Original Articles

Characterization of E. coli Isolate and Assessment of its Antibiotic Susceptibility against Colistimethate Sodium

Abstract

Background:   The increasing application of Escherichia coli as a model organism in microbiological quality control highlights importance of E. coli due to their wide range of applications. However, rising antimicrobial resistance in E. coli produced a serious concern with respect to public health globally which eventually generated the need of screening of pathogens for their sensitivity and resistance profile against different antibiotics.

Methods:   The present study isolated a bacterial strain of E. coli, SA-01 from Ghaziabad, Uttar Pradhesh, India which was then characterized based on morphology, microscopy, biochemical testing in comparison with standard reference strains of E. coli (ATCC 8739, ATCC 10536 and MTCC 448). The identity of the isolated strain was further confirmed by MALDI-TOF analysis. The antibiotic susceptibility of the isolated strain of E. coli (SA-01) was then assessed in comparison with E. coli ATCC 10536 by cup plate method using different antibiotic doses of colistimethate sodium (1, 5, 10, 20, 30, 40, 50, 100 and 461 µg/mL).

Results:    Based on phenotypic and biochemical characteristics, isolated bacterial strain, SA-01 was identified as E. coli and was further authenticated by MALDI-TOF analysis. In the antibiotic susceptibility testing, the indigenous strain of E. coli (SA-01) has shown zone of inhibition similar to E. coli ATCC 10536.

Conclusion: Similar zone of inhibition compared to E. coli ATCC 10536 in the antibiotic susceptibility testing, revealed sensitivity profile of the indigenous strain of E. coli (SA-01) to Colistimethate sodium suggesting its potential use in microbiological quality control methods.

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IssueVol 13 No 4 (2025) QRcode
SectionOriginal Articles
Keywords
Antimicrobial susceptibility Characterization Colistimethate sodium E. coli.
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How to Cite
1.
Parashar K, Teotia AK, Thota P, Lagashetti A, Pandey M, Keshari A, Dahiya M, Kalaiselvan V. Characterization of E. coli Isolate and Assessment of its Antibiotic Susceptibility against Colistimethate Sodium. J Med Bacteriol. 2025;13(4):56-66.