Original Articles

Identification and Extraction of Chicken Egg Yolk Immunoglobulin from Egg by Polyethylene Glycol (PEG) Precipitation

Abstract

Background: Staphylococcus aureus strains exhibiting multiple antibiotic resistances are isolated from most communities and hospital infections. Treatment of patients with these infections has been difficult. The aim of this study was to detect and extract, the egg yolk immunoglobulin Y as a potential source of anti- S. aureus antibody.

 Methods: Specific IgY was produced by immunizing hens with formalin-killed S. aureus. The specificity of serum`s antibody was confirmed by ELISA method. The antibodies were extracted from egg yolk by polyethylene glycol (PEG) precipitation. Proteins were analysed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).

 Results: Chicken egg yolk antibodies (IgY) were raised against S. aureus in the serum after injections. Up to 104 dilution specific antibodies were determined in serum.

 Conclusion: The results of the ELISA indicates the specificity of the immunoglobulin Y to the target antigen. In order to find a viable alternative to antibiotic treatments, more research must be done on the ability of these antibodies to inhibit the growth of S. aureus.

Honeyman A, Friedman H, Bendinelli M. Staphylococcus aureus infection and disease. Springer. 2002.

Stranger-Jones YK, Bae T, Schneewind O. Vaccine assembly from surface proteins of Staphylococcus aureus. Proc Natl Acad Sci

USA. 2006; 103: 16942-16947.

Guimarães M, Amaral LG, Rangel LBA, et al. Growth inhibition of Staphylococcus aureus by chicken egg yolk antibodies. Arch. Immunol Ther. Exp 2009; 57: 377–382.

Michael A, Meenatchisundaram S, Parameswari G, et al. Chicken egg yolk antibodies (IgY) as an alternative to mammalian antibodies. Indian J.Sci.Technol 2010; 3(4): 468-474.

Meenatchisundaram S, Michael A, Subbraj T, et al. Isolation, Purification and Neutralizing potential of chicken egg yolk immunoglobulin (IgY) against mastitis causing Escherichia coli in dairy cows in Coimbatore District. Int. j. drug dev. res 2011; 3: 147-153.

Liou JF, Shiau JW, Tai C, et al. Production of Egg Yolk Immunoglobulin against Escherichia coli from White Leghorn and Lohmann Chickens. J Anim Vet Adv 2011; 10(18): 2349-2356.

Herbert WJ. Methods for the preparation of waterin-oil and multiple emulsions for use as antigen adjuvants, and notes on their use in immunization procedures. In Handbook of Exprimental Immunology (ed. D.M. Weir). 1967; 1207-1214.

Sunwoo HH, Li X, Lee EN, et al. Egg Nutrition and Biotechnology. 2000; 311–322.

Polson A, Von Wechmar MB, Van Regenmortel MH. Isolation of viral IgY antibodies from yolks of immunized hens. Immunol Commun 1980; 9: 475-493.

Laemmeli, UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227: 680 - 685.

Pauly D, Dorner M, Zhang X, et al. Monitoring of laying capacity, immunoglobulin Y concentration, and antibody titer development in chickens immunized with ricin and botulinum toxins over a two-year period. Poult Sci 2009; 88: 281-290.

Meulenaer DB, Baert K, Lanckriet H, et al. Development of an enzyme-linked immunosorbent assay for bisphenol A using chicken immunoglobulins. J. Agric. Food Chem 2002; 50: 5273-5282.

Hirai K, Arimitsu H, Umeda K, et al. Passive oral immunization by egg yolk immunoglobulin (IgY) to Vibrio cholerae effectively prevents cholera. Acta Med Okayama 2010; 64: 163-170.

Grey HM. Duck immunoglobulins. II. Biologic and immunochemical studies. Eur. J. Immunol 1967; 98: 820-826.

Clifton-Hadley FA, Breslin M, Venables LM, et al. A laboratory study of an inactivated bivalent iron restricted Salmonella enterica serovars Enteritidis and Typhimurium dual vaccine against Typhimurium challenge in chickens. Vet Microbiol 2002; 89: 167-79.

Pauly D, Chacana PA, Calzado EG, et al. IgY Technology: Extraction of Chicken Antibodies from Egg Yolk by Polyethylene Glycol (PEG) Precipitation. Journal of Visualized Experiments 2011; 1-5.

Polson A. Isolation of IgY from the yolks of eggs by a chloroform polyethylene glycol procedure. Immunol Invest 1990; 19(3): 253-258.

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IssueVol 4 No 5-6 (2015) QRcode
SectionOriginal Articles
Keywords
Staphylococcus aureus Serum ELISA Polyethylene glycol.
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How to Cite
1.
Soltan Dallal MM, Kalantar E, Kafami Khorasani L, Hoshdar Tehrani MH, Zeinolabedini Zamani M. Identification and Extraction of Chicken Egg Yolk Immunoglobulin from Egg by Polyethylene Glycol (PEG) Precipitation. J Med Bacteriol. 2015;4(5-6):13-18.