Determination of Inhibitory Concentration of Oxytetracycline on Methanogenic Bacteria by In Vitro Study
Abstract
Introduction: Antibiotics have the potential to adversely affect the microbial community. Foranaerobic digestion, a sufficient methanogenic population needs to be preserved in the system.
The main aim of this study was determination of inhibitory concentration of oxytetracycline on
methanogenic bacteria.
Methods: A 120 mL jacketed bioreactor with a 90 mL working volume was inoculated granular
sludge from an anaerobic digester, substrate and different concentration of oxytetracycline with
10 days cycles and intermittent mixing. The reactor was operated at 35 ± 2 ° C. The inhibitory
effect of antibiotic was evaluated by monitoring biogas production.
Results: Based on the findings from each batch, complete inhibitory concentration of oxy
tetracycline was in concentration of 800 mg L-1. Significant relation was seen between
inoculated antibiotic concentrations and methane production (r=-0.86).
Conclusion: The addition of antibiotics to the biomass affected the utilization of fatty acids,
resulting in unfavorable effects on methanogenesis. Thus, overusing of antibiotics can adverse
effects of intestinal flora.
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| Files | ||
| Issue | Vol 4 No 1-2 (2015) | |
| Section | Original Articles | |
| Keywords | ||
| Antibiotic Oxytetracycline Methanogen bacteria | ||
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