., Done, S., Gruenberg, W., 2017. Veterinary medicine, 11th ed. ELSEVIER, Amesterdam.
22. Jabbar A, Saleem MH, Iqbal MZ, et al. Epidemiology and antibiogram of common mastitis-causing bacteria in beetal goats. Vet World 2020; 13(12): 2596-607.
23. Ali Z, Muhammad G, Ahmad T, et al. Prevalence of caprine sub-clinical mastitis, its etio-logical agents and their sensitivity to antibiotics in indige¬nous breeds of Kohat, Pakistan. Pak J Life Soc Sci 2010; 8(1): 63-7.
24. Begum M, Hossain M, Ershaduzzaman M, et al. Epidemiological studies on subclinical mastitis in dairy goats in Northern Regions of Bangladesh. Bangladesh j Livest Res 2016; 19(1-2): 112-22.
25. Ramprabhu R. Subclinical mastitis in crossbred goats-diagnostic and therapeutic profile. Indian J Vet Med 2019; 39(2):38-39.
26. Rainard P, Foucras G, Fitzgerald JR, et al. Knowledge gaps and research priorities in Staphylococcus aureus mastitis control. Transbound Emerg Dis 2018; 65:149-65.
| Files | ||
| Issue | Vol 11 No 1-2 (2023) | |
| Section | Original Articles | |
| DOI | https://doi.org/10.18502/jmb.v11i1-2.14371 | |
| Keywords | ||
| Alpine Antimicrobial resistance Goat Mastitis Saanen. | ||
| Rights and permissions | |
|
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
How to Cite
1.
Esmaeili H, Shakeri AP, Tajik P, Hamedi M. The Frequency of Bacterial Pathogens of Mastitis and their Antibiotic Susceptibility in Saanen and Alpine Goats. J Med Bacteriol. 2023;11(1-2):19-26.
