Original Articles

Frequency of IMP-1 and VIM Genes among Metallo-beta-Lactamase Producing Acinetobacter spp. Isolated from Health Care Associated Infections inNortheast of Iran


Background: The emergence and rapid spread of metallo-beta-lactamase (MBL) producing Acinetobacter spp. are of great concern worldwide due to limited treatment options. Epidemiologic studies of the causing genes are important for prevention.Methods:  In  this  study,  70  imipenem-resistant  Acinetobacter  strains  were isolated from health care associated infections. These isolates were screened for detection of metallo-beta-lactamase (MBL) using inhibitor potentiated disk diffusion tests with thylenediaminetetraacetic acid (EDTA). PCR was designed for detection of bla vim and bla imp-1 using specific primers.Results: Among these 70 strains, 50 strains appeared to produce metallo-beta-lactamase. Three isolates were detected by PCR to carry metallo-beta-lactamase gene bla vim, but bla imp-1 gene was not detected.Conclusion: These findings suggest that in our area other genetic elements are responsible for resistance against metallo-beta-lactams.

Kuoa HY, Changc KC, Kuoe JW, et al. Imipenem: a potent inducer of multidrug resistance in Acinetobacter baumannii. Int J AntimicrobAgents 2011; 32: 854-9.

Lee k, Yong D, Chong Y. Multidrug- Resistant Acinetobacter spp. Increasingly Problematic Nosocomial Pathogens. Yonsei Med 2011; 52: 879-91.

Liu SY, Lin JY, Chu C, et al. Integron-associated imipenem resistance in Acinetobacter baumannii isolated from a regional hospital in Taiwan. Int J AntimicrobAgents 2006; 27: 81-4.

Mezzatesta ML, Migliavacca R, Giani T, et al. Epidemiological characterization and distribution of carbapenem resistant Acinetobacter baumannii clinical isolates in Italy.Bacteriology 2011; 18: 160-6.

Shahcheraghi F, Abbasalipour M, Feizabadi MM, et al. Isolation and genetic characterization of metallo - β -lactamase and carbapenamase producing strains of Acinetobacter baumannii from patients at Tehran hospitals. Iran J Microbiol 2011; 2 (3): 68-74.

Hujer M, Hujer A, Hulten E, et al.Analysis of Antibiotic Resistance Genes in Multidrug-Resistant Acinetobacter spp. Isolates from Military and Civilian Patients Treated at the Walter Reed Army Medical Center. Antimicrob Agents Chemother 2006; 50: 4114-23.

Lee YT, Huang L, Chiang D, et al.Differences in phenotypic and genotypic characteristics among imipenem non susceptible Acinetobacter isolates belonging to different genomic species in Taiwan. Int J Antimicrob Agents 2009; 34:580-4.

Brown NG, Horton LB, Huang W, et al. Analysis of the Functional Contributions of Asn 233 in Metallo-beta-Lactamase IMP-1. Antimicrob Agents Chemother 2011; 55: 5696-702.

Lee K, Yong D, Yum JH, et al.Evaluation of E-test MBL for Detection of blaIMP-1 and blaVIM-2 Allele-Positive Clinical Isolates of Pseudomonas spp. and Acinetobacter spp. J Clin Microbiol 2005; 43: 2-944.

Ikonomidis A, Ntokou E, Maniatis AN, et al. Hidden VIM-1 Metallo- beta-Lactamase Phenotypes among Acinetobacter baumannii Clinical Isolates. J Clin Microbiol 2008;46: 346-9.

Marie Kempf, Jean-Marc Rolain, Emergence of resistance to carbapenems in Acinetobacter baumannii in Europe: clinical impact and therapeutic options. Inter J Antimicrob Agents 2012; 39: 105-14.

Clinical and Laboratory Standards Institute M100-S17. Performance standards for antimicrobial susceptibility testing. Seventeenth infor-mational supplement. Wayne, PA: CLSI, 2007.

Lee K, Lee HS, Jang SJ, et al.Antimicrobial resistance surveillance of bacteria in 1999 in Korea with a special reference to resistance of enterococci to vancomycin and gram- negative bacilli to third generation cephalosporin, imipen-em, and fluoroquinolone. J Korean Med Sci 2001; 16: 262-70.

Lee K, Ha GY, Shi BM, et al.Metallo Beta lactamase-producing Gram-negative bacilli in Korean Nationwide Surveillance of Antimicrobial Resistance group hospitals in 2003: continued prevalence of VIM-producing Pseudomonas spp. and increase of IMP-producing Acinetobacter spp. Diagn Microbiol Infect 2004; 50: 51-8.

Johann D, Pitout D, Daniel B, et al.Detection of Pseudomonas aeruginosa Producing Metallo-beta-Lactamases in a Large Centralized Laboratory. J Clin Microbiol 2005; 43: 3129-35.

Amudhan MS, Sekar U, Kamalanathan A, et al. blaIMP and blaVIM mediated carbapenem resistance in Pseudomonas and Acinetobacter species in India. J Infect Dev Ctries 2012; 6: 757-62.

Yamamoto M, Nagao M, Matsumura Y, et al. Regional dissemination of Acinetobacter species harbouring metallo-b-lactamase genes in Japan. Clin Microbiol Infect 2012; 42: 1-8.

Lee MF, Peng CF, Hsu HJ, et al.Molecular characterisation of the metallo-beta-lactamase genes in imipenem-resistant Gram-negative bacteria from a university hospital in southern Taiwan. Int J Antimicrob Agents 2008; 32: 475-80.

Lee J, Lee W. VIM- and IMP-Type Metallo-B-lactamase Producing Pseudomonas spp. and Acinetobacter spp. in Korean Hospitals. Emerging Infect Dis 2003; 9: 868-71.

Shahcheraghi F, Abadean M.Molecular detection of genes bla VIM, bla SPM, bla IMP resistant to imipenem in Acinetobacter strains isolated from clinical specimens. J Trop Dis 2012; 57: 29-33.

Peymani A, Nahaei MR, Faragnia S, et al. High Prevalence of Metallo-Beta-Lactamase Producing Acinetobacter baumannii in a Teaching Hospital in Tabriz, Iran. Iran J Microb 2011; 64: 69-71.

IssueVol 2 No 3-4 (2013) QRcode
SectionOriginal Articles
Acinetobacter Carbapenemase Iran

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How to Cite
Noori N, Vandyosefi J, Sabet F, Ashrafi S, Ghazvini K. Frequency of IMP-1 and VIM Genes among Metallo-beta-Lactamase Producing Acinetobacter spp. Isolated from Health Care Associated Infections inNortheast of Iran. J Med Bacteriol. 2015;2(3-4):11-16.