Determination of the Status of Helicobacter pylori sabA Gene in Relation to Clinical Findings
,
Abstract
Background: Many Helicobacter pylori strains express adhesin proteins that bind to specific host-cell macromolecule receptors, like sialic acid binding adhesion (sabA). SabA-expressing strains have been associated with gastric cancer and negatively associated with duodenal ulcers. The aim of this study was to determine the status of sabA gene of H. pylori and its association with the clinical diseases in Iranian dyspeptic pateints.
Methods: Eighty six biopsy block samples that were positive for H. pylori according Geimsa staining were included in this study. Genomic DNA was extracted from paraffin-embedded gastric biopsies obtained from dyspeptic patients. The identity of Helicobacter genus was determined through amplification of 16S rRNA which followed by sabA PCR using the gene-specific primers. The prevalence of sabA gene in three clinical groups including gastritis, gastric ulcer, and gastric atrophy was determined. The association of sabA gene and clinical outcomes was assessed statistically using Chi-square test. A p-value less than <0.05 was considered statistically significant.
Results: Total of 86 patients was included in this study. Seventeen cases out of 86 (23.6%) were yielded a positive result for sabA gene. The prevalence of sabA gene was 28.6% in both dyspeptic and Gastric atrophy patients as compared with peptic ulcers (19.2%).
Conclusion: For a first time the frequency of sabA gene using PCR methods was reported. The current study demonstrated that the sabA gene status was not associated with clinical diseases. In limited number of studied samples, higher frequency of sabA gene among dyspeptic and atrophic patients was found.
McColl KEL, El-Omar E, Gillen D. Helicobacter pylori gastritis and gastric physiology. Gastroenterol Clin North Am 2000; 29 (3): 687-703.
Peterson WL, Feldman M. Helicobacter pylori and peptic ulcer disease. N Engl J Med 1991; 324 (15): 1043-8.
Uemura N, Okamoto S, Yamamoto S, et al.
Helicobacter pylori infection and the development of gastric cancer. N Engl J Med 2001; 345 (11): 784-9.
Blaser MJ, Perez-Perez GI, Kleanthous H, et al.Infection with Helicobacter pylori strains possessing cagA is associated with an increased risk of developing adenocarcinoma of the stomach. Cancer Res 1995; 55 (10): 2111-5.
Kuipers EJ, Pérez-Pérez GI, Meuwissen SGM, et al. Helicobacter pylori and atrophic gastritis: importance of the cagA status. J Natl Cancer Inst 1995; 87 (23): 1777-80.
Nomura AMY, Lee J, Stemmermann GN, et al.Helicobacter pylori CagA seropositivity and gastric carcinoma risk in a Japanese American population. J Infect Dis 2002; 186 (8): 1138-44.
Parsonnet J, Friedman GD, Orentreich N, et al.Risk for gastric cancer in people with CagA positive or CagA negative Helicobacter pylori infection. Gut 1997; 40 (3): 297-301.
Rugge M, Busatto G, Cassaro M, et al. Patients younger than 40 years with gastric carcinoma. Cancer 1999; 85 (12): 2506-11.
Van Doorn LJ, Figueiredo C, Megraud F, et al.Geographic distribution of vacA allelic types of Helicobacter pylori. Gastroenterology 1999; 116 (4): 823-30.
Yamaoka Y, Kodama T, Gutierrez O, et al.Relationship between Helicobacter pylori iceA, cagA, and vacA status and clinical outcome: studies in four different countries. J Clin Microbiol 1999; 37 (7): 2274-9.
Molaei M, Foroughi F, Mashayekhi R, et al.CagA status and VacA subtype of Helicobacter pylori in relation to histopathologic findings in Iranian population. Indian J Pathol Microbiol 2010; 53 (1): 24-7.
Douraghi M, Saberi Kashani S, Shokrgozar M.A, et al. Characterization of the Vacuolating Cytotoxin in Helicobacter pylori Strains Isolated from Iran. Yakhteh Med J 2010; 12 (1): 1-6.
Chattopadhyay S, Datta S, Chowdhury A, et al.Virulence genes in Helicobacter pylori strains from West Bengal residents with overt H. pylori- associated disease and healthy volunteers. J Clin Microbiol 2002; 40 (7): 2622-5.
Mahdavi J, Sondén B, Hurtig M, et al.Helicobacter pylori SabA adhesin in persistent infection and chronic inflammation. Science 2002; 297 (5581): 573-8.
Aspholm M, Olfat FO, Nordén J, et al. SabA is the H. pylori hemagglutinin and is polymorhic in binding to sialylated glycans. PLoS Pathog 2006;2 (10): e110.
Unemo M, Spholm-Hurtig M, Ilver D, et al. The sialic acid binding SabA adhesin of Helicobacter pylori is essential for nonopsonic activation of human neutrophils. J Biol Chem 2005; 280 (15):15390-7.
Xuan SY, Li N, Qiang X, et al. Helicobacter infection in hepatocellular carcinoma tissue. World J Gastroenterol 2006; 12 (15): 2335-40.18.
Lehours P, Ménard A, Dupouy S, et al.Evaluation of the association of nine Helicobacter pylori virulence factors with strains involved in low-grade gastric mucosa-associated lymphoid tissue lymphoma. Infect Immun 2004;72 (2): 880-8.
Yamaoka Y. Increasing evidence of the role of Helicobacter pylori SabA in the pathogenesis of gastroduodenal disease. J Infect Dev Ctries 2008;2 (3): 174-81.
Mikaeli J, Malekzadeh R, Ziad-Alizadeh B, et al.Prevalence of Helicobacter pylori in two Iranian provinces with high and low incidence of gastric carcinoma. Arch Iran med 2000; 3 (1): 6-9.
Shirazi M H Study of Helicobacter pylori prevalence in patients with gastrointestinal lesions referred to Tehran hospitals. Transplant Proc 2003; 39 (4): 1003-7.
Falsafi T, Favaedi R, Mahjoub F, et al.Application of stool-PCR test for diagnosis of Helicobacter pylori infection in children. World J Gastroenterol 2009; 15 (4): 484-8.
Yanai A, Maeda S, Hikiba Y, et al. Clinical relevance of Helicobacter pylori sabA genotype in Japanese clinical isolates. J Gastroenterol Hepatol 2007; 22 (12): 2228-32.
Misra V, Misra S, Dwivedi M, et al. A topographic study of Helicobacter pylori density, distribution and associated gastritis. J Gastroenterol Hepatol 2000; 15 (7): 737-43.
Genta RM, Graham DY. Comparison of biopsy sites for the histopathologic diagnosis of Helicobacter pylori: a topographic study of H. pylori density and distribution. Gastrointest Endosc 1994; 40 (3): 342-5.
| Files | ||
| Issue | Vol 1 No 1-2 (2012) | |
| Section | Original Articles | |
| Keywords | ||
| Helicobacter pylori SabA protein Peptic ulcer Dyspepsia Stomach Diseases RNA Ribosomal 16S | ||
| Rights and permissions | |
|
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
