Frequency of Shiga Toxin Associated Genes in Escherichia coli Isolated from Salivary Abomasum Disease in Kid Goats
Abstract
Background: Salivary abomasum disease (SAD) is a devastating disease causing significant mortality in Iranian goat and sheep herds. Understanding the causative agents is essential for developing effective preventive measures. This study investigated the potential role of Shiga toxin-producing Escherichia coli (STEC) in SAD pathogenesis.
Methods: We isolated E. coli from kid goats aged 3-30 days experiencing a sudden, acute illness characterized by gait imbalance, and death within 48 hours during the kidding season. Following isolation, we employed multiplex PCR to identify the presence of Shiga toxin genes (Stx1 and Stx2) associated with virulence in STEC strains.
Results: E. coli was isolated from 30 (75%) out of 40 animals. Notably, 7 (23.3%) isolates harbored the Stx2 gene, while only one isolate (3.3%) possessed the Stx1 gene.
Conclusion: These findings suggest a potential role for STEC, particularly strains carrying the Stx2 gene, in the development of SAD and multiple abomasal hemorrhages, in kid goats. The presence of Shiga toxin genes in a significant proportion of E. coli isolates highlights the importance of further research to elucidate their contribution to SAD pathogenesis and inform the development of targeted interventions.
2. Christodoulopoulos G. Salivary abomasum disease in young lambs. Vet Rec 2008; 162(22):732.
3. Christodoulopoulos G, Scott P, Jehl N, et al. Clinical, microbiological and histological findings in lambs affected by salivary abomasum disease. Vet Rec 2013; 172(4):100.
4. Han Y, Liu M, Han Y, et al. Genetic and phylogenetic characterization of Shiga toxin‐producing Escherichia coli and enteropathogenic E. coli from livestock in Jiangsu by using whole‐genome sequencing. J Appl Microbiol 2022; 132(5):3925-36.
5. Angell J, Duncan J. Watery mouth disease in neonatal lambs: a systematic literature review. Livestock 2020; 25(2):94-103.
6. Gascoigne E, Davies P. An approach to neonatal lamb post-mortem examinations. Livestock 2019; 24(4):193-8.
7. Kieckens E, Rybarczyk J, De Zutter L, et al. Clearance of Escherichia coli O157: H7 infection in calves by rectal administration of bovine lactoferrin. Appl Env Microbiol 2015; 81(5):1644-51.
8. Dwyer C, Conington J, Corbiere F, et al. Invited review: Improving neonatal survival in small ruminants: Science into practice. Animal 2016; 10(3):449-59.
9. Benavides J, González L, Dagleish M, et al. Diagnostic pathology in microbial diseases of sheep or goats. Vet microbiol 2015; 181(1-2):15-26.
10. Atef Yekta M. New strategies for prevention of E. coli O157: H7 infection in sheep: Ghent University; 2011.
11. Paton AW, Paton JC. Detection and characterization of Shiga toxigenic Escherichia coli by using multiplex PCR assays for stx 1, stx 2, eaeA, enterohemorrhagic E. coli hlyA, rfb O111, and rfb O157. J Clin Microbiol 1998; 36(2):598-602.
12. Eckel EF, Ametaj BN. Bacterial endotoxins and their role in periparturient diseases of dairy cows: mucosal vaccine perspectives. Dairy 2020; 1(1):61-90.
13. Ghanbarpour R, Askari N, Ghorbanpour M, et al. Genotypic analysis of virulence genes and antimicrobial profile of diarrheagenic Escherichia coli isolated from diseased lambs in Iran. Trop Anim Health Prod 2017; 49:591-7.
14. Ghanbarpour R, Kiani M. Characterization of non-O157 shiga toxin-producing Escherichia coli isolates from healthy fat-tailed sheep in southeastern of Iran. Trop Anim Health Prod 2013; 45:641-8.
15. van Hoek AH, Lee S, van den Berg RR, et al. Virulence and antimicrobial resistance of Shiga toxin-producing Escherichia coli from dairy goat and sheep farms in The Netherlands. J Appl Microbiol 2023; 134(6):lxad119.
16. Naderi Z, Ghanbarpour R, Jajarmi M, et al. Antibiotic resistance profiling and phylotyping of human-diarrheagenic Escherichia coli pathotypes detected from diarrheic and non-diarrheic calves in Iran. Mol Biol Rep 2024; 51(1):1-11.
17. Arrais BR, Silveira ÂV, Oliveira AF, et al. Stx1 and Stx2 subtyping and antimicrobial resistance in Shiga toxin-producing Escherichia coli (STEC) isolates from cattle and sheep feces in the Southeastern region of the State of Goiás, Brazil. Pesqui Vet Bras 2021; 41:e06747.
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Issue | Vol 12 No 4 (2024) | |
Section | Original Articles | |
DOI | https://doi.org/10.18502/jmb.v12i4.17002 | |
Keywords | ||
Escherichia coli Goat Salivary abomasum disease Shiga toxin. |
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