Phenotypic and Molecular Detection of Pathogenic Vibrio Species in Two Different Regions of the Caspian Sea in Mazandaran, Iran
-
Bahareh Lashtoo Aghaee
,
Mohammad Hassan Shirazi
,
Mohammad Reza Pourmand
,
Mostafa Hosseini
,
Davood Afshar
,
Sara Hajikhani
,
Saeedeh Shobeiri
Abstract
Background: Vibrio species including V. cholera, V. mimicus, V. parahaemolyticus, and V. vulnificus may cause gastrointestinal diseases after seafood consumption, and wound infections in swimmers and fishermen after exposing to seawater. This study determined the prevalence of the four Vibrio species in Tonkabon and Ramsar recreational beach water (approximately 200 meter far from the place where the river reaches the sea) and estuaria (place where the river reaches the sea) in northern part of Iran from autumn 1391 through autumn 1392.
Methods: Three hundreds water samples were collected for the detection of Vibrio species, using biochemical identification.
Results: Genomic DNA extracted from isolates and 16Sr DNA PCR confirmed the successful isolation of 9 Vibrio species in recreational beach water region.
Conclusion: Out of 300 samples, nine positive samples include one V. cholerae and eight V. parahaemolyticus were found at recreational beach (approximately 200 meter far from the place where the river reaches the sea).
Halakou A, Amir Mozafari N, Forouhesh Tehrani H. Distribution of vibrio species in Caspian seawater. J Ofogh Danesh 2005; 11(3): 16-20.
Dickinson G, Lim KY, Jiang SC. Quantitative microbial risk assessment of pathogenic vibrios in marine recreational waters of southern California. Appl Environ Microbiol 2013; 79(1): 294-302.
Diaz JH. Skin and Soft Tissue Infections Following Marine Injuries and Exposures in Travelers. J Travel Med 2014; 21(3): 207-13.
Schets F. M, Van Den Berg HH, Marchese A, et al. Potentially human pathogenic vibrios in marine and fresh bathing waters related to environmental conditions and disease outcome. Int J Hyg Environ Health 214; 2011: 214(5): 399-406.
Asakura H, Ishiwa A, Arakawa E, et al. Gene expression profile of Vibrio cholerae in the cold stress-induced viable but non-culturablestate. Environ Microbiol 2007; 9(4): 869-79.
Collin, B. & Rehnstam-Holm, A. S. Occurrence and potential pathogenesis of Vibrio cholerae, Vibrio parahaemolyticus and Vibrio vulnificus on the South Coast of Sweden. FEMS Microbiol 2011; 78(2): 306-313.
Torp-Pedersen, T., Nielsen, X. C., Olsen, K. E. & Barfod, T. S. Intracerebral abscess after infection with non-toxigenic Vibrio cholera. Ugeskr Laeger 2012; 174 (8): 498-9.
Barton JC and Acton RT. Hemochromatosis and Vibrio vulnificus wound infections. J Clin Gastroenterol 2009; 43(9): 890-3.
Canigral I, Moreno Y, Alonso JL, et al. Detection of Vibrio vulnificus in seafood, seawater and wastewater samples from a Mediterranean coastal area. Microbiol Res 2009; 43(9): 890-3.
Baizabal-Ramirez, O. Negrete-Pérez M, Guerrero-Daza D, et al. Septic shock by Vibrio vulnificus at the coast Gulf of Mexico. Rev Med Inst Mex Seguro Soc 2011; 49 (4): 433-6.
Ottaviani D, Leoni F, Serra R, et al. Nontoxigenic Vibrio parahaemolyticus strains causing acute gastroenteritis. J Clin Microbiol 2012; 50(12): 4141-3.
Urmersbach S, Alter T, Sanjeevani Gonsal Koralage M, et al. Population analysis of Vibrio parahaemolyticus originating from different geographical regions demonstrates a high genetic diversity. BMC Microbiol 2014; 14(1): 59.
Tena, D. Arias M, Alvarez BT, et al. Fulminant necrotizing fasciitis due to Vibrio parahaemolyticus. J Med Microbiol 2010; 59 (2): 235-8.
Eyisi OA, Uchechukwu UN, Christian UI. Distribution of vibrio species in shellfish and water samples collected from the Atlantic coastline of south-east Nigeria. J Health Popul Nutr 2013; 31(3): 314-20.
Böer SI, Heinemeyer EA, Luden K, et al. Temporal and Spatial Distribution Patterns of Potentially Pathogenic Vibrio spp. at Recreational Beaches of the German North Sea. Microb Ecol 2013: 65(4): 1052-67.
Maria E. Asplund. Ecological aspects of marine Vibrio bacteria exploring relationships to other organisms and a changing environment 2013. ISBN: 91-89677-52-8.
Vezzulli L, Pezzati E, Moreno M, et al. Benthic ecology of Vibrio spp. and pathogenic Vibrio species in a coastal Mediterranean environment (La Spezia Gulf, Italy). Microbiol Ecol 2009; 58(4): 808-18.
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| Issue | Vol 4 No 3-4 (2015) | |
| Section | Original Articles | |
| Keywords | ||
| Caspian Sea Pathogenic Vibrio Species 16S rDNA PCR | ||
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