Original Articles

Prevalence and Molecular Typing of Non-Tuberculous Mycobacteria in Hospital Water Sources of Tehran, Iran

Abstract

Background:   Non-tuberculous mycobacteria (NTM) pose a growing public health concern, especially in regions with high prevalence rates. Iran, situated near high-burden countries like Afghanistan and Pakistan, is particularly vulnerable. This study aimed to investigate the molecular epidemiology of NTM in water samples from Farhikhtegan Hospital, Tehran.

Methods:   A total of 70 water samples were collected from various hospital departments. After culture and phenotypic identification, 33 mycobacterial isolates were subjected to polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of the rpoB gene.

Results:   Mycobacterium fortuitum type I was the most prevalent NTM species, accounting for 81% of the isolates. Mycobacterium kansasii type I and Mycobacterium gordonae type I followed, each comprising 6% of the isolates. Less common species included M. gordonae type II and Mycobacterium intracellulare. The PCR-RFLP method proved to be a sensitive and accurate tool for identifying NTM species.

Conclusion:   This study provides valuable insights into the NTM profile in Farhikhtegan Hospital and underscores the importance of effective surveillance and control measures to mitigate the risk of NTM infections.

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IssueVol 13 No 2 (2025) QRcode
SectionOriginal Articles
Keywords
Non-tuberculosis mycobacterium PCR-RFLP rpoB gene Water.

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How to Cite
1.
Mahmoudi P, Amini K, Falsafl S. Prevalence and Molecular Typing of Non-Tuberculous Mycobacteria in Hospital Water Sources of Tehran, Iran. J Med Bacteriol. 2025;13(2):31-39.