Biosynthesis of Zinc Nanoparticles of Capparis Spinosa Plant Extract and the it’s Investigation on Morhpophysiological Properties of the Moringa Olifera Plant
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Abstract
Background: The article aim was biosynthesis of zinc nanoparticles by the fruit and stem of Capparis Spinosa plant extract and the investigation of growth factors and some physiological properties of the M. Oleifera plant.
Methods: Four levels of zinc nanoparticles (0, 125, 250, and 500) ppm were considered as treatments. Water extract of the Capparis Spinosa plant were obtained using 10 grams of stem and fruit were separately dried. Some properties like below information were measured: Ultraviolet-visible spectroscopic analysis, Infrared spectroscopy, Measurement of chlorophyll a and b and carotenoids, total phenol, antioxidant activity, total protein and antioxidant enzymes. The data of this research were factorially conducted in the form of completely randomized blocks with three replications.
Results: The effect of zinc nanoparticles on growth parameters shows that the length and weight of the shoot and root are significant at the five percent probability level. Also, the length and dry weight of shoots and roots are significant at the five percent probability level. The effect of zinc nanoparticles on the concentration of chlorophyll a, chlorophyll b and carbohydrates is significant at the five percent probability level. The main effect of zinc nanoparticles on the amount of total phenol, flavonoid, and DPPH of the M. Oleifera medicinal plant was significant. The effect of stem and fruit extract of zinc nanoparticles on the amount of ascorbate peroxidase, catalase, and guaiacol oxidase enzymes.
Conclusion: The results showed that nano made from fruit and stem significantly increases root length, protein content and total phenol content, and activity of catalase and ascorbate peroxidase enzymes.
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2. Kumar S, Verma PK, Shukla A, et al. Moringa oleifera L. leaf extract induces cell cycle arrest and mitochondrial apoptosis in Dalton's Lymphoma: An in vitro and in vivo study. J Ethnopharmacol. 2023; 302(Pt A):115849.
3. Sharma K, Kumar M, Waghmare R, et al. Moringa (Moringa oleifera Lam.) polysaccharides: extraction, characterization, bioactivities, and industrial application. Int J Biol Macromol 2022; 209(Pt A):763-78.
4. Aoki H, Nakatsuka-Mori T, Ueno Y, et al. Analysis of functional ingredients of tempe-like fermented Moringa oleifera seeds (Moringa tempe) prepared with Rhizopus species. J Biosci Bioeng 2023; 135(4):306-12.
5. Alavi M, Hamblin MR, Mozafari MR, et al. Surface modification of SiO2 nanoparticles for bacterial decontaminations of blood products. Cell Mol Biomed Rep 2022; 2(2):87-98.
6. Alavi M, Rai M, Martinez F, et al. The efficiency of metal, metal oxide, and metalloid nanoparticles against cancer cells and bacterial pathogens: different mechanisms of action. Cell Mol Biomed Rep 2022; 2(1):10-21.
7. Alavi M, Hamblin MR. Antibacterial silver nanoparticles: effects on bacterial nucleic acids. Cell Mol Biomed Rep 2023; 3(1):35-41.
8. Payal, Pandey P. Role of nanotechnology in electronics: a review of recent developments and patents. Recent patents on nanotechnology 2022; 16(1):45-66.
9. Li X, Shi C, Wang S, et al. Uncovering the effect of Moringa oleifera Lam. leaf addition to Fuzhuan Brick Tea on sensory properties, volatile profiles and anti-obesity activity. Food Funct 2023; 14(5):2404-15.
10. Nawaz QU, Kausar R, Jabeen N, et al. Influence of bio fabricated manganese oxide nanoparticles for effective callogenesis of Moringa oleifera Lam. Plant Physiol Biochem 2023; 198:107671.
11. Rasouli H, Popović-Djordjević J, Sayyed RZ, et al. Nanoparticles: A New Threat to Crop Plants and Soil Rhizobia? In: Hayat S, Pichtel J, Faizan M, et al., editors. Sustainable Agriculture Reviews 41: Nanotechnology for Plant Growth and Development. Cham: Springer International Publishing; 2020. p. 201-14.
12. Hasanuzzaman M, Raihan MRH, Nowroz F, et al. Insight into the physiological and biochemical mechanisms of biostimulating effect of Ascophyllum nodosum and Moringa oleifera extracts to minimize cadmium-induced oxidative stress in rice. Environmental science and pollution research international. 2023; 30(19):55298313.
13. Liu R, Liu J, Huang Q, et al. Moringa oleifera: a systematic review of its botany, traditional uses, phytochemistry, pharmacology and toxicity. The Journal of pharmacy and pharmacology 2022; 74(3):296-320.
14. Gali Aba Lulesa T, Beyene D, Ebba M, et al. Water treatment using natural coagulant and electrocoagulation process: A comparison study. Int J Anal Chem 2022; 2022:4640927.
15. Araujo NS, Souza NF, de Lima-Faria JM, et al. Treatment of cosmetic industry wastewater by flotation with Moringa oleifera Lam. and aluminum sulfate and toxicity assessment of the treated wastewater. Environmental science and pollution research international 2022; 29(1):1199-1209.
16. Marschner H. Zinc Uptake from soils. in: Robson AD, editor. zinc in soils and plants: proceedings of the international symposium on ‘zinc in soils and plants’ held at the University of Western Australia, 27–28 September, 1993. Dordrecht: Springer Netherlands; 1993. p. 59-77.
17. Abdoun K, Alsagan A, Altahir O, et al. Cultivation and uses of Moringa oleifera as non-conventional feed stuff in livestock production: a review. Life (Basel, Switzerland) 2022; 13(1):63.
18. Barba-Brioso C, Hidalgo PJ, Fernandez-Landero S, et al. Phytoaccumulation of trace elements (As, Cd, Co, Cu, Pb, Zn) by Nicotiana glauca and Euphorbia segetalis growing in a Technosol developed on legacy mine wastes (Domingo Rubio wetland, SW Spain). Environ Geochem Health. 2023.
19. Mawouma S, Ponka R, Mbofung CM. Acceptability and solubility of iron and zinc contents of modified Moringa oleifera sauces consumed in the Far-north region of Cameroon. Food Sci Nutr 2017; 5(2):344-8.
20. Yusefi-Tanha E, Fallah S, Rostamnejadi A, et al. Responses of soybean (Glycine max [L.] Merr.) to zinc oxide nanoparticles: Understanding changes in root system architecture, zinc tissue partitioning and soil characteristics. The Science of the total environment. 2022; 835:155348.
21. Lindsay W. Inorganic phase equilibria micronutrients in soils. Micronutrients in agriculture 1972:41-57.
22. Chen X, Zhang R, Xing Y, et al. The efficacy of different seed priming agents for promoting sorghum germination under salt stress. PLoS One. 2021; 16(1):e0245505.
23. Dehkourdi EH, Mosavi M. Effect of anatase nanoparticles (TiO2) on parsley seed germination (Petroselinum crispum) in vitro. Biological trace element research 2013; 155(2):283-6.
24. Wu F, Fang Q, Yan S, et al. Effects of zinc oxide nanoparticles on arsenic stress in rice (Oryza sativa L.): germination, early growth, and arsenic uptake. Environmental science and pollution research international 2020; 27(21):26974-81.
25. Baba SA, Malik SA. Determination of total phenolic and flavonoid content, antimicrobial and antioxidant activity of a root extract of Arisaema jacquemontii Blume. Journal of Taibah University for Science 2015; 9(4):449-54.
26. Hara K, Someya T, Sano K, et al. Antioxidant activities of traditional plants in Sri Lanka by DPPH free radical-scavenging assay. Data in brief 2018; 17:870-5.
27. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal biochem 1976; 72(1-2):248-254.
28. Chance B, Maehly A. [136] Assay of catalases and peroxidases. Methods in enzymology 1955; 2:764-75.
29. Mehlhorn H. Ethylene‐promoted ascorbate peroxidase activity protects plants against hydrogen peroxide, ozone and paraquat. Plant, Cell & Environment 1990; 13(9):971-6.
30. Jahantigh Haghighi z, fahmideh l, Fazeli Nasab b. Evaluation and comparison of leaf antioxidant properties and morphological traits of tomato varieties (Lycopersicon esculentum L). Journal of Iranian Plant Ecophysiological Research 2018; 13(50):63-76.
31. Anjum S, Vyas A, Sofi T. Fungi-mediated synthesis of nanoparticles: characterization process and agricultural applications. J Sci Food Agric 2023; 103(10):4727-41.
32. Patil S, Sastry M, Bharde A. Size and shape directed novel green synthesis of plasmonic nanoparticles using bacterial metabolites and their anticancer effects. Front Microbiol 2022; 13:866849.
33. Abdallah Y, Hussien M, Omar MOA, et al. Mung Bean (Vigna radiata) treated with magnesium nanoparticles and its impact on soilborne Fusarium solani and Fusarium oxysporum in clay soil. Plants (Basel) 2022; 11(11):1514.
34. Azeem M, Pirjan K, Qasim M, et al. Salinity stress improves antioxidant potential by modulating physio-biochemical responses in Moringa oleifera Lam. Sci Rep. 2023; 13(1):2895.
35. Rafiq H, Aftab ZE, Anjum T, et al. Bio-fabrication of zinc oxide nanoparticles to rescue mung bean against Cercospora leaf spot disease. Front Plant Sci 2022; 13:1052984.
36. Atteya AKG, El-Serafy RS, El-Zabalawy KM, et al. Exogenously supplemented proline and phenylalanine improve growth, productivity, and oil composition of salted moringa by up-regulating osmoprotectants and stimulating antioxidant machinery. Plants (Basel) 2022; 11(12):1553.
37. Jenish A, Ranjani S, Hemalatha S. Moringa oleifera nanoparticles demonstrate antifungal activity against plant pathogenic fungi. Appl Biochem Biotechnol 2022; 194(10):4959-70.
38. El-Khadragy M, Alolayan EM, Metwally DM, et al. Clinical efficacy associated with enhanced antioxidant enzyme activities of silver nanoparticles biosynthesized using Moringa oleifera leaf extract, against cutaneous leishmaniasis in a murine model of Leishmania major. Int J Environ Res Public Health 2018; 15(5):1037.
39. Shah G, Tu J, Fayyaz M, et al. Moringa oleifera smoke induced positive changes in biochemical, metabolic, and antioxidant profile of rice seedling under cadmium stress. Int J Phytoremediation 2022:1-11.
40. Wyszkowski M, Wyszkowska J, Kordala N, et al. Molecular sieve, halloysite, sepiolite and expanded clay as a tool in reducing the content of trace elements in Helianthus annuus L. on copper-contaminated soil. Materials (Basel, Switzerland) 2023; 16(5):1827.
41. Milla PG, Penalver R, Nieto G. Health Benefits of Uses and Applications of Moringa oleifera in Bakery Products. Plants (Basel). 2021; 10(2):318.
42. Watanabe S, Okoshi H, Yamabe S, et al. Moringa oleifera Lam. in diabetes mellitus: a systematic review and meta-analysis. Molecules. 2021; 26(12):3513.
43. Soumaoro I, Pitala W, Gnandi K, et al. Health Risk assessment of heavy metal accumulation in broiler chickens and heavy metal removal in drinking water using moringa Oleifera seeds in lome, Togo. Journal of health & pollution. 2021; 11(31):210911.
44. Abdelhameed RE, Abdel Latef AAH, Shehata RS. Physiological responses of salinized fenugreek (Trigonellafoenum-graecum L.) plants to foliar application of salicylic acid. Plants (Basel) 2021; 10(4):10.3390
45. Sehsah MD, El-Kot GA, El-Nogoumy BA, et al. Efficacy of Bacillus subtilis, Moringa oleifera seeds extract and potassium bicarbonate on Cercospora leaf spot on sugar beet. Saudi J Biol Sci. 2022; 29(4):2219-29.
2. Kumar S, Verma PK, Shukla A, et al. Moringa oleifera L. leaf extract induces cell cycle arrest and mitochondrial apoptosis in Dalton's Lymphoma: An in vitro and in vivo study. J Ethnopharmacol. 2023; 302(Pt A):115849.
3. Sharma K, Kumar M, Waghmare R, et al. Moringa (Moringa oleifera Lam.) polysaccharides: extraction, characterization, bioactivities, and industrial application. Int J Biol Macromol 2022; 209(Pt A):763-78.
4. Aoki H, Nakatsuka-Mori T, Ueno Y, et al. Analysis of functional ingredients of tempe-like fermented Moringa oleifera seeds (Moringa tempe) prepared with Rhizopus species. J Biosci Bioeng 2023; 135(4):306-12.
5. Alavi M, Hamblin MR, Mozafari MR, et al. Surface modification of SiO2 nanoparticles for bacterial decontaminations of blood products. Cell Mol Biomed Rep 2022; 2(2):87-98.
6. Alavi M, Rai M, Martinez F, et al. The efficiency of metal, metal oxide, and metalloid nanoparticles against cancer cells and bacterial pathogens: different mechanisms of action. Cell Mol Biomed Rep 2022; 2(1):10-21.
7. Alavi M, Hamblin MR. Antibacterial silver nanoparticles: effects on bacterial nucleic acids. Cell Mol Biomed Rep 2023; 3(1):35-41.
8. Payal, Pandey P. Role of nanotechnology in electronics: a review of recent developments and patents. Recent patents on nanotechnology 2022; 16(1):45-66.
9. Li X, Shi C, Wang S, et al. Uncovering the effect of Moringa oleifera Lam. leaf addition to Fuzhuan Brick Tea on sensory properties, volatile profiles and anti-obesity activity. Food Funct 2023; 14(5):2404-15.
10. Nawaz QU, Kausar R, Jabeen N, et al. Influence of bio fabricated manganese oxide nanoparticles for effective callogenesis of Moringa oleifera Lam. Plant Physiol Biochem 2023; 198:107671.
11. Rasouli H, Popović-Djordjević J, Sayyed RZ, et al. Nanoparticles: A New Threat to Crop Plants and Soil Rhizobia? In: Hayat S, Pichtel J, Faizan M, et al., editors. Sustainable Agriculture Reviews 41: Nanotechnology for Plant Growth and Development. Cham: Springer International Publishing; 2020. p. 201-14.
12. Hasanuzzaman M, Raihan MRH, Nowroz F, et al. Insight into the physiological and biochemical mechanisms of biostimulating effect of Ascophyllum nodosum and Moringa oleifera extracts to minimize cadmium-induced oxidative stress in rice. Environmental science and pollution research international. 2023; 30(19):55298313.
13. Liu R, Liu J, Huang Q, et al. Moringa oleifera: a systematic review of its botany, traditional uses, phytochemistry, pharmacology and toxicity. The Journal of pharmacy and pharmacology 2022; 74(3):296-320.
14. Gali Aba Lulesa T, Beyene D, Ebba M, et al. Water treatment using natural coagulant and electrocoagulation process: A comparison study. Int J Anal Chem 2022; 2022:4640927.
15. Araujo NS, Souza NF, de Lima-Faria JM, et al. Treatment of cosmetic industry wastewater by flotation with Moringa oleifera Lam. and aluminum sulfate and toxicity assessment of the treated wastewater. Environmental science and pollution research international 2022; 29(1):1199-1209.
16. Marschner H. Zinc Uptake from soils. in: Robson AD, editor. zinc in soils and plants: proceedings of the international symposium on ‘zinc in soils and plants’ held at the University of Western Australia, 27–28 September, 1993. Dordrecht: Springer Netherlands; 1993. p. 59-77.
17. Abdoun K, Alsagan A, Altahir O, et al. Cultivation and uses of Moringa oleifera as non-conventional feed stuff in livestock production: a review. Life (Basel, Switzerland) 2022; 13(1):63.
18. Barba-Brioso C, Hidalgo PJ, Fernandez-Landero S, et al. Phytoaccumulation of trace elements (As, Cd, Co, Cu, Pb, Zn) by Nicotiana glauca and Euphorbia segetalis growing in a Technosol developed on legacy mine wastes (Domingo Rubio wetland, SW Spain). Environ Geochem Health. 2023.
19. Mawouma S, Ponka R, Mbofung CM. Acceptability and solubility of iron and zinc contents of modified Moringa oleifera sauces consumed in the Far-north region of Cameroon. Food Sci Nutr 2017; 5(2):344-8.
20. Yusefi-Tanha E, Fallah S, Rostamnejadi A, et al. Responses of soybean (Glycine max [L.] Merr.) to zinc oxide nanoparticles: Understanding changes in root system architecture, zinc tissue partitioning and soil characteristics. The Science of the total environment. 2022; 835:155348.
21. Lindsay W. Inorganic phase equilibria micronutrients in soils. Micronutrients in agriculture 1972:41-57.
22. Chen X, Zhang R, Xing Y, et al. The efficacy of different seed priming agents for promoting sorghum germination under salt stress. PLoS One. 2021; 16(1):e0245505.
23. Dehkourdi EH, Mosavi M. Effect of anatase nanoparticles (TiO2) on parsley seed germination (Petroselinum crispum) in vitro. Biological trace element research 2013; 155(2):283-6.
24. Wu F, Fang Q, Yan S, et al. Effects of zinc oxide nanoparticles on arsenic stress in rice (Oryza sativa L.): germination, early growth, and arsenic uptake. Environmental science and pollution research international 2020; 27(21):26974-81.
25. Baba SA, Malik SA. Determination of total phenolic and flavonoid content, antimicrobial and antioxidant activity of a root extract of Arisaema jacquemontii Blume. Journal of Taibah University for Science 2015; 9(4):449-54.
26. Hara K, Someya T, Sano K, et al. Antioxidant activities of traditional plants in Sri Lanka by DPPH free radical-scavenging assay. Data in brief 2018; 17:870-5.
27. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal biochem 1976; 72(1-2):248-254.
28. Chance B, Maehly A. [136] Assay of catalases and peroxidases. Methods in enzymology 1955; 2:764-75.
29. Mehlhorn H. Ethylene‐promoted ascorbate peroxidase activity protects plants against hydrogen peroxide, ozone and paraquat. Plant, Cell & Environment 1990; 13(9):971-6.
30. Jahantigh Haghighi z, fahmideh l, Fazeli Nasab b. Evaluation and comparison of leaf antioxidant properties and morphological traits of tomato varieties (Lycopersicon esculentum L). Journal of Iranian Plant Ecophysiological Research 2018; 13(50):63-76.
31. Anjum S, Vyas A, Sofi T. Fungi-mediated synthesis of nanoparticles: characterization process and agricultural applications. J Sci Food Agric 2023; 103(10):4727-41.
32. Patil S, Sastry M, Bharde A. Size and shape directed novel green synthesis of plasmonic nanoparticles using bacterial metabolites and their anticancer effects. Front Microbiol 2022; 13:866849.
33. Abdallah Y, Hussien M, Omar MOA, et al. Mung Bean (Vigna radiata) treated with magnesium nanoparticles and its impact on soilborne Fusarium solani and Fusarium oxysporum in clay soil. Plants (Basel) 2022; 11(11):1514.
34. Azeem M, Pirjan K, Qasim M, et al. Salinity stress improves antioxidant potential by modulating physio-biochemical responses in Moringa oleifera Lam. Sci Rep. 2023; 13(1):2895.
35. Rafiq H, Aftab ZE, Anjum T, et al. Bio-fabrication of zinc oxide nanoparticles to rescue mung bean against Cercospora leaf spot disease. Front Plant Sci 2022; 13:1052984.
36. Atteya AKG, El-Serafy RS, El-Zabalawy KM, et al. Exogenously supplemented proline and phenylalanine improve growth, productivity, and oil composition of salted moringa by up-regulating osmoprotectants and stimulating antioxidant machinery. Plants (Basel) 2022; 11(12):1553.
37. Jenish A, Ranjani S, Hemalatha S. Moringa oleifera nanoparticles demonstrate antifungal activity against plant pathogenic fungi. Appl Biochem Biotechnol 2022; 194(10):4959-70.
38. El-Khadragy M, Alolayan EM, Metwally DM, et al. Clinical efficacy associated with enhanced antioxidant enzyme activities of silver nanoparticles biosynthesized using Moringa oleifera leaf extract, against cutaneous leishmaniasis in a murine model of Leishmania major. Int J Environ Res Public Health 2018; 15(5):1037.
39. Shah G, Tu J, Fayyaz M, et al. Moringa oleifera smoke induced positive changes in biochemical, metabolic, and antioxidant profile of rice seedling under cadmium stress. Int J Phytoremediation 2022:1-11.
40. Wyszkowski M, Wyszkowska J, Kordala N, et al. Molecular sieve, halloysite, sepiolite and expanded clay as a tool in reducing the content of trace elements in Helianthus annuus L. on copper-contaminated soil. Materials (Basel, Switzerland) 2023; 16(5):1827.
41. Milla PG, Penalver R, Nieto G. Health Benefits of Uses and Applications of Moringa oleifera in Bakery Products. Plants (Basel). 2021; 10(2):318.
42. Watanabe S, Okoshi H, Yamabe S, et al. Moringa oleifera Lam. in diabetes mellitus: a systematic review and meta-analysis. Molecules. 2021; 26(12):3513.
43. Soumaoro I, Pitala W, Gnandi K, et al. Health Risk assessment of heavy metal accumulation in broiler chickens and heavy metal removal in drinking water using moringa Oleifera seeds in lome, Togo. Journal of health & pollution. 2021; 11(31):210911.
44. Abdelhameed RE, Abdel Latef AAH, Shehata RS. Physiological responses of salinized fenugreek (Trigonellafoenum-graecum L.) plants to foliar application of salicylic acid. Plants (Basel) 2021; 10(4):10.3390
45. Sehsah MD, El-Kot GA, El-Nogoumy BA, et al. Efficacy of Bacillus subtilis, Moringa oleifera seeds extract and potassium bicarbonate on Cercospora leaf spot on sugar beet. Saudi J Biol Sci. 2022; 29(4):2219-29.
| Files | ||
| Issue | Vol 11 No 5-6 (2023) | |
| Section | Original Articles | |
| DOI | https://doi.org/10.18502/jmb.v11i5-6.14359 | |
| Keywords | ||
| Moringa Olifera Capparis Spinosa Guaiacol Peroxidase Antioxidant Activity Ascorbate Peroxidase | ||
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How to Cite
1.
Khajeh H, Fazeli Nasab B, Mirzaei AR, Farzanfar H. Biosynthesis of Zinc Nanoparticles of Capparis Spinosa Plant Extract and the it’s Investigation on Morhpophysiological Properties of the Moringa Olifera Plant. J Med Bacteriol. 2023;11(5-6):17-29.
