Characterization of Anti-E.Coli Antibody Exteracted From Immunized Hen Eggs By Polyethylene Glycol (PEG) Precipitation

  • Mohammad Mehdi Soltan Dallal Division of Food Microbiology, Department of pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
  • Monireh Zeinolabedini Zamani Food Microbiology Research Center, Tehran University of Medical Sciences, Tehran, Iran.
  • Laya Kafami Khorasani Dietary Supplements and Probiotic Research Center, Alborz University of Medical Sciences, Karaj, Iran.
  • Mohammad Hassan Houshdar Tehrani Medicinal Chemistry Department, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
  • Mohammad Kazem Sharifi Yazdi Zoonosis Research Center, Department of Medical Laboratory Sciences, Tehran University of Medical Sciences, Tehran, Iran.
  • Saeid Vahedi Instructor of Department of anesthesia, School of Allied Medicine, Tehran University of Medical Sciences, Tehran, Iran.
  • Farnaz Soltani Jonaghani Food Microbiology Research Center, Tehran University of Medical Sciences, Tehran, Iran.
  • Fatemehsadat Mirsane Food Microbiology Research Center, Tehran University of Medical Sciences, Tehran, Iran.
Keywords:
Enzyme-Linked Immunosorbent Assay, Escherichia coli, Polyethylene Glycol, Serum

Abstract

Background: The aim of this study was to provide a simple and efficient way of producing IgY antibodies against Escherichia coli and extract that by polyethylene glycol (PEG) precipitation method.Methods: An ti- Escherichia coli antibody was produced in hens by using formalin-killed E.coli and confirmed by ELISA method, applying serum`antibody. The specific IgY was extracted from egg yolk by Polyethylene Glycol (PEG) Precipitation and analysed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).Results: The result of ELISA shows that the IgY titre increased from initial immunization and the high titre (≥0.071) persisted after the first immunization. Heavy chain of IgY with ~68 KDa was observed in the Gel electrophoresis pattern.Conclusion: The results of the ELISA indicates the specificity of the immunoglobulin Y to the target antigen and the result of SDS-PAGE represented the appropriate extraction method. More research must be done on the ability of these antibodies to inhibit the growth of E.coli.

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Published
2019-09-08
How to Cite
1.
Soltan Dallal MM, Zeinolabedini Zamani M, Kafami Khorasani L, Houshdar Tehrani MH, Sharifi Yazdi MK, Vahedi S, Soltani Jonaghani F, Mirsane F. Characterization of Anti-E.Coli Antibody Exteracted From Immunized Hen Eggs By Polyethylene Glycol (PEG) Precipitation. J Med Bacteriol. 8(5-6):38-43.
Section
Original Articles